wnk kinase
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Cell Reports ◽  
2021 ◽  
Vol 36 (3) ◽  
pp. 109416
Author(s):  
Paula Schiapparelli ◽  
Natasha L. Pirman ◽  
Kyle Mohler ◽  
Pierre A. Miranda-Herrera ◽  
Natanael Zarco ◽  
...  

2021 ◽  
Vol 118 (23) ◽  
pp. e2025013118
Author(s):  
Selma A. Serra ◽  
Predrag Stojakovic ◽  
Ramon Amat ◽  
Fanny Rubio-Moscardo ◽  
Pablo Latorre ◽  
...  

Regulation of cell volume is essential for tissue homeostasis and cell viability. In response to hypertonic stress, cells need rapid electrolyte influx to compensate water loss and to prevent cell death in a process known as regulatory volume increase (RVI). However, the molecular component able to trigger such a process was unknown to date. Using a genome-wide CRISPR/Cas9 screen, we identified LRRC8A, which encodes a chloride channel subunit, as the gene most associated with cell survival under hypertonic conditions. Hypertonicity activates the p38 stress-activated protein kinase pathway and its downstream MSK1 kinase, which phosphorylates and activates LRRC8A. LRRC8A-mediated Cl− efflux facilitates activation of the with-no-lysine (WNK) kinase pathway, which in turn, promotes electrolyte influx via Na+/K+/2Cl− cotransporter (NKCC) and RVI under hypertonic stress. LRRC8A-S217A mutation impairs channel activation by MSK1, resulting in reduced RVI and cell survival. In summary, LRRC8A is key to bidirectional osmotic stress responses and cell survival under hypertonic conditions.


2020 ◽  
Vol 30 (17) ◽  
pp. 127408
Author(s):  
Shinya Fujii ◽  
Eriko Kikuchi ◽  
Yuko Watanabe ◽  
Honoka Suzuyama ◽  
Mari Ishigami-Yuasa ◽  
...  

Genes ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 986 ◽  
Author(s):  
Holly Mabillard ◽  
John A. Sayer

Gordon syndrome is a rare inherited monogenic form of hypertension, which is associated with hyperkalaemia and metabolic acidosis. Since the recognition of this predominantly autosomal dominant condition in the 1960s, the study of families with Gordon syndrome has revealed four genes WNK1, WNK4, KLHL3, and CUL3 to be implicated in its pathogenesis after a phenotype–genotype correlation was realised. The encoded proteins Kelch-like 3 and Cullin 3 interact to form a ring-like complex to ubiquitinate WNK-kinase 4, which, in normal circumstances, interacts with the sodium chloride co-symporter (NCC), the epithelial sodium channel (ENaC), and the renal outer medullary potassium channel (ROMK) in an inhibitory manner to maintain normokalaemia and normotension. WNK-kinase 1 has an inhibitory action on WNK-kinase 4. Mutations in WNK1, WNK4, KLHL3, and CUL3 all result in the accumulation of WNK-kinase 4 and subsequent hypertension, hyperkalaemia, and metabolic acidosis. This review explains the clinical aspects, disease mechanisms, and molecular genetics of Gordon syndrome.


2019 ◽  
Vol 317 (1) ◽  
pp. C20-C30 ◽  
Author(s):  
Andrée-Anne Marcoux ◽  
Samira Slimani ◽  
Laurence E. Tremblay ◽  
Rachelle Frenette-Cotton ◽  
Alexandre P. Garneau ◽  
...  

Na+-K+-Cl− cotransporter type 2 (NKCC2) is confined to the apical membrane of the thick ascending limb of Henle, where it reabsorbs a substantial fraction of the ultrafiltered NaCl load. It is expressed along this nephron segment as three main splice variants (called NKCC2A, NKCC2B, and NKCC2F) that differ in residue composition along their second transmembrane domain and first intracellular cytosolic connecting segment (CS2). NKCC2 is known to be activated by cell shrinkage and intracellular [Cl−] reduction. Although the with no lysine (WNK) kinases could play a role in this response, the mechanisms involved are ill defined, and the possibility of variant-specific responses has not been tested thus far. In this study, we have used the Xenopus laevis oocyte expression system to gain further insight in these regards. We have found for the first time that cell shrinkage could stimulate NKCC2A- and NKCC2B-mediated ion transport by increasing carrier abundance at the cell surface and that this response was achieved (at least in part) by the enzymatic function of a WNK kinase. Interestingly, we have also found that the activity and cell surface abundance of NKCC2F were less affected by cell shrinkage compared with the other variants and that ion transport by certain variants could be stimulated through WNK kinase expression in the absence of carrier redistribution. Taken together, these results suggest that the WNK kinase-dependent pathway can affect both the trafficking as well as intrinsic activity of NKCC2 and that CS2 plays an important role in carrier regulation.


2018 ◽  
Vol 315 (3) ◽  
pp. F734-F745 ◽  
Author(s):  
Eduardo R. Argaiz ◽  
Maria Chavez-Canales ◽  
Mauricio Ostrosky-Frid ◽  
Alejandro Rodríguez-Gama ◽  
Norma Vázquez ◽  
...  

Familial hyperkalemic hypertension (FHHt) can be mainly attributed to increased activity of the renal Na+:Cl− cotransporter (NCC), which is caused by altered expression and regulation of the with-no-lysine (K) 1 (WNK1) or WNK4 kinases. The WNK1 gene gives rise to a kidney-specific isoform that lacks the kinase domain (KS-WNK1), the expression of which occurs primarily in the distal convoluted tubule. The role played by KS-WNK1 in the modulation of the WNK/STE20-proline-alanine rich kinase (SPAK)/NCC pathway remains elusive. In the present study, we assessed the effect of human KS-WNK1 on NCC activity and on the WNK4-SPAK pathway. Microinjection of oocytes with human KS-WNK1 cRNA induces remarkable activation and phosphorylation of SPAK and NCC. The effect of KS-WNK1 was abrogated by eliminating a WNK-WNK-interacting domain and by a specific WNK inhibitor, WNK463, indicating that the activation of SPAK/NCC by KS-WNK1 is due to interaction with another WNK kinase. Under control conditions in oocytes, the activating serine 335 of the WNK4 T loop is not phosphorylated. In contrast, this serine becomes phosphorylated when the intracellular chloride concentration ([Cl−]i) is reduced or when KS-WNK1 is coexpressed with WNK4. KS-WNK1-mediated activation of WNK4 is not due to a decrease of the [Cl−]i. Coimmunoprecipitation analysis revealed that KS-WNK1 and WNK4 interact with each other and that WNK4 becomes autophosphorylated at serine 335 when it is associated with KS-WNK1. Together, these observations suggest that WNK4 becomes active in the presence of KS-WNK1, despite a constant [Cl−]i.


2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Lubika J. Nkashama ◽  
Ankita Roy ◽  
Rolando Carrisoza‐Gaytan ◽  
Evan C. Ray ◽  
Allison Marciszyn ◽  
...  

2017 ◽  
Vol 60 (16) ◽  
pp. 7099-7107 ◽  
Author(s):  
Ken Yamada ◽  
Julian Levell ◽  
Taeyong Yoon ◽  
Darcy Kohls ◽  
David Yowe ◽  
...  
Keyword(s):  

2017 ◽  
Vol 25 (2) ◽  
pp. 285-299 ◽  
Author(s):  
Masoud Shekarabi ◽  
Jinwei Zhang ◽  
Arjun R. Khanna ◽  
David H. Ellison ◽  
Eric Delpire ◽  
...  

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