Dual regulation of spine-specific and synapse-to-nucleus signaling by PKCδ during plasticity

The activity-dependent plasticity of synapses is believed to be the cellular basis of learning. These synaptic changes are mediated through the coordination of local biochemical reactions in synapses and changes in gene transcription in the nucleus to modulate neuronal circuits and behavior. The protein kinase C (PKC) family of isozymes has long been established as critical for synaptic plasticity. However, due to a lack of suitable isozyme-specific tools, the role of the novel subfamily of PKC isozymes is largely unknown. Here, through the development of FLIM-FRET activity sensors, we investigate novel PKC isozymes in synaptic plasticity in mouse CA1 pyramidal neurons. We find that PKCδ is activated downstream of TrkB and that the spatiotemporal nature of its activation depends on the plasticity stimulation. In response to single spine plasticity, PKCδ is activated primarily in the stimulated spine and is required for local expression of plasticity. However, in response to multi-spine stimulation, a long-lasting and spreading activation of PKCδ scales with the number of spines stimulated and, by regulating CREB activity, couples spine plasticity to transcription in the nucleus. Thus, PKCδ plays a dual functional role in facilitating synaptic plasticity.

Approaches to management and assessment of the health of military services using information technologies

Introduction. At the present stage of developing domestic health care, active processes of informatization of the clinical diagnostic process are taking place. In most regions of the Russian Federation, various medical information systems are actively exploited in medical organizations, digital medical devices and devices are being introduced, which determines the relevance of the formation of a methodology for the use of information technologies in the health care system of military personnel. Materials and methods. The materials of literary sources, bibliographic databases, regulatory documents, research and development work data, materials of military-scientific support of military-medical information systems have been studied. The historical, literary and structural-logical approach, system analysis methods, comparison, composition, and decomposition were used. Results. This study proposes approaches to managing the health of servicemen and a system of its assessment using information technologies. Within the framework of the entire system of medical support for troops at the sectoral level, the ultimate goal of informatization is to construct a single information space for the medical service of the RF Armed Forces. It should be based on the use of unified personal electronic cards of military personnel, a single protected intradepartmental telecommunication network, and medical information systems for various purposes, depending on the level of a medical organization, unit or subdivision. In the informatization of the medical service, it is necessary to distinguish three primary levels, each of which develops its technologies and means of automation: personal (the level of an individual soldier); the level of medical organizations; the systemic level that determines the activities of the entire medical service. A personal electronic card of a serviceman should be used as an individual carrier of passport and medical information in military medical information systems. To decide on the medical and evacuation purpose of a particular patient at the admission and triage department of a medical company, it is necessary to ensure the formation of a protocol of medical and evacuation measures, which includes information from the electronic medical archive, vital activity sensors and medical care accounting systems. Conclusion. The capabilities of information technologies, vital activity sensors, electronic medical archives and communication channels should ensure the collection of relevant information about an individual patient for the needs of the treatment and diagnostic process, even when military medical organizations are deployed at the stages of medical evacuation. The integration of all information systems in the health care system of servicemen into a single information space must be carried out, taking into account the requirements of regional medical information systems and a unified state information system in the field of health.

Dairy Cows Activity under Heat Stress: A Case Study in Spain

Heat stress plays a role in livestock production in warm climates. Heat stress conditions impair animal welfare and compromise the productive and reproductive performance of dairy cattle. Under heat stress conditions, dairy cattle modify their behavior. Thus, the assessment of behavior alterations can be an indicator of environmental or physiological anomalies. Moreover, precision livestock farming allows for the individual and constant monitoring of animal behavior, arising as a tool to assess animal welfare. The purpose of this study was to evaluate the effect of heat stress on the behavior of dairy cows using activity sensors. The study was carried out in Tinajeros (Albacete, Spain) during the summer of 2020. Activity sensors were installed in 40 cows registering 6 different behaviors. Environmental conditions (temperature and humidity) were also monitored. Hourly data was calculated for both animal behavior and environmental conditions. Temperature and Heat Index (THI) was calculated for each hour. The accumulated THI during the previous 24 h period was determined for each hour, and the hours were statistically classified in quartiles according to the accumulated THI. Two groups were defined as Q4 for no stress and Q1 for heat stress. The results showed that animal behavior was altered under heat stress conditions. Increasing THI produces an increase in general activity, changes in feeding patterns and a decrease in rumination and resting behaviors, which is detrimental to animal welfare. Daily behavioral patterns were also affected. Under heat stress conditions, a reduction in resting behavior during the warmest hours and in rumination during the night was observed. In conclusion, heat stress affected all behaviors recorded as well as the daily patterns of the cows. Precision livestock farming sensors and the modelling of daily patterns were useful tools for monitoring animal behavior and detecting changes due to heat stress.

USE OF ELECTRODERMAL WRISTBANDS TO MEASURE STUDENTS' COGNITIVE ENGAGEMENT IN THE CLASSROOM

A pilot project was conducted to study the feasibility of using electrodermal activity sensors embedded in a watch-like device to measure skin conductivity in real time. In the field of education, it may be interesting to use this technology to assess the students' cognitive engagement in the classroom. A few volunteer students as well as the professor were wearing an Empatica E4 wristband during some class periods where different activities were organized such as lectures, workshops and exams. Monitoring several individuals simultaneously makes possible to compare the collected data among students and between the students and the professor. Also, since the activities were weekly repeated, it was possible to assess to which extent the observed patterns were similar from one group to the other. In brief, the collected data is very difficult to interpret, since some external factors seem to have a significant effect on the measurements. Indeed, discrepancies are observed in the data curves representing the students’ electrodermal activity. Also, the data generated by the professor is quite different from one group to the other, even if he repeated the exact same activities at two different times of the week. It is suggested to improve the understanding of all the phenomena that could affect the electrodermal activity measurements before trying to draw conclusions related to the students’ cognitive engagement in the classroom.

Monitoring Physical Activity with a Wearable Sensor in Patients with COPD during In-Hospital Pulmonary Rehabilitation Program: A Pilot Study

Accelerometers have become a standard method of monitoring physical activity in everyday life by measuring acceleration in one, two, or three axes. These devices provide reliable and objective measurements of the duration and intensity of physical activity. We aimed to investigate whether patients undertake physical activity during non-supervised days during stationary rehabilitation and whether patients adhere to the rigor of 24 h monitoring. The second objective was to analyze the strengths and weaknesses of such kinds of sensors. The research enrolled 13 randomly selected patients, qualified for in-patient, 3 week, high-intensity, 5 times a week pulmonary rehabilitation. The SenseWear armband was used for the assessment of physical activity. Participants wore the device 24 h a day for the next 4 days (Friday–Monday). The analysis of the number of steps per day, the time spent lying as well as undertaking moderate or vigorous physical activity (>3 metabolic equivalents of task (METs)), and the energy expenditure expressed in kcal showed no statistically significant difference between the training days and the days off. It seems beneficial to use available physical activity sensors in patients with chronic obstructive pulmonary disease (COPD); measurable parameters provide feedback that may increase the patient’s motivation to be active to achieve health benefits.

Brain endothelial cell TRPA1 channels initiate neurovascular coupling

Cerebral blood flow is dynamically regulated by neurovascular coupling to meet the dynamic metabolic demands of the brain. We hypothesized that TRPA1 channels in capillary endothelial cells are stimulated by neuronal activity and instigate a propagating retrograde signal that dilates upstream parenchymal arterioles to initiate functional hyperemia. We find that activation of TRPA1 in capillary beds and post-arteriole transitional segments with mural cell coverage initiates retrograde signals that dilate upstream arterioles. These signals exhibit a unique mode of biphasic propagation. Slow, short-range intercellular Ca2+ signals in the capillary network are converted to rapid electrical signals in transitional segments that propagate to and dilate upstream arterioles. We further demonstrate that TRPA1 is necessary for functional hyperemia and neurovascular coupling within the somatosensory cortex of mice in vivo. These data establish endothelial cell TRPA1 channels as neuronal activity sensors that initiate microvascular vasodilatory responses to redirect blood to regions of metabolic demand.

Behavioural and physiological responses of individually housed dairy calves to change in milk feeding frequency at different ages

This study aimed to use a range of non-invasive monitoring technologies to investigate the behavioural and physiological responses of individually housed dairy calves to age at change in milk replacer (MR) feeding frequency. Forty-eight Holstein Friesian calves were individually penned and fed MR (625 g/d) as solids in one of three feeding regimes: (i) once-a-day feeding commencing at age 14 d (OAD14), (ii) once-a-day feeding commencing at age 28 d (OAD28) and (iii) twice-a-day feeding (TAD). Several behavioural (automatic activity sensors), physiological (infrared [IR] thermography and heart rate variability [HRV]) and haematological indicators were used to examine calf responses. Reduction in milk feeding frequency at 14 or 28 d of age increased daily concentrate intakes and drinking water consumption throughout the pre-wean period. Calf lying behaviour was unaffected by reduction in milk feeding frequency; however, TAD calves recorded a significant decrease in total daily lying time during the post-wean period compared with OAD28s. There was no effect of treatment on IR eye or rectal temperature throughout the experiment; however, there was an effect of age, with IR temperature decreasing as calf age increased. OAD14 calves tended to have decreased HRV at days 14 and 16, which is suggestive of an increased stress load. The findings suggest that under high levels of animal husbandry and whilst maintaining the same amount of milk powder/d (625 g/d), reduction in milk feeding frequency from twice to once daily at 28 d can occur without significant impact to behavioural, performance and physiological parameters assessed here.