Long-term patterns of an interconnected core marine microbiota

ABSTRACTBackgroundOcean microbes constitute ∼70% of the marine biomass, are responsible for ∼50% of the Earth’s primary production, and are crucial for global biogeochemical cycles. Marine microbiotas include core taxa that are usually key for ecosystem function. Despite their importance, core marine microbes are relatively unknown, which reflects the lack of consensus on how to identify them. So far, most core microbiotas have been defined based on species occurrence and abundance. Yet, species interactions are also important to identify core microbes, as communities include interacting species. Here, we investigate interconnected bacteria and small protists of the core pelagic microbiota populating a long-term marine-coastal observatory in the Mediterranean Sea over a decade.ResultsCore microbes were defined as those present in >30% of the monthly samples over 10 years, with the strongest associations. The core microbiota included 259 Operational Taxonomic Units (OTUs) including 182 bacteria, 77 protists, and 1,411 strong and mostly positive (∼95%) associations. Core bacteria tended to be associated with other bacteria, while core protists tended to be associated with bacteria. The richness and abundance of core OTUs varied annually, decreasing in stratified warmers waters and increasing in colder mixed waters. Most core OTUs had a preference for one season, mostly winter, which featured subnetworks with the highest connectivity. Groups of highly associated taxa tended to include protists and bacteria with predominance in the same season, particularly winter. A group of 13 highly-connected hub-OTUs, with potentially important ecological roles dominated in winter and spring. Similarly, 18 connector OTUs with a low degree but high centrality were mostly associated with summer or autumn and may represent transitions between seasonal communities.ConclusionsWe found a relatively small and dynamic interconnected core microbiota in a model temperate marine-coastal site, with potential interactions being more deterministic in winter than in other seasons. These core microbes would be essential for the functioning of this ecosystem over the year. Other non-core taxa may also carry out important functions but would be redundant and non-essential. Our work contributes toth e understanding of the dynamics and potential interactions of core microbes possibly sustaining ocean ecosystem function.

A Control System For Constrained Wave-Powered Oceanographic Buoys

Wave energy can be used to power oceanographic buoys. A new switching control strategy is developed in this paper for a two-body heaving wave energy converter that is composed of a floating cylinder and two rigidly connected submerged hemispheres. This control strategy is designed to prevent excessive displacement of the floating buoy that may occur due to the actuator force. This control strategy switches the control between a multi-resonant controller and a nonlinear damping controller, depending on the state of the system, to account for displacement constraints. This control strategy is developed using a one-degree-of-freedom dynamic model for the relative motion of the two bodies. Estimation of the relative motion, needed for feedback control, is carried out using a Kalman filter. Numerical simulations are conducted to select the proper mooring stiffness. The controller is tested with stochastic models of irregular waves in this paper. The performance of the controller with different sea states is discussed. Annual power production using this control strategy is presented based on real data in 2015 published by Martha's Vineyard Coastal Observatory.

Reconfigurable Power Converter for Constant Current Underwater Observatory

A constant current (CC) underwater observatory employing the shunt method to provide constant voltage (CV) power for external loads is favored in occasions where shunt-fault tolerance is required. However, low efficiency of CC to CV conversion with the shunt method limits its application, especially in scenarios of varying loads. In this paper, a highly reliable and stable CC/CV converter with better efficiency is introduced based on the proposed novel active soft bypass (ASB) technology and the proposed novel priority-based power management strategy (PPMS). The ASB technology is a method that employs switches and a special control sequence which greatly depresses the large voltage transient presenting on the input side when trying hard bypass redundant modules, and the PPMS makes the system easy to monitor and ensures the absolute reliability of ASB technology. The theoretical study of this novel reconfigurable CC/CV converter and validation experiments on a prototype are carried out, with results showing great improvement in the performance. In addition, the proposed reconfigurable power converter is applied to a coastal observatory in the East China Sea.

Relationship between shell integrity of pelagic gastropods and carbonate chemistry parameters at a Scottish Coastal Observatory monitoring site

Ocean acidification (OA), the anthropogenic carbon dioxide-induced changes in seawater carbonate chemistry, is likely to have a significant impact on calcifying plankton. Most planktonic studies on OA are based on “one-off” cruises focused on offshore areas while observations from inshore waters are scarce. This study presents the first analysis on the shell integrity of pelagic gastropods (holoplanktonic pteropods and planktonic larvae of otherwise benthic species) at the Scottish Coastal Observatory monitoring site at Stonehaven on the east coast of Scotland. The shell integrity of archived pelagic gastropods specimens from 2011 to 2013 was examined using Scanning Electron Microscopy and the relationship with OA (pH and aragonite saturation, Ωarg) and other environmental parameters was investigated. Evidence of shell dissolution was detected in all analysed taxa even though the seawater was supersaturated with respect to aragonite. The shell condition matched the temporal pattern observed in Ωarg, with higher proportion of dissolution associated with decreasing Ωarg, suggesting that the seasonality component of carbonate chemistry might affect the shell integrity of pelagic gastropods. The proportion of shell dissolution differed significantly between larvae and adult stages of pteropods, supporting the hypothesis that early-life stages would be more vulnerable to OA-induced changes. Our data also suggest that sensitivity to OA may differ even between closely related taxonomic groups. The strong interannual variability revealed by the year-to-year shell dissolution and Ωarg illustrates the difficulty in assessing the plankton response to OA in the field and the value of time series studies.

Diachronic UAV Photogrammetry of a Sandy Beach in Brittany (France) for a Long-Term Coastal Observatory

In the dual context of coastal hazard intensification and the growing number of stakes exposed to these hazards, coastal observatories are in demand to provide a structured framework dedicated to long-term monitoring. This article describes the drone-based photogrammetry monitoring performed since 2006 on Porsmilin Beach (Brittany, France) in the framework of the DYNALIT (Littoral and Coastline Dynamics) observatory, focusing on data quality and the consistency of long-term time series under the influence of multiple technological evolutions: Unmanned Aerial Vehicles (UAV) platforms with the arrival of electric multirotor drones, processing tools with the development of structure-from-motion (SfM) photogrammetry, and operational modes of survey. A study case is presented to show the potential of UAV monitoring to study storm impacts and beach resilience. The relevance of high-accuracy monitoring is also highlighted. With the current method, an accuracy of 3 cm can be achieved on the digital elevation model (DEM) and the orthophotograph. The question of the representativity and frequency of DEM time points is raised.