PSVII-18 Role of azoximer bromid in functional system «mother-fetus» in cows

The aim was to study efficiency of Azoxivet (azoximer bromid) for prevention of cows’ postnatal diseases and indirect influence on calves’ growth. Groups of pregnant Holstein cows (> 6000 kg per lactation) were formed (G1,G2,G3). Cows of G1 (n = 20) received Azoxivet intramuscularly once at 7 days prior to expected date of calving at a dose 24 mg. G2 (n = 20) - 24 mg at 14 and 7 days prior to expected date of calving. G3 (n = 20) was control. Single application of Azoxivet at 7 days prior to calving doesn’t lead to lowering trouble of postnatal endometritis: this disease was registered at 60% cows of G1 and 65% cows of G3. In G2, endometritis was recorded only at 40% of animals. The density of the colostrum in of G1 and G2 was varied: 1060.0±3.1-1063.0±2.9 kg/m3, which exceeded the indicator of the G3 by 13–16 kg/m3 (P < 0.05). There was increased amount of whey proteins in colostrum obtained from cows of G1 and G2: the differences were 6.4% and 19.2% (P < 0.05). In the G3 it was 220.5 ± 12.6 g/l. The amount of total immunoglobulins in the colostrum in G1 was 159.7 ± 9.6 g/l, in G2 - 180.6 ± 4.0 g/l, that was higher than in the G 3 by 35.0% (P < 0.05). The incidence of calves received from cows of G1 and G2 was lower than 15%, than G3, and the safety to the 2-month age was 90–95% against 85% in G3. The average alive mass of 1 calf in G3 was 77.5±1.6 kg that was lower than in G1 by 5.7% (P < 0.05), in G2 – by 9.6% (P < 0.01). The mediated immune-correction with Azoxivet through the system of fetus-mother allows to reduce incidence, to increase viability of calves in the first 2 months of life.

Effect of a complex therapy including a nonspecific immune correction in pregnant women with type 1 diabetes mellitus on the health status of their children

Цель. Влияние комплексной терапии с включением неспецифической иммунокоррекции (ингаляции гепарина в гестационном периоде у женщин с сахарным диабетом 1-го типа (СД1) с установленной гиперпродукцией аутоантител к инсулину и его рецепторам), на здоровье рожденных ими детей. Материалы и методы. Проведен сравнительный анализ нервно-психического развития, соматического состояния и иммунологических особенностей у детей от рождения до 3 лет от матерей с СД1, получавших традиционную терапию (основная группа, n = 101) и детей от матерей с СД1, получавших во время беременности в составе комплексной терапии ингаляции гепарином (экспериментальная группа, n = 40). Контрольную группу (n = 30) составили дети от соматически здоровых матерей без инфекционной и эндокринной патологии. Результаты. При анализе частоты регистрируемой у детей в период новорожденности патологии ЦНС установлено увеличение данного показателя по сравнению с контролем обеих группах детей, рождённых от матерей с СД1. Однако по результатам балльной оценки (методика «ГНОМ») выявлено, что в экспериментальной группе было достоверно больше здоровых детей и, наоборот, меньше детей с патологией нервной системы, нуждающихся в лечении. При этом что уровень антител к инсулину у детей экспериментальной группы в возрасте 1 год оказался значимо ниже (р < 0,05), чем в основной группе. Заключение. Своевременное применение неспецифической иммунокоррекции в период беременности в составе комплексной терапии беременных женщин с СД1 позволяет нормализовать уровень органоспецифических аутоантител, улучшить клиническое состояние новорожденных детей и прогноз их последующего неврологического развития. Aim. To study the effect of a complex therapy including a nonspecific immune correction (heparin inhalation in the gestational period of women with type 1 diabetes mellitus (DM1) with documented overproduction of insulin and insulin receptor autoantibodies) on the health of their children. Methods. A comparative analysis of neuropsychic development, somatic status, and immunological features was performed in children from birth to three years born by mothers with DM1 who received a standard complex therapy (main group, n = 101) and children from mothers with DM1 who received heparin inhalation as a part of the complex therapy during pregnancy (study group, n = 40). The control group (n = 30) consisted of children from somatically healthy mothers without infectious and endocrine pathology. Results. Analysis of the incidence of CNS pathology observed in newborns during the neonatal period showed an increase in this index in both groups of children born from mothers with DM1 compared to the control. However, according to the results of scoring (GNOM method) the number of healthy children was significantly greater whereas the number of children with CNS pathology who needed treatment was significantly less. Moreover, the level of insulin antibodies was significantly lower (p <0.05) in children of the experimental group at the age of one year than in the main group. Conclusion. The timely use of nonspecific immune correction as a part of the complex therapy of pregnant women with DM1 allows to normalize the level of organ-specific autoantibodies, improve both the clinical condition of newborns and the prediction for their subsequent neurological development.

Therapeutic Apheresis in Oncology

Background: Given the steady growth of oncologic diseases incidence worldwide, their treatment still remains not effective enough. The used methods of radio- and chemotherapy are associated with severe intoxication, underlying thanatogenesis. Besides, the tumor process is also associated with increasing intoxication. Objective: To study pathogenesis of tumor endotoxemia, radio- and chemotherapy complications, and to discover possibilities to use extracorporeal methods of detoxification and immune correction in treatment of malignant tumors. Method: To review the world literature concerning tumor conditions treatment complications and methods of their correction; to use own methods of extracorporeal detoxification and immune correction. Results: WHO reports present rather discouraging findings about a significant increase of patients with malignant tumors for the last 30 years and remaining rather high level of death rate. Using plasmapheresis weekly during the course of radiotherapy for pancreatic cancer was able to prevent a lot of complications and continue the treatment. There is a report about using plasmapheresis with lymphocytes isolation ant their incubation with roncoleukin (IL-2) in patients with small-cell carcinoma of the lungs. They show a significant increase of IL-2, TNFα, and interferon serum concentration in the patients, and their survival rare increased from 0.5 to 2-2.5 years. Conclusion: Extracorporeal detoxification and immune correction can help eliminate endotoxemia and improve the treatment outcome. At the same time, higher doses of chemotherapeutic agents can be used.

The actual problems of immune therapy of pyo-inflammatory diseases

The article considers various clinical models of such pyoinflammatory diseases as deep pyoderma, exacerbation of chronic salpingo-oophoritis, chronic pyelonephritis, implemented to establish determining effect of pathogenesis and possibly basic treatment of pathologic processes on character, intensity of immunopathology, effectiveness and mechanisms of action of pharmacological immune correction.