Fluorescence of ocular surface in a Covid -19 patient after Favipiravir treatment: a case report

Background Favipiravir is used in treatment of Covid-19 patients. We aimed to share of ocular surface fluorescence in a patient after Favipiravir treatment in this case report. Case presentation A 20-year-old male patient declared no known systemic disease prior to Covid-19. He applied to us with blurry vision and blue light reflection after Covid-19 treatment with Favipiravir. We observed bilateral fluorescence on his eyes and fluorescence of his nails. Biomicroscopic examination was insignificant. Conclusion We investigated the fluorescence of favipiravir tablets under ultraviolet light. Drug demonstrated fluorescence. We recorded the favipiravir fluorescence in-vitro. This appears to be a strong evidence in terms of the linkage between the fluorescence of the ocular surface and favipiravir.

Fluorescence of Ocular Surface in a Covid -19 Patient After Favipiravir Treatment: a Case Report

BackgroundFavipiravir is used in treatment of Covid-19 patients. We aimed in this case report to share of ocular surface fluorescence in a patient after Favipiravir treatment.Case PresentationA 20 years-old male ,with no medical history, applied to us with blurry vision and blue light reflection after Covid-19 treatment with Favipiravir. We observed bilateral fluorescence on his eyes and fluorescence of his nails. Biomicroscopic examination was insignificant.Conclusion We investigated the fluorescence of favipiravir tablets under ultraviolet light. Drug demonstrated fluorescence. We recorded the favipiravir fluorescence independently of the patient. This is strong evidence that the fluorescence of the patient’s ocular surface and favipiravir are linked.

Spectroscopic detection of microbial colonization in Antarctic sandstone

We report infrared reflectance and ultraviolet fluorescence spectra of the surfaces and cleaved side of Beacon Sandstone from Antarctica that harbours a cryptoendolithic microbial community - a photosynthesis-based consortium of algae, lichen and bacteria present a few millimetres below the surface. Chlorophyll absorptions were present in the reflectance spectra of the exposed interior but not on the top or bottom surfaces and their band depths changed < 4% between measurements taken 19 years apart, indicating the stability of the microorganisms when the sample is kept dry. The presence of subsurface organic layers was detected in reflectance at 3.41 μm on the sample's surface. Fluorescence spectra of the cleaved side showed the blue fluorescence peaks expected from chlorophyll but no 0.65–0.80 μm peaks seen in fluorescence measurements of green vegetation. A weak fluorescence signal was detectable at the surface of the sample, presumably due to some light leaking into the subsurface through pores or cracks in the goethite coating the sample's surface. Theoretically, this weak fluorescence signal could possibly be observed in rock surfaces broken by erosion or meteor impacts on Mars. Sandstone outcrops have been reported on Mars and detection of organic layers in sandstones there would be of interest.

Shear-mediated sol-gel transition of regenerated silk allows the formation of Janus-like microgels

Microcapsules and microgels consisting of macromolecular networks have received increasing attention due to their biomedical and pharmaceutical applications. Protein microgels and in particular silk-based microcapsules have desirable properties due to their biocompatibility and lack of toxicity. Typically such structures formed through emulsion templating are spherical in geometry due to interfacial tension. However, approaches to synthesis particles with more complex and non-spherical geometries are sought due to their packing properties and cargo release characteristics. Here, we describe a droplet-microfluidic strategy for generating asymmetric tubular-like microgels from reconstituted silk fibroin; a major component of native silk. It was determined using fluorescence microscopy, that the shear stress within the microchannel promotes surface protein aggregation, resulting in the asymmetric morphology of the microgels. Moreover, the structural transition that the protein undergoes was confirmed using FTIR. Crucially, the core of the microgels remains liquid, while the surface has fully aggregated into a fibrillar network. Additionally, we show that microgel morphology could be controlled by varying the dispersed to continuous phase flow rates, while it was determined that the radius of curvature of the asymmetric microgels is correlated to the wall shear stress. By comparing the surface fluorescence intensity of the microgels as a function of radius of curvature, the effect of the shear stress on the amount of aggregation could be quantified. Finally, the potential use of these asymmetric microgels as carriers of cargo molecules is showcased. As the core of the microgel remains liquid but the shell has gelled, this approach is highly suitable for the storage of bio-active cargo molecules such as antibodies, making such a delivery system attractive in the context of biomedical and pharmaceutical applications.

Shear-Mediated Sol-Gel Transition of Regenerated Silk Allows the Formation of Janus-like Microgels

<div>Microcapsules and microgels consisting of macromolecular networks have received increasing attention due to their biomedical and pharmaceutical applications. Protein microgels and in particular silk-based microcapsules have desirable properties due to their biocompatibility and lack of toxicity. Typically such structures formed through emulsion</div><div>templating are spherical in geometry due to interfacial tension. However, approaches to synthesise particles with more complex and non-spherical geometries are sought due to their packing properties and cargo release characteristics. Here, we describe a droplet-microfluidic strategy for generating asymmetric tubular-like microgels from reconstituted silk fibroin; a major component of native silk. It was determined using fluorescence microscopy, that the shear stress within the microchannel promotes surface protein aggregation, resulting in the asymmetric morphology of the microgels. Moreover, the structural transition that the protein undergoes was confirmed using FTIR. Crucially, the core of the microgels remains liquid, while the surface has fully aggregated into a fibrillar network. Additionally, we show that microgel morphology could be controlled by varying the dispersed to continuous phase flow rates, while it was determined that the radius of curvature of the asymmetric microgels is correlated to the wall shear stress. By comparing the surface fluorescence intensity of the microgels as a function of radius of curvature, the effect of the shear stress on the amount of aggregation could be quantified. Finally, the potential use of these asymmetric microgels as carriers of cargo molecules is showcased.</div><div>As the core of the microgel remains liquid but the shell has gelled, this approach is highly suitable for the storage of bio-active cargo molecules such as antibodies, making such a delivery system attractive in the context of biomedical and pharmaceutical applications.</div>

Shear-Mediated Sol-Gel Transition of Regenerated Silk Allows the Formation of Janus-like Microgels

<div>Microcapsules and microgels consisting of macromolecular networks have received increasing attention due to their biomedical and pharmaceutical applications. Protein microgels and in particular silk-based microcapsules have desirable properties due to their biocompatibility and lack of toxicity. Typically such structures formed through emulsion</div><div>templating are spherical in geometry due to interfacial tension. However, approaches to synthesise particles with more complex and non-spherical geometries are sought due to their packing properties and cargo release characteristics. Here, we describe a droplet-microfluidic strategy for generating asymmetric tubular-like microgels from reconstituted silk fibroin; a major component of native silk. It was determined using fluorescence microscopy, that the shear stress within the microchannel promotes surface protein aggregation, resulting in the asymmetric morphology of the microgels. Moreover, the structural transition that the protein undergoes was confirmed using FTIR. Crucially, the core of the microgels remains liquid, while the surface has fully aggregated into a fibrillar network. Additionally, we show that microgel morphology could be controlled by varying the dispersed to continuous phase flow rates, while it was determined that the radius of curvature of the asymmetric microgels is correlated to the wall shear stress. By comparing the surface fluorescence intensity of the microgels as a function of radius of curvature, the effect of the shear stress on the amount of aggregation could be quantified. Finally, the potential use of these asymmetric microgels as carriers of cargo molecules is showcased.</div><div>As the core of the microgel remains liquid but the shell has gelled, this approach is highly suitable for the storage of bio-active cargo molecules such as antibodies, making such a delivery system attractive in the context of biomedical and pharmaceutical applications.</div>