METHODS OF ILEO-TRANSVERSE AND COLON INVAGINATIONAL ANASTOMOSES CREATING

Summary. The aim of the study. To improve the results of patients with tumors and non-neoplastic diseases of the colon treatment, to introduce into clinical practice the original surgical technique and methods of creating of colon anastomoses, which were tested in experiment. Materials and methods. Original methods of invaginational ileo-transverse and colon anastomoses creating were developed in the experiment on rabbits. Taking into account the obtained positive results, the methods of anastomoses formation were transferred to the surgical clinic and patents of Ukraine were obtained. In 2020, ileo-transverse and colonic anastomoses were formed in 134 patients on the basis of Transcarpathian Antitumor Center: one-row invaginational anastomosis according to the developed method in 22 patients (16.4 %), two-row manual — in 58 (43.3 %), circular stapler — in 36 (26.9 %), linear stapler anastomosis — in 4 (3 %), laparoscopic (linear stapler) — in 14 (10.4 %). Results and discussion. The most of complications occurred in the group with manual two-row anastomosis (16), in two cases the anastomotic leakage was recorded. When using a circular stapler suture, anastomotic leakage was observed in 1 patient, and anastomositis — in 4. When using linear stapler anastomoses, postoperative wound suppuration was observed in 1 patient. Conclusions. The most of early postoperative complications was observed after using a two-row manual colonic anastomosis (27.5 %). When using a circular stapler suture, the number of early postoperative complications was less than with a two-row manual anastomosis (22.2 % vs. 27.5 %, respectively). The least number of complications was recorded after the creation of a one-row invaginational anastomosis in the proposed original technique.

FORMATION OF THE COLON ANASTOMOSIS IN ORDER TO PROVIDE HIGH LEVEL OF MECHANICAL STRENGTH AND HERMETICITY

Summary. Aim of the study. Development and implementation of colon anastomoses formation method to improve their mechanical strength and biological hermeticity. Materials of the study. A comparative morphological study of the original and traditional two-row colonic anastomoses was performed in the experiment on 18 rabbits, and an assessment of their mechanical strength and biological hermeticity. The assessment of mechanical strength was defined by using a pneumopression technique. The assessment of biological hermeticity was defined by the method of culturing flushing water from the area of the anastomosis to the growth medium, species identification of isolated microorganisms using test systems and calculating the number of colony-forming units by quantitative method. Results of the research. The mechanical strength of one-row manual intra-nodular colonic anastomosis is higher by 66.3–85.4 %, respectively, of the first and seventh day of observations compared with the two-row manual anastomosis. During biological hermeticity assessment in the experimental group, in which colonic anastomosis was performed in the original method, the cultures were sterile at all stages of the sampling, whereas in the control group, where the traditional two-row manual anastomosis was formed, only from the seventh day all the cultures were sterile. Conslusions. The mechanical strength of one-row manual intra-nodular colonic anastomosis using atraumatic suture 4/0 without suturing the mucosa is higher by 66.3-85.4 %, respectively, of the first and seventh day of observations compared with the two-row manual anastomosis. Biological hermeticity of one-row manual intra-nodular colonic anastomosis is 100 % compared with the two-row manual anastomosis (28,5 %).

PS01.213: COMPARISON OF MANUAL VERSUS MECHANICAL INTRA-THORACIC ESOPHAGO-GASTRIC ANASTOMOSIS IN RADICAL 2-STAGE MINIMALLY INVASIVE ESOPHAGECTOMY FOR CANCER

Background While in open esophagectomy a plethora of studies comparing outcomes of mechanical (circular or linear stapler) versus hand-sewn intra-thoracic anastomosis have been published, little evidence exists regarding 2-stage minimally invasive esophagectomy; In the majority of published studies the mechanical anastomosis is favored. Construction of the intra-thoracic esophago-gastric anastomosis in minimally invasive esophagectomy is the procedure's rate limiting step. We aim to present our results of hand-sewn versus mechanical anastomosis in 2-stage minimally invasive esophagectomy. Methods Data of 113 consecutive patients over a 20-month period that underwent 2-stage minimally invasive esophagectomy for cancer in our institution were analyzed. Inclusion criteria included only 2-stage and only minimally invasive esophagectomies for cancer. 43 cases underwent fully minimally invasive esophagectomy and 70 had laparoscopic-assisted hybrid esophagectomy. A fully hand-sewn anastomosis with 3/0 v-lock barbed suture was formed in 38% of cases and a mechanical anastomosis with a 25mm or 28mm circular stapler was formed in 62% of cases. Comparison between anastomotic techniques was assessed through Chi-Square and Log-Rank analysis. Results Median age was 68(IQR,47–82) in manual anastomosis group and 65(IQR,31–81) in circular stapler group. Of the manual anastomosis group, 74% received neo-adjuvant treatment versus 20% of the mechanical anastomosis group. In the manual anastomosis group n = 3(6.97%) developed a leak; of these, n = 2 were stented and n = 1 was subclinical requiring no intervention. There was one sepsis-related death; 30-day mortality was 2.3%. In the mechanical anastomosis group n = 2(2,8%) developed anastomotic leak (one combined with tracheo-esophageal fistula) and both were stented and eventually resulted in mortality. 30-day mortality was 2.8%. No conduit necrosis was noticed. Anastomotic strictures requiring dilatation were seen in n = 4(9.3%) in the manual anastomosis group versus n = 5(7.1%) in the mechanical anastomosis group. No statistically significant difference was found between the groups in terms of leak (P = 0.312), stricture (P = 0.698) and mortality rate (P > 0.005). Median length of stay was 11 days (7–70) in the manual anastomosis group and 12 days (7–51) in mechanical anastomosis group. Conclusion Outcomes between manual and mechanical intra-thoracic anastomosis in minimally invasive esophagectomy show no difference within our study group. Both are equally safe and efficient, with surgeon's preference taking priority. Disclosure All authors have declared no conflicts of interest.

MECHANICAL SUTURE IN RECTAL CANCER

Background and aims. Colorectal cancer is one of the most frequent digestive malignancies, being the third cause of death by cancer, despite early diagnosis and therapeutic progress made over the past years. Standard treatment in these patients is to preserve the anal sphincter with restoration of intestinal function by mechanical colorectal anastomosis or coloanal anastomosis, and to maintain genitourinary function by preservation of hypogastric nerves.Methods. In order to emphasize the importance of this surgical technique in the Fourth Surgical Clinic of the CF Clinical Hospital Cluj-Napoca, we conducted a prospective observational interventional study over a 3-year period (2013-2016) in 165 patients hospitalized for rectal and rectosigmoid adenocarcinoma in various disease stages, who underwent Dixon surgery using the two techniques of manual and mechanical end-to-end anastomosis. For mechanical anastomosis, we used Covidien and Panther circular staplers. The patients were assigned to two groups, group A in which Dixon surgery with manual end-to-end anastomosis was performed (116 patients), and group B in which Dixon surgery with mechanical end-to-end anastomosis was carried out (49 patients).Results. Mechanical anastomosis allowed to restore intestinal continuity following low anterior resection in 21 patients with lower rectal adenocarcinoma compared to 2 patients in whom intestinal continuity was restored by manual anastomosis, with a statistically significant difference (p<0.000001). The double-row mechanical suture technique is associated with a reduced duration of surgery (121.67 minutes for Dixon surgery with mechanical anastomosis, compared to 165.931 minutes for Dixon surgery with manual anastomosis, p<0.0001).Conclusion. The use of circular transanal staplers facilitates end-to-end anastomosis by double-row mechanical suture, allowing to perform low anterior resection in situations when the restoration of intestinal continuity by manual anastomosis is technically not possible, with the aim to preserve the anal sphincter, to restore intestinal function and maintain genitourinary function through preservation of hypogastric nerves.

Comparative Analysis of Immediate Postoperative Complications Following Total Gastrectomy

Introduction: Total gastrectomy (TG), despite disrupting the continuity of the alimentary tract and accounting for significant postoperative complications, is the procedure of choice for curative resection of gastric carcinoma. The objectives of this study were to report the rate of postoperative complications following TG, to analyze adverse postoperative outcomes, and to determine the safer technique between Roux –en-Y Esophagojejunostomy and Omega Braun TG. Materials and Methods: A retrospective, observational study was conducted among patients diagnosed with gastric carcinoma who underwent TG between January 1st, 2010 and December 31st, 2012 in the Surgery Department of the Bucharest Clinical Emergency Hospital. Descriptive and analytical statistical analysis with parametric and non-parametric tests was carried out using GraphPad, with statistical significance set at p <0.05. Results: Seventy-seven patients, aged 37-91 years (average age 64.1 ± 11.59 years), were enrolled in this study. A total of 84 immediate postoperative complications were encountered in 35 patients (47.5%), classified into local (n=21, 25%) and general complications (n=63, 75%). Reoperation was necessary in five cases (6.2%), all after Roux-en-Y end-side esophagojejunostomy. Two deaths (n=2, 2.5%), one after Roux-en-Y and one after Omega-Braun TG, were reported. Discussion: The Roux-en-Y technique had the higher number of complications, both local and general. Omega-Braun TG was associated with a lower number of local complications; however, it was associated with life-threatening complications including hemodynamic instability and multisystem organ failure. A statistically significant correlation between manual anastomosis and mortality was observed. Conclusion: The study deemed Roux-en-Y Esophagojejunostomy the overall safer procedure. A statistically significant correlation between manual anostomosis and mortality was observed. Total Gastrectomy is a complex procedure with numerous potential complications which calls for an improved surgical technique to reduce postoperative risk.

High Grade Uterine and Rectal Prolapse

Introduction: Pelvic floor hernias are encountered especially in elderly women. A combined genital, bladder, and rectal prolapse poses treatment challenges in aged women. Case presentation: We present the case of an 88 year-old patient, complaining of an intravaginal mass protruding for the last 3 months, rectal prolapse that occurred two weeks before admittance, accompanied by stress incontinence of urine and chronic constipation. Examination revealed a uterine prolapse with cystocele and a fourth grade rectal prolapse. We decided on a perianal and transvaginal approach, performing preliminary dilatation and curettage, cervix amputation, anterior colporrhaphy and colpoperineorrhaphy (Manchester procedure) with perineal rectosigmoidectomy using the LigaSure™ device, and coloanal manual anastomosis. Postoperatively the patient had no symptoms of stress urinary incontinence, bowel movement resumed in the fourth postoperative day, and the patient was discharged after seven days. One month after surgery the patient has both urinary and fecal continence, with no relapse in pelvic organ prolapse. Conclusions: Encountering genital, bladder, and rectal prolapse in the same patient is quite rare, and its treatment can be difficult in aged women. Therefore, a less invasive surgical procedure, using the transvaginal approach, and a genital sparing surgery could be the key in cases like this.