Tales of schizomid tails: patterns in schizomid flagellum shape from elliptical Fourier analysis

The arachnid order Schizomida is a relatively understudied group of soil-dwelling predators found on all continents except Antarctica. While efforts to understand their biology are growing, there is still much to know about them. A curious aspect of their morphology is the male flagellum, a sexually dimorphic, tail-like structure which differs in shape across the order and functions in their courtship rituals. The flagellar shape is important for taxonomic classification, yet few efforts have been made to examine shape diversity across the group. Using elliptical Fourier analysis, a type of geometric morphometrics based on outline shape, we quantified shape differences across a combined nearly 550 outlines in the dorsal and lateral views, categorizing them based on genus, family, biogeographic realm, and habitat, with special emphasis on Caribbean and Cuban fauna. We tested for allometric relationships, differences in disparity based on locations and sizes in morphospace among these categories, and for clusters of shapes in morphospace. We found multiple differences in all categories despite apparent overlaps in morphospace, evolutionary allometry, and evidence for discrete clusters in some flagellum shapes. This study can serve as a foundation for further study on the evolution, diversification, and taxonomic utility of the male flagellum.

Parallelism in Endocarp Form Sheds Light on Fruit Syndrome Evolution in Viburnum

Abstract— All Viburnum species produce drupes with a hardened endocarp surrounding a single seed. Endocarp form varies greatly within Viburnum, and differences in shape have long been used to distinguish major subclades. Here we trace the evolution of Viburnum endocarp shape using morphometric analyses and phylogenies for 115 Viburnum species. Endocarp measurements were obtained from fruits sampled from herbarium specimens and from field collections, and shapes were analyzed using elliptical Fourier analysis. We infer that the first viburnums had flattened and grooved endocarps. Subsequently, there were multiple losses of grooving in conjunction with shifts to both highly flattened and nearly round endocarps. In several clades the parallel evolution of a derived endocarp shape was accompanied by changes in a suite of other fruit traits, yielding distinctive fruit syndromes likely related to bird dispersal. However, in other clades endocarp shapes similar to the ancestral form have been retained while other fruit traits (color, amount of flesh, nutritional content) have diverged. We quantify cases of parallel evolution in endocarp shape that cut across recognized fruit syndromes such as red, carbohydrate-rich fruits with flattened endocarps or blue, lipid-rich fruits with round endocarps. Our analyses now invite studies of function and the selective factors that have yielded the distinctive suites of fruit and seed traits that distinguish the major Viburnum lineages.

Comparing maxillary first molar crown shape using elliptical Fourier analysis in the Late Neolithic cave burials of Belgium

The Belgian Meuse karstic basin holds more than 200 Late Neolithic collective burials. Four of the largest include Hastière Caverne M, Hastière Trou Garçon C, Sclaigneaux and Bois Madame. The remains from these caves are commingled and fragmentary. However, in situ maxillary molars are well preserved permitting an investigation of molar crown shape within and across sites. Crown outlines from the burials are compared using elliptical Fourier analysis to capture shape distinctions in the relatively numerous first maxillary molars (n = 27). Elliptical Fourier analysis is designed to compare deviations between each shape outline and an idealized ellipse, recorded as amplitudes of the harmonics which are reduced to principal components (PC) scores. We expect individuals from each site will be more similar to one another than to other internments in PC scores, and that the sites will be distributed along PC axes according to differences in chronology and geographic location. Principal components analysis reveals that individuals tend to cluster together based on cave burial as well as time period. Geographic distance only differentiates the final/late Neolithic cave burials. The earliest of the sites, Hastière Caverne M, is distinctive and includes multiple outliers. Hastière Trou Garçon C from earlier in the Late Neolithic does not cluster with Hastière Caverne M as expected. Instead, this cave burial groups with Sclaigneaux, the most geographically distant site but chronologically the closest to Hastière Trou Garçon C. Although the limited sample sizes for each site must be considered, it appears that early farmers of the Belgian Meuse basin exhibited intricate human population dynamics which may have included small, semi-isolated groups early in the Late Neolithic and larger communities with greater contact toward the onset of the northern European Bronze Age.

Assessing the patterns and drivers of shape complexity in the amblypygid pedipalp using elliptical Fourier analysis

Amblypygids are an arachnid order possessing a unique pair of spined pedipalps: appendages that perform in prey capture, courtship and contest. Pedipalp length, hypothesised to be under sexual selection, varies markedly across amblypygid species, and pedipalp spination, thought to reflect selection for function in prey capture, also differs interspecifically. Differences in pedipalp shape between species may indicate that the relative strength of selection for prey capture and sexual selection vary across the group. However, interspecific differences in pedipalp shape have not been quantified, due to difficulties in identifying homologous features. For the first time, we quantify trends in amblypygid pedipalp shape complexity. We use elliptical Fourier analysis to quantify 2D complexity in pedipalp outlines across eleven species and six genera. We find that complexity significantly decreases as pedipalp length increases. This appears to be driven by relative spine length, suggesting that a trade-off exists between pedipalp length and spination. Furthermore, significant female-biased sexual dimorphism in shape complexity is present in the tibial segment of the amblypygid pedipalp. Our results provide novel insights into the drivers of amblypygid pedipalp evolution, and suggest that a functional trade-off between performance in prey capture and other functions under sexual selection exist in this enigmatic structure.

High-throughput gene screen reveals modulators of nuclear shape

Using a high-throughput RNAi screen of epigenetic regulators combined with an automated elliptical Fourier analysis, we identify genes required for the maintenance of nuclear shape in breast epithelial cells.

Identificación de stocks de Engraulis ringens en Chile utilizando morfometría de otolitos

Anchovy (Engraulis ringens) population units were analyzed in three zones off the coast of Chile: 1: Arica-Iquique, 2: Coquimbo and 3: Talcahuano-Valdivia from samples obtaineds during the 2012 spawning season. We used 50 left sagittae otoliths from each zone to perform a morphometric analysis, which included basic measurements, shape indexes and contour analysis (elliptical Fourier analysis). A MANOVA and Tukey multiple comparison analyses, applied on basic measures and shape indexes showed significant differences between zone 3 and zones 1 and 2. A classification by Canonical Discriminant analysis of elliptical Fourier harmonics, indicated significant differences among zones. It is concluded, therefore, that otolith shape analysis could be used to discriminate population units of Engraulis ringens. Better results were achieved using elliptic Fourier coefficients than using only shape indices.