strong hybridization signal
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Genome ◽  
2010 ◽  
Vol 53 (8) ◽  
pp. 594-598 ◽  
Author(s):  
Alicia López ◽  
Aveliano Fernández ◽  
Lidia Poggio

Subseries Turnera comprises a polyploid complex with ploidy levels ranging from diploid (2n = 2x = 10) to octoploid (2n = 8x = 40). The use of fluorescent in situ hybridization greatly improved the knowledge of the karyotypes of Turnera species by detecting and mapping rDNA sites. Interspecific variability in the number of sites was detected, but not in correlation with the ploidy level. A chromosome pair with a strong hybridization signal was always visible and this signal corresponded to the secondary constriction detectable by conventional techniques. Genomic in situ hybridization experiments combined with information on meiotic pairing in species and interspecific hybrids revealed that homologies detected by molecular analysis are greater than those detected by chromosome pairing. This suggests that the formation of the allopolyploids could involve species more closely related than previously assumed. Despite the molecular affinity among the genomes, the meiotic pairing is probably controlled by specific genes that restrict homeologous pairing in polyploids.


2005 ◽  
Vol 17 (2) ◽  
pp. 176-178 ◽  
Author(s):  
Kwonil Jung ◽  
Chanhee Chae

This report describes the first diagnosis of swine influenza H1N2 virus infection in growing pigs in Korea by virus isolation, reverse transcription–polymerase chain reaction (RT-PCR), histopathology, and in situ hybridization. The subtype of swine influenza virus isolates was determined to be H1N2 by RT-PCR. The most consistent and predominant histological feature was bronchointerstitial pneumonia. Lung tissues from these pigs were hybridized with the nonradioactive digoxigenin-labeled complementary DNA (cDNA) probe from H1 HA and N2 NA genes but not from H3 HA and N1 NA genes. A strong hybridization signal was seen in bronchial- and bronchiolar-lining epithelial cells and in alveolar and interstitial macrophages.


1998 ◽  
Vol 46 (10) ◽  
pp. 1151-1160 ◽  
Author(s):  
Kazuto Mino ◽  
Jun Watanabe ◽  
Shinsuke Kanamura

The effects of an inducer on expression of cytochrome P-450 (P-450) isoforms induced antecedently by another inducer are unknown. Thus, we examined the amount of phenobarbital (PB)-inducible P-450 isoforms (P-450 2B1/2B2) in hepatocytes from rats injected first with PB and then with 3-methylcholanthrene (MC) (PB+MC-treated animals) by quantitative immunohistochemistry. In addition, expression of P-450 2B2 mRNA was examined by in situ hybridization. In PB-treated animals, P-450 2B1/2B2 content increased in perivenular and midzonal hepatocytes. In PB+MC-treated animals, however, the PB-induced increase in 2B1/2B2 content was suppressed in perivenular hepatocytes but promoted in midzonal hepatocytes. The hybridization signal for P-450 2B2 mRNA appeared almost exclusively in perivenular hepatocytes after 24 hr of PB injection and disappeared after 48 hr of injection. In PB+MC-treated animals, however, strong hybridization signal was observed in midzonal and perivenular hepatocytes after 48 hr of PB injection. The promotion of the increase in P-450 2B1/2B2 content in midzonal hepatocytes in PB+MC-treated animals probably corresponds to the strong hybridization signal, whereas there appeared to be a divergence between the intensity of the signal and the content in perivenular hepatocytes. The results indicate that MC administration drastically influences the pattern of expression of P-450 isoforms induced by PB in perivenular and midzonal hepatocytes.


1993 ◽  
Vol 11 (2) ◽  
pp. 231-239 ◽  
Author(s):  
F Aguado ◽  
J Rodrigo ◽  
L Cacicedo ◽  
B Mellström

ABSTRACT The distribution and regulation of mRNA for the IGF-I receptor (IGF-I-R) in the adult rat brain were studied by in-situ hybridization with a 35S-labelled cRNA probe. The pituitary gland showed a strong hybridization signal in the pars tuberalis (the surface of the median eminence), pars distalis and pars intermedia. Within the brain, a strong hybridization signal was found in the circumventricular organs, olfactory bulb, hippocampus, cerebellum and hypothalamus. IGF-I-R mRNA was consistently found in cell bodies of the hypothalamo-neurohypophysial systern. Six days of intermittent salt-loading resulted in an increase in IGF-I-R gene expression in the supraoptic nucleus. The increase in IGF-I-R mRNA was accompanied by a high expression of c-Fos immunoreactivity in the same cells. The presence and regulation of IGF-I-R mRNA in the hypothalamus suggest that IGF-I may affect the local plasticity or modulation of activated magnocellular neurones by an autocrine or paracrine action through specific receptors in the hypothalamo-neurohypophysial system.


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