Fine-Scale Mapping of Mega-Epibenthic Communities and Their Patch Characteristics on Two New Zealand Seamounts

Seamounts are common features of the deep seafloor that are often associated with aggregations of mega-epibenthic fauna, including deep-sea corals and sponges. Globally, many seamounts also host abundant fish stocks, supporting commercial bottom trawl fisheries that impact non-target benthic species through damage and/or removal of these non-target species. However, the effects of bottom trawling on seamount benthic communities, as well as their recovery potential, will vary over the total seamount area because of differences in within seamount habitat and community structure. It is therefore important to understand fine-scale community dynamics, community patch characteristics, and the environmental drivers contributing to these patterns to improve habitat mapping efforts on seamounts and to determine the potential for benthic communities on seamounts to recover from fishing disturbances. Here we analysed the structure and distribution of mega-epibenthic communities on two New Zealand seamounts with different physical environments to determine which environmental variables best correlated with variation in community structure within each seamount. We used the identified environmental variables to predict the distribution of communities beyond the sampled areas, then described the spatial patterns and patch characteristics of the predicted community distributions. We found the environmental variables that best explained variations in community structure differed between the seamounts and at different spatial scales. These differences were reflected in the distribution models: communities on one seamount were predicted to form bands with depth, while on the other seamount communities varied mostly with broadscale aspect and the presence of small pinnacles. The number and size of community patches, inter-patch distances, and patch connectedness were found to differ both within and between seamounts. These types of analyses and results can be used to inform the spatial management of seamount ecosystems.

Leflunomide Loaded Chitosan Nanoparticles for the Preparation of Aliphatic Polyester Based Skin Patches

In the present study, the preparation of controlled-released leflunomide (LFD)-loaded skin patches was evaluated, utilizing the combination of chitosan (CS) nanoparticles (NPs) incorporated into suitable poly(l-lactic acid) (PLLA) or poly(lactic-co-glycolic acid) (PLGA) polyester matrices. Initially, LFD-loaded CS NPs of ~600 nm and a smooth surface were prepared, while strong inter-molecular interactions between the drug and the CS were unraveled. In the following step, the prepared LFD-loaded CS NPs were incorporated into PLLA or PLGA, and thin-film patches were prepared via spin-coating. Analysis of the prepared films showed that the incorporation of the drug-loaded CS NPs resulted in a significant increase in the drug’s release rate and extent as compared to neat LFD-loaded polyester patches (i.e., prepared without the use of CS NPs). In-depth analysis of the prepared formulations showed that the amorphization of the drug within the matrix and the increased wetting properties of the prepared CS NPs were responsible for the improved thin-film patch characteristics.

Scale-dependent effects of geography, host ecology, and host genetics on diversity of a stickleback parasite metacommunity

Many metacommunities are distributed across habitat patches that are themselves aggregated into groups. Perhaps the clearest example of this nested metacommunity structure comes from multi-species parasite assemblages, which occupy individual hosts that are aggregated into host populations. At both spatial scales, we expect parasite community diversity in a given patch (either individual host or population) to depend on patch characteristics that affect colonization rates and species sorting. But, are these patch effects consistent across spatial scales? Or, do different processes govern the distribution of parasite community diversity among individual hosts, versus among host patches? To answer these questions, we document the distribution of parasite richness among host individuals and among populations in a metapopulation of threespine stickleback (Gasterosteus aculeatus). We find some host traits (host size, gape width) are associated with increased parasite richness at both spatial scales. Other patch characteristics affect parasite richness only among individuals (sex), or among populations (lake size, lake area, elevation, and population mean heterozygosity). These results demonstrate that some rules governing parasite richness in this metacommunity are shared across scales, while others are scale-specific.