Original record of deposited skins of hedgehogs (Erinaceidae) at red fox den

From 2015 to 2018, a total of 60 dens of red fox Vulpes vulpes have been visited in three sites of southern Tunisia. In this arid habitat, we recorded deposited skins of hedgehogs in front of 34 dens, an uncommon behaviour to our knowledge. These sightings suggest that red fox can be the main predator of hedgehogs in arid zones of North Africa.

Characterisation of seed dormancy of 12 Chilean species of Nolana (Solanaceae) from the coastal Atacama Desert

The genus Nolana (Solanaceae) comprises numerous species endemic to the coastal Atacama Desert of Chile and Peru of high ornamental potential and conservation value. The environments in which these species have evolved and are present today correspond to particular conditions in the midst of a hyper-arid habitat, so the study of their germination requirements and characterisation of seed dormancy becomes important in terms of conservation but also for ecological and evolutionary purposes. Different treatments were performed on mericarps of 12 species of Nolana: control (intact seeds imbibed in distilled water), scarification in funicular plug and distilled water and scarification in funicular plug and addition of GA3 (500 ppm); their permeability to water was also tested. It was determined that the species did not present physical dormancy, as had been previously reported, but rather physiological dormancy (PD). Germination results after treatments were not homogeneous among all 12 species, indicating differences in their dormancy levels. Also, the important role of the endosperm in the prevention of germination for the studied Nolana species was highlighted. Regarding the relationship between the level of PD (expressed as the percentage of germination for the most successful treatment) and the latitudinal distribution of the species or their phylogenetic closeness, it was determined that, for the studied species, their proximity in terms of clades was more relevant than their latitudinal distribution.

Estimating food resource availability in arid environments with Sentinel 2 satellite imagery

Background In arid environments, plant primary productivity is generally low and highly variable both spatially and temporally. Resources are not evenly distributed in space and time (e.g., soil nutrients, water), and depend on global (El Niño/ Southern Oscillation) and local climate parameters. The launch of the Sentinel2-satellite, part of the European Copernicus program, has led to the provision of freely available data with a high spatial resolution (10 m per pixel). Here, we aimed to test whether Sentinel2-imagery can be used to quantify the spatial variability of a minor tussock grass (Enneapogon spp.) in an Australian arid area and whether we can identify different vegetation cover (e.g., grass from shrubs) along different temporal scenarios. Although short-lasting, the Enneapogon grassland has been identified as a key primary food source to animals in the arid environment. If we are able to identify and monitor the productivity of this species remotely, it will provide an important new tool for examining food resource dynamics and subsequent animal responses to them in arid habitat. Methods We combined field vegetation surveys and Sentinel2-imagery to test if satellite spectral data can predict the spatial variability of Enneapogon over time, through GLMMs. Additionally, a cluster analysis (‘gower’ distance, ‘complete’ method), based on Enneapogon seed-productivity, and total vegetation cover in October 2016, identified three clusters: bare ground, grass dominated and shrub dominated. We compared the vegetation indices between these different clusters from October 2016 to January 2017. Results We found that MSAVI2 and NDVI correlated with the proportion of Enneapogon with seeds across the landscape and this relationship changed over time. Both vegetation indices (MSAVI2 and NDVI) were higher in patches with high seed-productivity of Enneapogon than in bare soil, but only in October, a climatically-favorable period during which this dominant grass reached peak seed-productivity. Discussion MSAVI2 and NDVI provided reliable estimates of the heterogeneity of vegetation type across the landscape only when measured in the Austral spring. This means that grass cover is related to seed-productivity and it is possible to remotely and reliably predict food resource availability in arid habitat, but only in certain conditions. The lack of significant differences between clusters in the summer was likely driven by the short-lasting nature of the vegetation in the study and the sparseness of the grass-dominated vegetation, in contrast to the shrub vegetation cluster that was particularly well measured by the NDVI. Conclusions Overall, our study highlights the potential for Sentinel2-imagery to estimate and monitor the change in grass seed availability remotely in arid environments. However, heterogeneity in grassland cover is not as reliably measured as other types of vegetation and may only be well detected during periods of peak productivity (e.g., October 2016).

Crowding, group size and population structure of the Blackbuck Antilope cervicapra (Linnaeus, 1758) (Mammalia: Cetartiodactyla: Bovidae) in the semi-arid habitat of Haryana, India

To understand the population growth dynamics and life history parameters of the Blackbuck, 24 fortnightly visits were made in and around Lalpur Jheel, Dobhi Village of district Hisar (Haryana) from March 2017 to February 2018. Scan sampling method was used to record the Blackbuck population. In the present study, a total of 68 sightings of Blackbuck were made including a minimum of one sighting per visit to a maximum of seven sightings per visit with group size varying from one individual to a maximum of 58 individuals per sighting. The overall mean group size and crowding of the Blackbuck population were 13.84 ± 1.89 S.E. and 31.31 (N=941 individuals), respectively. The population structure of Blackbuck revealed six different age and sex classes, namely, adult male, adult female, sub-adult male, sub-adult female, yearling male, and fawn. As far as the social organization of the Blackbuck is concerned, six different types of social grouping were recorded, namely lone territorial male (adult male), unimale-unifemale (adult male and adult female), bachelor herd (adult male(s)/ sub-adult male(s)/ yearling male(s)), mixed herd (adult male(s)/ sub-adult male(s)/ yearling male(s)/ adult female(s)/ sub-adult female(s)/ fawn(s)), harem herd (1 adult male/ adult female(s)/ sub-adult female(s)/ fawn(s)), and female herd (adult female(s)/ sub-adult female(s)/ fawn(s)). It was concluded that Blackbuck shows partial social organization as both the solitary and herd were observed during the present study.

Phylogenomic history of enigmatic pygmy perches: implications for biogeography, taxonomy and conservation

Pygmy perches (Percichthyidae) are a group of poorly dispersing freshwater fishes that have a puzzling biogeographic disjunction across southern Australia. Current understanding of pygmy perch phylogenetic relationships suggests past east–west migrations across a vast expanse of now arid habitat in central southern Australia, a region lacking contemporary rivers. Pygmy perches also represent a threatened group with confusing taxonomy and potentially cryptic species diversity. Here, we present the first study of the evolutionary history of pygmy perches based on genome-wide information. Data from 13 991 ddRAD loci and a concatenated sequence of 1 075 734 bp were generated for all currently described and potentially cryptic species. Phylogenetic relationships, biogeographic history and cryptic diversification were inferred using a framework that combines phylogenomics, species delimitation and estimation of divergence times. The genome-wide phylogeny clarified the biogeographic history of pygmy perches, demonstrating multiple east–west events of divergence within the group across the Australian continent. These results also resolved discordance between nuclear and mitochondrial data from a previous study. In addition, we propose three cryptic species within a southwestern species complex. The finding of potentially new species demonstrates that pygmy perches may be even more susceptible to ecological and demographic threats than previously thought. Our results have substantial implications for improving conservation legislation of pygmy perch lineages, especially in southwestern Western Australia.