Preferential Begging Responses of Shiny Cowbirds to the Conspecific Chatter Call

Avian brood parasites lay their eggs into the nests of other species, which incubate them and raise the chicks until their independence. Despite living their early weeks of life surrounded by heterospecifics, young brood parasites have the ability to recognize and associate to conspecifics after independence. It has been proposed that the initial conspecific recognition develops when a young parasite encounters a unique species-specific signal that triggers the learning of other aspects of the producer of the signal. For cowbirds (Molothrus spp.), this species-specific signal is hypothesized to be the chatter call. Young birds also could express auditory biases, which in some cases lead to discrimination in favor of conspecific songs. Therefore, the perceptual selectivity for chatters might be also present in nestlings. Our aim was to assess if nestlings of the shiny cowbird (M. bonariensis) present a preferential begging response to conspecific chatter calls. We evaluated if they respond more to the parasitic vocalization than host chicks and if they respond more to the chatter than to heterospecific nonhost calls. We tested shiny cowbird chicks reared by chalk-browed mockingbirds (Mimus saturninus) or house wrens (Troglodytes aedon) and host chicks, as control species. We randomly presented to 6-day-old chicks the following playback treatments: (1) conspecific chatter calls, (2) host calls, used as positive controls, and (3) nonhost calls, used as negative control. We measured if chicks begged during the playback treatments and the begging intensity. When responding to the playback of chatter calls, shiny cowbird chicks begged at a higher frequency and more intensively than host chicks. Shiny cowbird chicks reared by mockingbirds begged more intensively to playbacks of conspecific chatter calls than to host calls, while those reared by wrens begged with a similar intensity to playbacks of conspecific chatter and host calls. On the contrary, wren nestlings begged more intensively to playbacks of the wren call than to chatter calls. Mockingbird nestlings did not beg during any treatment. None of the three species begged during the playback of nonhost calls. Our results show that the chatter call produced a preferential begging response in cowbird nestlings, which may be the beginning of a process of conspecific recognition.

Agonistic Behavior Is Affected by Memory in the Dusky Damselfish Stegastes fuscus

The ability to discriminate familiar from unfamiliar conspecifics has been demonstrated in several species of fish. Agonistic interactions are among the most frequent behaviors exhibited by territorial species and could offer useful information for the individual recognition process. In agonistic situations, memory may modulate the behavioral response and affect social dynamics, but few studies have explored the memory retention acquired during aggressive encounters. The present study investigated the memory retention of an agonistic encounter in the dusky damselfish Stegastes fuscus. The experimental procedure was divided into three parts: (1) Familiarization; (2) Recognition test; and (3) Memory test. During the familiarization phase, the fish were visually exposed to the same conspecific for 5 days (10 min per day) and the behavior was recorded. On the following day (conspecific recognition test), half of the animals were paired with the same conspecific and the other half with a different conspecific for 10 min, and the behavior was recorded. The fish were retested 5, 10, and 15 days after the test to evaluate memory retention. In the memory test, they were exposed to the same conspecific as before or to a different conspecific. We found that the damselfish reduced their agonistic displays when the stimulus fish was familiar, but when it was unfamiliar, the animals were more aggressive and only reduced their mnemonic response after 10 days. These results suggest that the recognition ability of damselfish can be affected by time and that it modulates agonistic response.

Behavioral traits and territoriality in the symbiotic scaleworm Ophthalmonoe pettiboneae

Among marine invertebrates, polychaete worms form symbiotic associations showing a wide variety of host use patterns. Most commonly, they live solitary on hosts, likely resulting from territorial behavior, yet little is known of the precise nature of the involved interactions. Based on field and laboratory observations, we described the symbiotic association between Ophthalmonoe pettibonae and Chaetopterus cf. appendiculatus from Nhatrang Bay (Vietnam). Then, by experimentally manipulating the competitor-to-resource ratio, we analyzed symbiont behavior and we assessed whether the 1:1 uniform distribution observed in nature could be driven by agonistic territorial behavior. Hosts and symbiont populations had low densities, lacked size relationships and showed higher prevalence when denser. Symbiont behavior included territoriality, expressed through conspecific recognition and intraspecific aggressive interactions (pursuit and escaping, hiding, choosing position, aggressive fighting, and targeting a specific bite zone). Our experiments proved that territoriality led to host monopolization by a single symbiont, provided the first empirical evidence that symbiont body injuries were caused during territorial contests, and allowed us to first suggest that a marine symbiotic invertebrate may control a territory extending beyond its host, even including neighboring hosts. Overall, this is the first report of such a complex symbiotic behavior for an annelid polychaete.

Conspecific recognition of pedal scent in domestic dogs

Carnivores rely heavily on scent to communicate with conspecifics. Scent glands located on the underside of the feet provide an especially efficient way of leaving a scent trail. Although domestic dogs (Canis familiaris) are well-known for their olfactory abilities and scent marking behaviours, their use of pedal scent for communication remains unknown. We studied the reaction of intact dogs of both sexes to male and female pedal scent as well as a control sample of scent taken from the ground, using sniffing time and nostril usage as an indicator of interest level and emotional valence. In male subjects, only the sniffing duration for other males differed from the control samples, with no clear difference detected between male and female scent. Females showed no difference in the sniffing duration for any sample type. Conversely, male nostril use did not differ between the sample types, whereas females demonstrated a right nostril bias when sniffing the scent from other females and a left nostril bias when sniffing the control. We have shown that dogs recognize scent taken from the pedal glands from other dogs, although the extent to which they use this information to determine the sex of the scent depositor remains unclear.

Expressed Vomeronasal Type-1 Receptors (V1rs) in Bats Uncover Conserved Sequences Underlying Social Chemical Signaling

In mammals, social and reproductive behaviors are mediated by chemical cues encoded by hyperdiverse families of receptors expressed in the vomeronasal organ. Between species, the number of intact receptors can vary by orders of magnitude. However, the evolutionary processes behind variation in receptor number, and its link to fitness-related behaviors are not well understood. From vomeronasal transcriptomes, we discovered the first evidence of intact vomeronasal type-1 receptor (V1r) genes in bats, and we tested whether putatively functional bat receptors were orthologous to those of related taxa, or whether bats have evolved novel receptors. Instead of lineage-specific duplications, we found that bat V1rs show high levels of orthology to those of their relatives, and receptors are under comparative levels of purifying selection as non-bats. Despite widespread vomeronasal organ loss in bats, V1r copies have been retained for >65 million years. The highly conserved nature of bat V1rs challenges our current understanding of mammalian V1r function and suggests roles other than conspecific recognition or mating initiation in social behavior.

Real or fake? Natural and artificial social stimuli elicit divergent behavioural and neural responses in mangrove rivulus, Kryptolebias marmoratus

Understanding how the brain processes social information and generates adaptive behavioural responses is a major goal in neuroscience. We examined behaviour and neural activity patterns in socially relevant brain nuclei of hermaphroditic mangrove rivulus fish ( Kryptolebias marmoratus ) provided with different types of social stimuli: stationary model opponent, regular mirror, non-reversing mirror and live opponent. We found that: (i) individuals faced with a regular mirror were less willing to interact with, delivered fewer attacks towards and switched their orientation relative to the opponent more frequently than fish exposed to a non-reversing mirror image or live opponent; (ii) fighting with a regular mirror image caused higher expression of immediate-early genes (IEGs: egr-1 and c-Fos ) in the teleost homologues of the basolateral amygdala and hippocampus, but lower IEG expression in the preoptic area, than fighting with a non-reversing mirror image or live opponent; (iii) stationary models elicited the least behavioural and IEG responses among the four stimuli; and (iv) the non-reversing mirror image and live opponent drove similar behavioural and neurobiological responses. These results suggest that the various stimuli provide different types of information related to conspecific recognition in the context of aggressive contests, which ultimately drive different neurobiological responses.