Identification of Keratinophilic Fungi in Urban Waste and Cattle Field Soil of Kanpur, India for Environmental Pollution Management

Kanpur is a city which has huge number of leather product units and leather processing plants. These units are one of major contributors of keratinous waste and produces keratinous material as waste in the form of hairs, hides, dermis. During the present study 83 keratinophilic fungi were isolated from 40 soil samples of urban waste and cattle field habitat of various localities. From 20 samples of urban waste, 44 keratinophilic fungi were isolated, 39 fungi recorded from Cattle field. The frequency of genera Chrysosporium was recorded in urban waste (29.54%) and cattle field soil (20.51%). Maximum (13.83%) frequency was recorded in the case of Chrysosporium indicum in urban waste.

Keratinases Produced by Aspergillus stelliformis, Aspergillus sydowii, and Fusarium brachygibbosum Isolated from Human Hair: Yield and Activity

Twenty fungal strains belonging to 17 species and isolated from male scalp hair were tested for their capacity to hydrolyze keratinous material from chicken feather. The identification of the three most efficient species was confirmed by sequencing of the internal transcribed spacer (ITS) region of rDNA. Activities of fungal keratinases produced by Aspergillus stelliformis (strain AUMC 10920), A. sydowii (AUMC 10935), and Fusarium brachygibbosum (AUMC 10937) were 113, 120, and 130 IU mg−1 enzymes, respectively. The most favorable conditions were at pH 8.0 and 50 °C. Keratinase activity was markedly inhibited by EDTA and metal ions Ca+2, Co+2, Ni+2, Cu+2, Fe+2, Mg+2, and Zn+2, with differences between the fungal species. To the best of our knowledge, this is the first study on the activity of keratinase produced by A. stelliformis, A. sydowii, and F. brachygibbosum. F. brachygibbosum keratinase was the most active, but the species is not recommended because of its known phytopathogenicty. Aspergillus sydowii has many known biotechnological solutions and here we add another application of the species, as producer of keratinases. We introduce A. stelliformis as new producer of active fungal keratinases for biotechnological solutions, such as in the management of keratinous waste in poultry industry.

Keratinolysis of chicken feather and human hair by nondermatophytic keratinophilic fungi isolated from soil

Development in food industry increases consumption of chicken by people and it is estimated that tons of poultry feathers are produced by poultry farms. Hairs are other forms of keratinous waste which is generated in huge amounts by leather industries and parlours worldwide. Chicken feathers and hairs are waste contains high-quality protein, hard to degraded. Eleven nondermatophytic keratinophilic fungi were isolated from soil by hair baiting method and were used to deteriorate hairs and feathers. Pictographic authentication showed that the microbial incidence started with surface colonization of keratinous substrate, mechanical interference of substrate by penetrating hyphae and development of broad perforating organs. Fourier Transform Infrared Spectroscopy (FTIR) analysis of degraded and undegraded hair and the feather was made. In the sulphoxide region at 1073, the band corresponding to S-O was observed with low intensity and poorly visible in control feathers, while in degraded feather intensity of the band was high in case of Chrysosporium indicum and Chrysosporium tropicum. In Hairs, S-O band was more intense in C indicum as compared to C. tropicum while it was absent in undegraded human hair. The present work observed keratin degradation activity on human hair and chicken feather by FTIR spectra which are useful in the study of structure and mechanism of keratinolysis. Keratinous waste degradation has great potential to convert them into various byproducts such as enzymes, amino acids, biofertilizer and animal feed.

Bio-degradation of Feathers Waste by the Staphylococcus epidermidis P3A

Development of industrialization is directly proportional to the development of nations. Rising industrializations also increase the pollutions. One of them is poultry industry which discharges a huge amount of keratinous waste. The present study focused on the selection of feather degrading bacteria from Barshi region of Maharashtra. Bacterial strains were grown in whole feather broth medium. Out of 16 proteolytic bacterial isolates, 9 feather degrading isolates were selected from the Parande road side feather waste dumping area. Among these isolates, P3A was selected and classified on the basis of morphological and biochemical analysis. 16S rRNA studies confirmed that the isolated strain was Staphylococcus epidermidis. Staphylococcus epidermidis P3A shows 84% feather degradation and 72 U/ml maximum keratinase activity after 96 hrs. The isolate shows potential use in feather waste disposal methods which are safe and environment friendly.

Construction of simplified microbial consortia to degrade recalcitrant materials based on enrichment and dilution-to-extinction cultures

The capacity of microbes degrading recalcitrant materials has been extensively explored from environmental remediation to industrial applications. Although significant achievements were obtained with single strains, focus is now going toward the use of microbial consortia because of advantages in terms of functional stability and efficiency. While consortia assembly attempts were made from several known single strains, another approach consists in obtaining consortia from complex environmental microbial communities in search for novel microbial species, genes and functions. However, assembling efficient microbial consortia from complex environmental communities is far from trivial due to large diversity and biotic interactions at play. Here we propose a strategy containing enrichment and dilution-to-extinction cultures to construct simplified microbial consortia (SMC) for keratinous waste management, from complex environmental communities. Gradual dilutions were performed from a keratinolytic microbial consortium, and dilution 10−9was selected to construct a SMC library. Further compositional analysis and keratinolytic activity assays demonstrated that microbial consortia were successfully simplified, without impacting their biodegradation capabilities. These SMC possess promising potential for efficient keratinous valorization. More importantly, this reasoning and methodology could be transferred to other topics involving screening for simplified communities for biodegradation, thus considerably broadening its application scope.ImportanceMicrobial consortia have got more and more attention and extensive applications due to their potential advantages. However, a high diversity of microbes is likely to hide uncontrollable risks in practice specific to novel strains and complicated interaction networks. Exploring a convenient and efficient way to construct simplified microbial consortia is able to broaden the applied scope of microbes. This study presents the approach based on enrichment and dilution-to-extinction cultures, which gain abundance microbial consortia including some without losing efficiency from the enriched functional microbial community. The microbial interactions at the strain level were evaluated by using compositional identification and correlation analysis, which contribute to revealing the roles of microbes in the degradation process of recalcitrant materials. Our findings provide a systematic scheme to achieve optimizing microbial consortia for biodegradation from an environmental sample, could be readily applied to a range of recalcitrant materials management from environmental remediation to industrial applications.