A revision of the New World Euphorbia adiantoides complex (Euphorbiaceae)

The Euphorbia adiantoides complex is here considered to consist of four species. This group is readily distinguished from other New World Euphorbia by the combination of two unusual features: entire styles with capitate stigmas and dichasial bracts with relatively long, filiform stipules. Euphorbia sonorae is reduced to a synonym of Euphorbia adiantoides, a taxon disjunctly distributed between Mexico and western South America. The other species of the complex are all restricted to Mexico. Two of these are described as new: E. zamudioi, an endemic to the Sierra Madre Oriental, and E. breedlovei, which is widespread in central and southern Mexico. A key to distinguish the species is provided, as too are data concerning their morphology, distribution, habitat, phenology, common names, and uses. Phylogenetic analyses were conducted using the nuclear ITS and the chloroplast psbA-trnH regions and including multiple samples of each species. The phylogenetic results are not always congruent with morphology, and of the four species herein recognized, only Euphorbia zamudioi is suggested to form an exclusive, well-supported lineage. This species is nested within E. breedlovei, and two collections of E. breedlovei from central Mexico are more closely related to E. zamudioi than they are to other E. breedlovei from southern Mexico. We hypothesize that E. zamudioi arose through peripatric speciation, in which a northern population of E. breedlovei became reproductively isolated and morphologically differentiated from the remainder of the populations of E. breedlovei.

Peripatric speciation associated with genome expansion and female-biased sex ratios in the moss genus Ceratodon

ABSTRACTPREMISE OF THE STUDYA period of allopatry is widely believed to be essential for the evolution of reproductive isolation. However, strict allopatry may be difficult to achieve in some cosmopolitan, spore-dispersed groups, like mosses. Here we examine the genetic and genome size diversity in Mediterranean populations of the moss Ceratodon purpureus s.l. to evaluate the role of allopatry and ploidy change in population divergence.METHODSWe sampled populations of the genus Ceratodon from mountainous areas and lowlands of the Mediterranean region, and from western and central Europe. We performed phylogenetic and coalescent analyses on sequences from five nuclear introns and a chloroplast locus to reconstruct their evolutionary history. We also estimated the genome size using flow cytometry, employing propidium iodide, and determined their sex using a sex-linked PCR marker.KEY RESULTSTwo well differentiated clades were resolved, discriminating two homogeneous groups: the widespread C. purpureus and a local group mostly restricted to the mountains in southern Spain. The latter also possessed a genome size 25% larger than the widespread C. purpureus, and the samples of this group consist entirely of females. We also found hybrids, and some of them had a genome size equivalent to the sum of the C. purpureus and Spanish genome, suggesting that they arose by allopolyploidy.CONCLUSIONSThese data suggest that a new species of Ceratodon arose via peripatric speciation, potentially involving a genome size change and a strong female-biased sex ratio. The new species has hybridized in the past with C. purpureus.