UTILIZATION OF ISOLATED THERAPEUTIC BACTERIA IN TOPICAL OINTMENT FOR INFECTION TREATMENT

This study includes the using therapeutic bacteria isolate in topical ointment for bacterial vaginosis treatment and to compare it with metronidazole ointment that use in a clinical trial under medical supervision. Four active Lactobacillus probiotics bacteria were cultured in %12 skim milk , three of these bacteria were commercial types and one Iraqi local isolate , the total bacterial count of probiotics bacteria were around ≥ 1012 cfu/ml . %10 of individually culture free cells filtrate were added to Muller Hinton broth with secretions of patients infected with bacterial vaginosis "BV", %50 of natural organic components and %50 of local isolate Lactobacillus plantarum cultured in skim milk were mixed to prepare the natural ointment. Eighty-nine BV volunteers were divided into two group, first was 43 volunteers used probiotic ointment, the second group was 46 volunteers used metronidazole ointment "as control group", both groups were under medical observation for two weeks. The results shown that Iraqi local isolate Lb . plantarum gave best inhibition in vitro, according to Amsel criteria the rate of healing with Lb . plantarum ointment group was %97.87, it was higher than metronidazole ointment %91.30 and the healed cases numbers were increased at the day 7 and 14,it has been noticed that the results were positive based on vaginal secretions, and whiff test, clue cell and the pH was above 4.5 shown significant progress. According to Nugent criteria Lb . plantarum ointment gave higher healing percentage which was %95.35.

Comparison of the Vaginal Cytological and Microbiological Results in the Detection of Normal Microflora of Pregnant

The aim of this study was to carry out a cytological and microbiological comparative investigation of vaginal microflora in pregnant ewes. The subjects for the study comprised of 39 healthy curly fleeced breed ewes (n=39), approximately 3 years old, at 2-4 months of pregnancy. Two vaginal samples were taken for cytological and microbiological examinations from each ewe in a sterile manner. Hemacolor® was used in cytological examination, while microbiological analysis were completed by conventional techniques. In cytological examination, slides were evaluated to detect lactobacilli, other bacteria, “clue cell” formation and presence of neutrophils. Microbiological investigation was carried out to detect possible pathogens. Cytological results compatible with bacterial vaginosis were obtained in 10 cases. Microbiologically, single type bacteria in 27 cases and more than one bacterium in 12 cases were isolated. The most common isolated pathogen was Escherichia coli. Comparing the cytological and microbiological results, 7 out of 27 cases were compatible with the bacterial vaginosis. In 3 cases of bacterial vaginosis non-pathogenic agents were revealed. In conclusion, it was proven that utilising the cytological examination provides more reliable results for detection of normal vaginal microflora of pregnant ewes.

Profil vaginosis bakterial di Poliklinik Kulit dan Kelamin RSUP Prof. Dr. R. D. Kandou Manado periode Januari 2011-Desember 2015

Bacterial vaginosis (BV) is an abnormal situation in the vaginal ecosystem characterized by Lactobacillus as normal vaginal flora was replaced by a high concentration of anaerobic bacteria, especially Bacteroides sp., Mobilluncus sp., Gardnerella vaginalis, and Mycoplasma hominis. This study was aimed to obtain the profile of bacterial vaginosis in patients treated at the Polyclinic of Dermatovenereology Prof. Dr. R. D. Kandou Hospita; Manado from January 2011 to December 2015. This was a retrospective study using medical records of new BV patients including basic characteristics of patients, such as age, marital status, job, and treatment. The results showed that the number of patients with bacterial vaginosis during the period January 2011-Desember 2015 at the Polyclinic Hospital Dermatology Prof. Dr. R.D. Kandou Manado were as many as 117 patients. The group most affected by BV were at the age of 25-44 years in 61 patients (52%); job as housewife in 42 patients (35%); married in 93 patients (80%); social factor as the precipitating factors in 61 patients (52%). Bacterial vaginosis without concomitant diseases were found in 96 patients (82%), followed by comorbidities BV + vulvovagina candidiasis in 18 patients (15%). The most common therapy in those patients was metronidazole 2x500 mg for 7 days in 111 patients (95%). Conclusion: New cases of bacterial vaginosis were increase in 2011-2013 but they decreaased in 2014-2015.Keywords: bacterial vaginosis, vaginal discharge, bad odor, clue cell Abstrak: Vaginosis bakterial (VB) adalah suatu keadaan abnormal pada ekosistem vagina yang ditandai adanya konsentrasi Lactobacillus sebagai flora normal vagina digantikan oleh konsentrasi tinggi bakteri anaerob, terutama Bacteroides sp., Mobilluncus sp., Gardnerella vaginalis, dan Mycoplasma hominis. Penelitian ini bertujuan untuk mengetahui profil vaginosis bakterial pada penderita yang berkunjung dan berobat di Poliklinik Kulit dan Kelamin RSUP Prof. Kandou Manado periode Januari 2011- Desember 2015. Jenis penelitian ialah retrospektif menggunakan rekam medik kasus baru VB yang meliputi data dasar seperti umur,status perkawinan, pekerjaan dan terapinya. Hasail penelitian mendapatkan jumlah penderita vaginosis bakterial selama periode Januari 2011 - Desember 2015 sebanyak 117 pasien. Kelompok paling banyak terkena vaginosis bakterial ialah umur 25-44 tahun sebanyak 61 orang (52%); pekerjaan Ibu Rumah Tangga (IRT) sebanyak 42 pasien (35%); sudah menikah sebanyak 93 orang (80%); dengan faktor sosial sebagai pencetus sebanyak 61 pasien (52%). Penyakit vaginosis bakterial tanpa penyerta didapatkan 96 pasien (82%) dan diikuti oleh penyakit penyerta VB+KVV yaitu sebanyak 18 pasien (15%). Jenis obat/terapi yang paling sering digunakan pada pasien vaginosis bakterial ialah metronidazole 2x500 mg selama 7 hari sebanyak 111 pasien (95%). Simpulan: Gambaran umum kasus baru Vaginosis bakterial menunjukkan terjadi peningkatan jumlah pasien pada tahun 2011-2013 dan pada tahun 2014-2015 terjadi penurunan Kata kunci: vaginosis bakterial, keputihan, bau, clue cell