Co-infection with Angiostrongylus chabaudi and Dirofilaria immitis in a wildcat, Felis silvestris from Romania – a case report

Dirofilaria immitis is a worldwide spread nematode affecting the pulmonary artery and the heart of dogs (rarely reported in cats), especially in areas where the dogs show a high prevalence of infection. Angiostrongylus chabaudi is, in turn, a cardiopulmonary nematode of felids identified in several southern European countries and Germany. Co-infections of Dirofilaria immitis and Angiostrongylus spp. are known only in canids. We report a case of D. immitis and A. chabaudi co-infection in a road-killed wildcat originating from Southeastern Romania. Overall, 17 nematodes were collected from the pulmonary arteries of the wildcat and were morphologically identified as A. chabaudi (2 males and 15 females). Another nematode was collected from the right ventricle and identified as adult male D. immitis. Genomic DNA was extracted from one nematode of each of the two species and a fragment of the cox1 gene was amplified and sequenced. The sequences obtained from the nematodes showed 100% similarity to a sequence of A. chabaudi isolated from Romania (Accession number KU521521) and to various D. immitis sequences from Europe, Asia and Australia (e.g. KT716014, EU159111, AJ537512). This is the first report of A. chabaudi and D. immitis co-infection in a felid, revealing the possibility of similar situations in domestic cats. This requires a more in-depth clinical and laboratory examination of animals with respiratory and cardiac symptoms.

Larval development of Angiostrongylus chabaudi, the causative agent of feline angiostrongylosis, in the snail Cornu aspersum

SUMMARYNematodes of the Angiostrongylidae family, such as Angiostrongylus vasorum and Angiostrongylus cantonensis, may cause potentially life-threatening diseases in several mammal species. Alongside these well-known species, Angiostrongylus chabaudi has been recently found affecting the cardiopulmonary system of domestic and wild cats from Italy, Germany, Greece, Romania and Bulgaria. Nonetheless, significant gaps in the understanding of A. chabaudi epidemiology include the lack of information of species acting as intermediate host and of the morphological description of larval stages. Cornu aspersum (n = 30) land snails were infected with 100 first-stage larvae of A. chabaudi collected from a naturally infected wildcat in Romania. Larvae at different developmental stages were found in 29 out of 30 (96·7%) infected snails and a total of 282 (mean 9·8 ± 3·02 larvae per each specimen) were collected from the gastropods. Here we demonstrate that A. chabaudi develops in snails and report C. aspersum as potential intermediate host for this parasitic nematode. Findings of this study are central to understand the ecological features of feline angiostrongylosis and its epidemiology within paratenic and intermediate hosts.

Novel Molecular Assay for Simultaneous Identification of Neglected Lungworms and Heartworms Affecting Cats

Feline lungworms and heartworms are stimulating the interest of the scientific community due to their clinical impact and apparent geographical expansion. Diagnosis of the infections caused by these nematodes is indeed challenging. This report describes a novel multiplex PCR able to identify simultaneously three species of lungworms (Aelurostrongylus abstrususandTroglostrongylus brevior) and heartworms (Angiostrongylus chabaudi) affecting felids. Epidemiological and clinical perspectives are discussed.