diol dehydratase
Recently Published Documents


TOTAL DOCUMENTS

106
(FIVE YEARS 2)

H-INDEX

26
(FIVE YEARS 0)

Author(s):  
Borislav Kovačević ◽  
Luka Bilić ◽  
Danijela Barić ◽  
Gregory M Sandala ◽  
David M Smith

Author(s):  
Alejandro Ramirez Garcia ◽  
Jianbo Zhang ◽  
Anna Greppi ◽  
Florentin Constancias ◽  
Esther Wortmann ◽  
...  

2020 ◽  
Vol 202 (9) ◽  
Author(s):  
Alex P. Lundin ◽  
Katie L. Stewart ◽  
Andrew M. Stewart ◽  
Taylor I. Herring ◽  
Chiranjit Chowdhury ◽  
...  

ABSTRACT Bacterial microcompartments (MCPs) are widespread protein-based organelles composed of metabolic enzymes encapsulated within a protein shell. The function of MCPs is to optimize metabolic pathways by confining toxic and/or volatile pathway intermediates. A major class of MCPs known as glycyl radical MCPs has only been partially characterized. Here, we show that uropathogenic Escherichia coli CFT073 uses a glycyl radical MCP for 1,2-propanediol (1,2-PD) fermentation. Bioinformatic analyses identified a large gene cluster (named grp for glycyl radical propanediol) that encodes homologs of a glycyl radical diol dehydratase, other 1,2-PD catabolic enzymes, and MCP shell proteins. Growth studies showed that E. coli CFT073 grows on 1,2-PD under anaerobic conditions but not under aerobic conditions. All 19 grp genes were individually deleted, and 8/19 were required for 1,2-PD fermentation. Electron microscopy and genetic studies showed that a bacterial MCP is involved. Bioinformatics combined with genetic analyses support a proposed pathway of 1,2-PD degradation and suggest that enzymatic cofactors are recycled internally within the Grp MCP. A two-component system (grpP and grpQ) is shown to mediate induction of the grp locus by 1,2-PD. Tests of the E. coli Reference (ECOR) collection indicate that >10% of E. coli strains ferment 1,2-PD using a glycyl radical MCP. In contrast to other MCP systems, individual deletions of MCP shell genes (grpE, grpH, and grpI) eliminated 1,2-PD catabolism, suggesting significant functional differences with known MCPs. Overall, the studies presented here are the first comprehensive genetic analysis of a Grp-type MCP. IMPORTANCE Bacterial MCPs have a number of potential biotechnology applications and have been linked to bacterial pathogenesis, cancer, and heart disease. Glycyl radical MCPs are a large but understudied class of bacterial MCPs. Here, we show that uropathogenic E. coli CFT073 uses a glycyl radical MCP for 1,2-PD fermentation, and we conduct a comprehensive genetic analysis of the genes involved. Studies suggest significant functional differences between the glycyl radical MCP of E. coli CFT073 and better-studied MCPs. They also provide a foundation for building a deeper general understanding of glycyl radical MCPs in an organism where sophisticated genetic methods are available.


2019 ◽  
Vol 123 (29) ◽  
pp. 6178-6187 ◽  
Author(s):  
Luka Bilić ◽  
Danijela Barić ◽  
Radha Dilip Banhatti ◽  
David M. Smith ◽  
Borislav Kovačević

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Jianbo Zhang ◽  
Christophe Lacroix ◽  
Esther Wortmann ◽  
Hans-Joachim Ruscheweyh ◽  
Shinichi Sunagawa ◽  
...  

2017 ◽  
Vol 40 ◽  
pp. 148-156 ◽  
Author(s):  
Jia Wang ◽  
Rachit Jain ◽  
Xiaolin Shen ◽  
Xinxiao Sun ◽  
Mengyin Cheng ◽  
...  

2017 ◽  
Vol 70 (4) ◽  
pp. 367 ◽  
Author(s):  
Ganna Gryn'ova ◽  
Michelle L. Coote

Accurate quantum-chemical calculations are used to analyze the effects of charges on the kinetics and thermodynamics of radical reactions, with specific attention given to the origin and directionality of the effects. Conventionally, large effects of the charges are expected to occur in systems with pronounced charge-separated resonance contributors. The nature (stabilization or destabilization) and magnitude of these effects thus depend on the orientation of the interacting multipoles. However, we show that a significant component of the stabilizing effects of the external electric field is largely independent of the orientation of external electric field (e.g. a charged functional group, a point charge, or an electrode) and occurs even in the absence of any pre-existing charge separation. This effect arises from polarization of the electron density of the molecule induced by the electric field. This polarization effect is greater for highly delocalized species such as resonance-stabilized radicals and transition states of radical reactions. We show that this effect on the stability of such species is preserved in chemical reaction energies, leading to lower bond-dissociation energies and barrier heights. Finally, our simplified modelling of the diol dehydratase-catalyzed 1,2-hydroxyl shift indicates that such stabilizing polarization is likely to contribute to the catalytic activity of enzymes.


2016 ◽  
Vol 89 (8) ◽  
pp. 955-964 ◽  
Author(s):  
Kazuki Doitomi ◽  
Takashi Kamachi ◽  
Tetsuo Toraya ◽  
Kazunari Yoshizawa

Biochemistry ◽  
2015 ◽  
Vol 55 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Tetsuo Toraya ◽  
Aya Tanokuchi ◽  
Ai Yamasaki ◽  
Takehiro Nakamura ◽  
Kenichi Ogura ◽  
...  
Keyword(s):  

2014 ◽  
Vol 87 (9) ◽  
pp. 950-959 ◽  
Author(s):  
Kazuki Doitomi ◽  
Hiromasa Tanaka ◽  
Takashi Kamachi ◽  
Tetsuo Toraya ◽  
Kazunari Yoshizawa

Sign in / Sign up

Export Citation Format

Share Document