Analysis of the use of media resulting from bioconversion of organic waste in the production of maggots BSF (black soldier fly)

Maggots is an organism derived from the eggs of the black fly, Hermentia illucens (black soldier fly, BSF), which undergoes metamorphosis in the second phase after the egg phase and before the pupa phase which then turns into an adult fly. The purpose of this study was to analyze the utilization of organic waste substrate on the production of BSF maggots cultivation. This research was conducted outdoor at the Freshwater Aquaculture Fisheries Center (BPBAT) Sungai Gelam Jambi with a completely randomized design (CRD) with 4 treatments and 3 replications; Treatment A: PKM (palm kernel meal) 100%, B (PKM 50% + cabbage vegetable waste 50%), C (PKM 50% + coconut pulp 50%) and D (PKM 50% + coconut pulp 25% + vegetable waste cabbage 25%). The average yield parameter of high maggots biomass in treatment A was 673.67 g/4 kg substrate and the lowest biomass in treatment D was 239.67 g/4 kg substrate. For the average weight and length of the best maggots in treatment A (0.20 g/individual) and (1.83 cm), the lowest was in B (0.12 g/Ind. and 1.58 cm). The highest was in treatment B (5,182.31 individual/4 kg substrate) and the lowest was in D (1,479.44 ind./4 kg substrate. The highest bioconversion value of maggots to organic matter OSE (organic substrate efficiency) was highest in treatment A (16, 84%) and the lowest was in D (5.99%). Technically, treatment A was slightly better than B, while economically the best organic substrate medium for maggots cultivation was in treatment B with a production cost of Rp. 7.257 and the ECR (economic conversion ratio) value of 5.81 was lower than the other 3 treatments. Keywords: Maggots, black soldier fly, Hermentia illucens, organic waste. ABSTRAK Maggots merupakan organisme yang berasal dari telur seranga lalat hitam, Hermentia illucens (black soldier fly, BSF). Tujuan penelitian ini yaitu menganalisis pemanfaatan subtrat limbah organik terhadap produksi budidaya maggots BSF. Penelitian ini dilaksanakan di Balai Perikanan Budidaya Air Tawar (BPBAT) Sungai Gelam, Jambi dengan rancangan acak lengkap (RAL) 4 perlakuan 3 ulangan yaitu perlakuan A : PKM (palm kernel meal) 100%, B (PKM 50% + limbah sayur kol 50%), C (PKM 50% + ampas kelapa 50%), dan D (PKM 50% + ampas kelapa 25%+ limbah sayur kol 25%).Rata-rata biomassa tertinggi didapatkan pada perlakuanA (673,67g/4 kg subtrat) dan biomassa terendah dihasilkan pada perlakuan D (239.67g/4 kg subtrat). Untuk bobot rata-rata dan panjang maggots terbaik dihasilkan pada perlakuan A (0,20 g/individu dan 1,83 cm/individu), terendah pada B (0,12 g/individu dan (1,58 cm). Jumlah populasi maggots yang terbanyak dihasilkan pada perlakuan B (5.182,31 ind./4 kg subtrat) dan terendah pada D (1.479,44 individu/4 kg subtrat). Nilai biokonversi maggots terhadap bahan organik OSEterbaik (organic substrate efficiency) tertinggi pada perlakuan A (16,84%) dan terendah pada D (5,99%). Secara teknis perlakuan A sedikit lebih baik dari B sedangkan secara ekonomi media subtrat organik terbaik untuk budidaya maggots terdapat pada perlakuan B dengan biaya produksi sebesar Rp. 7.257 dan nilai ECR (economic convertion ratio) sebesar 5.81 lebih rendah dari ke 3 perlakuan lainnya. Kata kunci: Maggots, black soldier fly, Hermentia illucens, limbah organik.

Replacing Fishmeal With Palm Kernel Meal In Formulated Feed For The Pacific White Shrimp (Litopenaeus Vannamei)

Utilisation of plant proteins to replace fish meal in shrimp feeds has become an important consideration because fish meal is becoming more expensive due to increasing demand worldwide. The potential use of palm kernel meal (PKM) in this study to substitute fish meal in the Pacific white shrimp (Litopenaeus vannamei) diets was evaluated by conducting a 90-day feeding trial. Shrimp juveniles with an initial average weight of 0.5 g, protein content of 10.74±0.70% were randomly distributed into five treatments in triplicates. Four isonitrogenous (approximately 35% protein) diets were formulated to contain 0% (D0), 25% (D25), 50% (D50) and 75% (D75) of PKM replacement and a commercial feed served as control treatment (Control). Results from this study revealed that shrimps fed D25 were comparable with those fed with Control as there was no significant difference (p>0.05) in weight gain and specific growth rate (SGR) between the groups. However, PKM inclusions above 50% showed detrimental effects on the growth performance. The highest total protein percent was observed in shrimp tissues fed with D25 (67.59±0.87%) and D75 showed the lowest protein among the treatments (57.4±0.63%) (p<0.05). Total lipid content was observed high in shrimps fed with Control (4.33±2.96%) and decreased with PKM replacement levels. The highest carbohydrate was found in shrimps fed with D75 (16.75±0.04%) and the lowest was found in shrimps fed with D25 (14.67±0.07%). When PKM is utilised to replace FM, a limit of 25% level should be recommended.

Effect of dietary palm kernel meal on laying hens

Indonesia has the potential for palm kernel meal, which has the largest production in the world. Palm kernel meal has been commonly used as an ingredient in ruminant and poultry feeds. However, the various studies conducted have produced very varied results. This article aimed to analyze various research data on the use of palm kernel meal in the chicken feed. The data used were 38 data points from 10 journal articles. The use of palm kernel meal ranged from 0% to 70% in rations. The statistical model used is linear regression. The database was analyzed using SPSS software version 25. Results showed that the use of palm kernel meal in laying hens reduced (P<0.01) egg production. Palm kernel meal also increased FCR (P<0.05) and tended to decrease Haugh Unit (P<0.1). Other parameters such as consumption, egg weight, mortality, egg shell thickness, and yolk color were not influenced by dietary inclusion of palm kernel meal. In conclusion, palm kernel meal cannot be given to poultry directly, it is necessary to process the ingredient to enhance its nutritive value.

Estimation rumen degradable protein of local feeds in dairy cattle using in sacco method

There is a lack of nutrition information on local feeds protein in Indonesia, especially to determine protein fraction of dairy feed. The objective of this study was to determine rumen degradable protein (RDP) of local feeds in dairy cattle using in sacco method. The local feeds are copra meal, palm kernel meal, coffee husk, tofu waste, soy-sauce waste, brewer waste, and habbatussauda waste. Two ruminal fistulated male Frisian Holstein were used to determine rumen degradable protein using in sacco method. The parameters observed include the estimated kinetic parameters, effective degradability (ED), and RDP. The parameters were evaluated with analysis of variance using SAS University software. The result showed that tofu waste and habbatussauda waste had high potential rapid degradation of CP following by brewer waste, palm kernel waste, soy-sauce waste, copra meal, and coffee husk. Habbatussauda waste had higher RDP followed by brewer waste, tofu waste, copra meal, palm kernel meal, soy-sauce waste, and coffee husk. There was a positive correlation between RDP and crude protein content, and a negative correlation between RDP and crude fiber. It is concluded that local feeds have various characteristics of quality feed protein, which is shown by rumen degradable protein and rumen undegradable protein.

Optimal Replacement of Soybean Meal with Fermented Palm Kernel Meal as Protein Source in a Fish Meal-Soybean Meal-Based Diet of Sex Reversed Red Tilapia (Oreochromis niloticus × O. mossambicus)

The solid-state fermentation by effective microorganisms (containing photosynthetic bacteria, lactic acid bacteria, nitrogen-fixing bacteria, yeast and Bacillus sp.) improved the nutritive values of palm kernel meal (PKM). Increased crude protein (20.79%), nitrogen-free extract (40.07%) and gross energy (19.58%) were observed in fermented PKM (FPKM) relative to raw PKM while crude lipid (15.65%), crude fiber (36.45%) and ash (29.54%) were decreased. Replacement of soybean meal (SBM) with FPKM as a protein source was investigated for its effects in sex-reversed red tilapia (Oreochromis niloticus × O. mossambicus). The two-month-old fish (14.85 ± 0.28 g initial weight) were fed fish meal-SBM-based diets with replacement by FPKM at 25% (25FPKM), 50% (50FPKM), 75% (75FPKM) and 100% (100FPKM), while an FPKM-free diet (0FPKM) was used as a control. The five treatments, comprising triplicate cement ponds and forty fish each, were conducted in a recirculating system over 12 weeks. At the end of the feeding trial, fish fed the 50FPKM diet were superior in growth performance, while the feed utilization parameters were similar across all five treatments. Physiological adaptation of the protein-digesting (pepsin and trypsin) and lipid-digesting (lipase) enzymes was detected at all protein replacement levels (except for 25FPKM), as well as of the enzyme for cellulose digestion (cellulase), but not of the carbohydrate-digesting enzymes (amylase). Protein synthesis capacity in flesh was improved in fish fed the 50FPKM diet, while the quality of the main flesh proteins, actin and myosin, showed no significant differences across the five treatments. No differences in carcass composition and no negative effects on hematological parameters or liver histoarchitecture at the 50% replacement level of SBM by FPKM also support this alternative. Findings from the current study indicate the low-cost FPKM-containing diet for tilapia in comparison with control diet.

Cellulolytic and mannanolytic aerobic bacteria isolated from Buffalo rumen (Bubalus babalis) and its potency to degrade fiber in palm kernel meal

Sari SLA, Triyanto T, Zuprifal Z, Prijambada ID. 2021. Cellulolytic and mannanolytic aerobic bacteria isolated from Buffalo rumen (Bubalus babalis) and its potency to degrade fiber in palm kernel meal. Biodiversitas 22: 2829-2837. Palm kernel meal (PKM) is potential to be used as feed, but its high fiber content causes PKM meal difficult to be digested by monogastric animals. Ruminants are especially effective in digesting plant fibers because of the presence of microbes in their rumens. Based on those facts, this research was conducted to obtain mannanolytic and cellulolytic bacteria from buffalo rumens (Bubalus babalis, Linnaeus, 1758), which can degrade fibers in PKM. Bacteria were isolated from buffalo rumen by using PKM- isolation media. Screening of hydrolytic activities was done based on clear zone formation on screening media. A total of five bacterial isolates with the highest hydrolytic activities were then assayed quantitatively for their abilities to degrade mannan and cellulose, then identified based on 16S rRNA gene sequences. This research successfully isolated 34 bacterial isolates. The screening result demonstrated that all isolates could hydrolyze mannan, cellulose and polysaccharide in PKM. Isolate BR25 showed the highest hydrolytic ability on PKM and mannan screening media with clear zone diameter/colony diameter ratio (dz/dc ratio) of 2.99 and 3.53, respectively. Isolate BR31 showed the highest cellulolytic ability with dz/dc ratio value of 2.22. Five isolates with the highest hydrolytic activity, i.e. BR14, BR16, BR23, BR25, and BR30 showed the ability to grow on submerged media which contain locust bean gum (LBG) and microcrystalline cellulose (MCC) respectively, as single carbon source and isolate BR25 showed the highest ability to degrade mannan and cellulose. Based on the gene sequence of 16S rRNA, isolates BR14, BR16, BR23, BR25, and BR30 were identified to be closely related to Exiguobacterium acetylicum, Bacillus cereus, Klebsiella quasipneumoniae, Paenibacillus polymyxa, and Acinetobacter baumannii with 98.57-100% level of similarity.