Identification of Bacteriocin linocin M18 from Brevibacterium and Related Genera using PCR

Fifty bacterial isolates isolated from dairy product, skin and blood from cancer and kidney failure dialysis patients were identified to twenty two species and the following genera:- Brevibacterium, Corynebacterium, Arthrobacter, Actinomyces, Exiguobacterium, Kocuria, Micrococcus, Rothia, Rhodococcus using a set of phenetic characteristics. Twelve isolates of the different species from the genera Brevibacterium, Arthrobacter, Corynebacterium, Kocuria, Rhodococcus, Rothia were selected and probed for lin gene by polymerase chain reaction. One species Kocuria rhizophila which inhibited most of the tested organisms did not have lin gene in the chromosome, while, the species Corynebacterium glucuronolyticum, Arthrobacter comminsii, Arthrobacter oxydans have the lin gene. Our results found there wide distribution of the structural gene encoding this linocin M18 within coryneform bacteria and also in the genus Kocuria.

Corynebacterium pyruviciproducens sp. nov., a pyruvic acid producer

A coryneform strain, 06-1773OT (=WAL 19168T), derived from a groin abscess sample was characterized using phenotypic and molecular taxonomic methods. Comparative analyses revealed more than 3 % divergence of the 16S rRNA gene sequence and about 10 % divergence of the partial rpoB gene sequence from the type strain of Corynebacterium glucuronolyticum. The strain could also be differentiated from C. glucuronolyticum by a set of phenotypic properties. A DNA–DNA relatedness study between strain WAL 19168T and C. glucuronolyticum CCUG 35055T showed a relatedness value of 13.3 % (13.7 % on repeat analysis). The genotypic and phenotypic data show that the strain merits classification within a novel species of Corynebacterium. We propose the name Corynebacterium pyruviciproducens sp. nov. for the novel species. The type strain is 06-1773OT (=WAL 19168T =CCUG 57046T =ATCC BAA-1742T).