Multiplexed detection of respiratory pathogens with a portable analyzer in a “raw-sample-in and answer-out” manner

Coronavirus disease 2019 (COVID-19) has emerged, rapidly spread and caused significant morbidity and mortality worldwide. There is an urgent public health need for rapid, sensitive, specific, and on-site diagnostic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In this study, a fully integrated and portable analyzer was developed to detect SARS-CoV-2 from swab samples based on solid-phase nucleic acid extraction and reverse transcription loop-mediated isothermal amplification (RT-LAMP). The swab can be directly inserted into a cassette for multiplexed detection of respiratory pathogens without pre-preparation. The overall detection process, including swab rinsing, magnetic bead-based nucleic acid extraction, and 8-plex real-time RT-LAMP, can be automatically performed in the cassette within 80 min. The functionality of the cassette was validated by detecting the presence of a SARS-CoV-2 pseudovirus and three other respiratory pathogens, i.e., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. The limit of detection (LoD) for the SARS-CoV-2 pseudovirus was 2.5 copies/μL with both primer sets (N gene and ORF1ab gene), and the three bacterial species were successfully detected with an LoD of 2.5 colony-forming units (CFU)/μL in 800 μL of swab rinse. Thus, the analyzer developed in this study has the potential to rapidly detect SARS-CoV-2 and other respiratory pathogens on site in a “raw-sample-in and answer-out” manner.

Can portable analyzers be reliable for biogas characterization?

Anaerobic digestion for treatment of swine wastewater is an attractive alternative, among other aspects, for the generation of biogas. This gas is composed predominantly of methane and can be converted into electrical and thermal energy. However, the knowledge of the biogas composition is of paramount importance, especially regarding the methane content due to its energetic properties. The alternatives for this determination usually require high cost and specialized technicians. Therefore, the search for simple and low cost alternative solutions and techniques can improve the biogas use as an energy source and favor energy sustainability in pig farming. The present study aimed to compare the results of the methane composition of a portable analyzer with that of a Gasboard gas analyzer. The biogas was collected and characterized in a full cycle swine farm from January to December 2019 in the municipality of Teixeiras (MG), Brazil. The methane composition values did not differ statistically for a 5% significance level between the evaluated methods. The use of the portable kit is a simple and low cost alternative in determining the methane content in biogas and can be used reliably.

Economic aspects and managing the work of express laboratory while using portable gas analyzers in resuscitation units

The economic rationale for the choice of a portable analyzer of gases and electrolytes for their use in the resuscitation and intensive care units, as well as implementing the “point of care testing” principle allowed us to optimize the work of the express laboratory, reduce the number of tests, their costs, and increase the quality of research.