Effects of essential oils and their combinations added to broiler diets on the mineral contents of some tissues and bone breaking strength

At the end of the experiment, approximately 5 cc of blood was taken from broilers, and the serum was separated by centrifugation at 3000 rpm for 5 min. Serum mineral concentrations were determined directly in an atomic emission spectrometer (AX-ICP, Varian Vista). Subsequently, the tibia was removed from the slaughtered broilers, and the meat and bone marrow were cleaned and dried at 105°C for 24 h. Samples were taken from similar parts of each tibia (middle axis). The mineral content of the tibias was determined with an atomic emission spectrometer (ICP, VISTA AX CCD Simultaneous ICP-AES) after wet decomposition of the tibia in a microwave (Mars Xpress Technology Inside).

Variasi Waktu Kontak Maksimum Adsorben Terhadap Logam Hg

Penelitian mengenai efektivitas kitosan dari cangkang belangkas (Tachypleus Gigas) dalam proses penurunan kadar logam Hg pada limbah cair tambang emas telah dilakukan. Kitosan yang digunakan dibuat dengan memanfaatkan cangkang belangkas melalui proses deproteinisasi dan demineralisasi. Kitosan yang dihasilkan digunakan sebagai adsorben untuk menurunkan kadar Hg dalam limbah cair tambang emas dengan hasilnya dibandingkan dengan kitosan murni. Analisis kadar logam Hg denganmenggunakan induktif ditambah Plasma - Atomic Emission Spectrometer (ICP - AES), dimana diperoleh bahwa adsorben efektif digunakan pada waktu kontak 25 menit dengan hasil penyerapan paling maksimal diantara variasi waktu kontak yang lain. Sedangkan kitosan dari cangkang belangkas hanya mampu menghasilkan penyerapan sebesar 34.46% dan kitosan murni 39.69%.

PSVII-14 Toxic and essential elements in seminal plasma and serum in stallions

The aim of the work was compare the concentration the of toxic and essential elements in the sperm plasma and blood serum of stallions. Sperm plasma and serum were collected from 39 Arabian stallions, aged 5 to 15 years. Concentration of toxic elements [aluminum (Al), arsenic (As), cadmium (Cd), lead (Pb), tin (Sn), mercury (Hg)] and essential elements (boron (B), lithium (Li), nickel (Ni), strontium (Sr) and vanadium (V)) in the sperm plasma and serum were determined by atomic emission and mass spectrometry (AES-ICP and MS-ICP) using an mass spectrometer Elan 9000 (Perkin Elmer, USA) and an atomic emission spectrometer Optima 2000V (Perkin Elmer, USA). It is established that the concentration of toxic and essential elements in the sperm plasma is higher than in serum. The greatest difference between the average concentrations of elements in the sperm plasma and serum was set for Sr - 252.9 times, Hg - 85.1 times and Al - 20.7 times. The minimum difference between the average concentrations in the sperm plasma and serum was set for B - 0.3 times, Li - 1.1 times and As - 1.8 times. For the remaining elements, the difference between the averages was in the range 2.7 - 8.5 times. It can be assumed that a greater amount of toxic and essential elements accumulate in the accessory gonads and enter the sperm during ejaculation. Authors acknowledge financial support from Russian Science Foundation, Grant No: 17-16-01109, development program of Bioresource collections “Cryobank of genetic recourses the All-Russian Research Institute for Horse Breeding.”

Metrological and operational characteristics of the multichannel vacuum atomic-emission spectrometer for educational and industrial laboratories with a MS-200UN spark generator

This work presents the metrological and operational characteristics of the multichannel vacuum atomic-emission spectrometer for educational and industrial laboratories with a MS-200UN spark generator. The spectrometer uses an original optical scheme consisting of two polychromators: the first polychromator is designed to work in the field of vacuum ultraviolet at the wavelength range from 170 to 290 nm; the second polychromator covers the wavelength range from 290 to 410 nm. The multielement semiconductor photodetectors with remote shielding windows offer fast recording of spectra in the vacuum ultraviolet range for simultaneous evaluation of the concentrations of several chemical elements in the samples under study. It is demonstrated that metrological characteristics of the atomic-emission vacuum spectrometer with a MS-200UN spark generator for educational and industrial laboratories meet the requirements of the normative documents standardizing analysis of metals and alloys. The designed spectrometer may be used in educational process and during routine analyses at laboratories of industrial enterprises and other organizations.

Analysis of Heavy Metal Content in Conventional and Herbal Toothpastes Available at Maltese Pharmacies

Although toothpastes are considered as topical cosmetics that are not normally ingested, it is evident that they may contribute to the introduction of heavy metals and xenobiotics through buccal and gastrointestinal absorption. The purpose of this study was to determine the potential presence of metals and polyphenols in conventional, children’s and herbal toothpastes. Metal analysis was conducted by using the Microwave Plasma-Atomic Emission Spectrometer and the total polyphenolic content was determined by using the Folin–Ciocalteu test. Results showed that cadmium and mercury were absent in all toothpastes while zinc and tin exhibited high values. This was because the latter two metals are incorporated as part of the ingredients. In the case of polyphenols, the highest value was obtained in one of the samples from the children’s toothpaste category while the lowest value was obtained from the conventional toothpaste category. Lead and nickel were two other metals that fell outside the limits for EU and US standards. Most of these limits are usually applicable to topical cosmetic products or food products. However, these may not adequately cover oral hygiene products, such as toothpastes.