Benchmarking statistical methods for analyzing parent-child dyads in genetic association studies

Genetic association studies of child health outcomes often employ family-based designs. One of the most popular family-based designs is the case-parent trio design that considers the smallest possible nuclear family consisting of two parents and their affected child. This trio design is particularly advantageous for studying relatively rare disorders because it is less prone to type 1 error inflation due to population stratification compared to population-based study designs (e.g., case-control studies). However, obtaining genetic data from both parents is difficult, from a practical perspective, and many large studies predominantly measure genetic variants in mother-child dyads. While some statistical methods for analyzing parent-child dyad data (most commonly involving mother-child pairs) exist, it is not clear if they provide the same advantage as trio methods in protecting against population stratification, or if a specific dyad design (e.g., case-mother dyads vs. case-mother/control-mother dyads) is more advantageous. In this article, we review existing statistical methods for analyzing genome-wide data on dyads and perform extensive simulation experiments to benchmark their type I errors and statistical power under different scenarios. We extend our evaluation to existing methods for analyzing a combination of case-parent trios and dyads together. We apply these methods on genotyped and imputed data from multi-ethnic mother-child pairs only, case-parent trios only or combinations of both dyads and trios from the Gene, Environment Association Studies consortium (GENEVA), where each family was ascertained through a child affected by nonsyndromic cleft lip with or without cleft palate. Results from the GENEVA study corroborate the findings from our simulation experiments. Finally, we provide recommendations for using statistical genetic association methods for dyads.

Peran perempuan dalam dakwah keluarga

The role of women in the family is strong and cannot be separated. Its existence is missed and even needed by the children and husband. Women (in this case mother or wife) have a strong heart relationship with children so that the leadership of women in the family cannot be ignored or avoided. However, there are still people who doubt the ability of women as da'wah leaders in the family because of the many dynamics and challenges of preaching in the family. The physical and psychological closeness of women often causes difficulties for children to be obeyed, especially when the children have bad habits. Therefore, this paper will discuss how the leadership of da'wah for women in families. The results of this study explain that women can become play a role as the manager of the family spirit, sholihah morality education, regulator of meeting daily needs and enterainers of family members.

NAT2 and oral clefts: evaluation of genetic risk and the relative importance of embryo and maternal genotypes

Non-syndromic cleft lip with or without cleft palate (NSCLP) is a congenital malformation that shows the characteristics of a multifactorial pathology. In order to describe the genetic predisposition to this disorder, NAT genes were analyzed with special interest since they codify for N-acetyltransferases, the enzymes responsible for the biotransformation of arylamines, hydrazine drugs and a great number of toxins and carcinogens present in diet, cigarette smoke and the environment. The allelic transmission of NAT2 that determines the slow acetylator phenotype in 174 trios (case-mother/father) from ECLAMC (Latin American Collaborative Study of Congenital Malformations) maternities in Argentina was evaluated. The *4, *5B, *6, and *7 variants by PCR-RFLP were analyzed. A higher risk for the 5B*5B* genotypes (OR=2. 24; p=0.050) was found, at the expense of the cases from Patagonia, without the influence of the maternal genotype.

Examining Mother Teresa’s narrative as prophetic: A case study

This paper examines a rhetorical case - Mother Teresa's narrative - for evidence of prophetic qualities, including social calls to action. Mother Teresa's story is considered through established methods of investigating prophecy, such as themes of announcements of judgment and reason and the Messenger formula. Her rhetoric is also examined through the theoretical lens of Walter Fisher's narrative coherence for evidence of biblical ideals and body language. Her lived experiences are also considered evidence of her prophetic nature. Mother Teresa's narrative is read and better valued as part of a wider context of social action consistent with prophecy.