Micropropagation of Pogostemon cablin Benth. through Direct Regeneration for Production of True to Type Plants

Multiple shoots from nodal explants of Pogostemon cablin Benth. MS supple-mented with 0.5 mg/l BAP initiated a mean of 45.66 shoots/nodal explant. Within four weeks of initiation, regenerated multiple shoots attained a height of 3.6 cm. Subsequent transfer of these in vitro derived nodal segments onto MS supplemented with BAP and Kn ( 0.5 mg/l), induced a mean of 62.45 shoots. Higher concentration of either BAP or Kn more than 0.5 mg/l resulted in callus proliferation and showed hyperhydric shoots with morphological abnormalities. Rooting was readily achieved upon transfer of shoots on half strength MS supplemented with 100 mg/l activated charcoal. Rooted shoots, following acclimatization in greenhouse, were successfully transferred to soil with 91% survival. Also shoots regenerated in vitro were directly transplanted to soil and acclimatized. Tissue cultured plants were analyzed for oil content by employing gas chromatography and found that the patterns were similar to mother plants. This confirms the true to type nature of micropropagated plants. Key words: Micropropagation, Direct regeneration, Pogostemon cablin, organogenesis D.O.I. 10.3329/ptcb.v20i1.5971 Plant Tissue Cult. & Biotech. 20(1): 81-89, 2010 (June)

In vitro Propagation of Mentha viridis L. from Nodal and Shoot tip Explants

A simple micropropagation method from nodal and shoot tip explants is reported here for Mentha viridis, an economically important medicinal plant. High frequency of microshoots was obtained from these two explants on MS supplemented with various concentrations of BAP (1.0 - 4.0 mg/l) and Kn (1.0 -4.0 mg/l). Maximum number of shoots was obtained from nodal explants in the medium containing 3.0 mg/l BAP. The root induction was carried out by using IBA and IAA (0.5 - 2.0 mg/l). Among these, high frequency of root proliferation was achieved in the medium containing 1.5 mg/l of IBA. The rooted plantlets were hardened and transferred to the field. The survival rate was 90 - 95% after 25 days. Key words: Micropropagation, Mentha viridis, Node, Shoot tip, Multiple shoots D.O.I. 10.3329/ptcb.v18i1.3243 Plant Tissue Cult. & Biotech. 18(1): 1-6, 2008 (June)

Micropropogation of Cucurbita maxima Duch. through Shoot Tip Culture

Micropropagation of pumpkin (Cucurbita maxima Duch.) was achieved using shoot tip of in vitro grown seed derived plants of two cultivars namely, Bikrompuri and Baromasi of Bangladesh. The excised shoot tips were cultured on MS medium containing KIN, BA, NAA at various levels of concentration and combination for shoot induction and proliferation, and best response was found at 3.0 mg/l of BA. Shoots were rooted most effectively in ½ MS medium supplemented with 1.0 mg/l IBA. Bikrompuri was found more responsive than Baromasi for rapid clonal propagation. Key words: Micropropagation, Direct organogenesis, Shoot tip, Pumpkin DOI:10.3329/jbs.v16i0.3742 J. bio-sci. 16: 59-65, 2008

High Frequency In Vitro Propagation of Adhatoda vasica Nees through Shoot Tip and Nodal Explants Culture

An efficient protocol for in vitro propagation of Adhatoda vasica Nees was established using shoot tip and nodal explants from field grown mature plant. Proliferation of multiple shoots was achieved on MS medium supplemented with different concentrations and combinations of cytokinins (0.5-4.0 mg/l) and auxins (0.1-1.0 mg/l). Maximum number of shoots per explant (13.0) was obtained on MS medium supplemented with 2.0 mg/l BAP + 0.2 mg/l NAA. Among two types of explants used in this study, nodal explants showed better response in respect of multiple shoot production. The elongated shoots were excised and subcultured for rooting on MS medium supplemented with different concentrations of auxins (IBA and NAA). Highest 80% rooting was achieved; and three to four roots per shoot were recorded in medium with 1.0 mg/l IBA within 4 weeks of culture. The in vitro raised plantlets were acclimatized and successfully transferred to natural condition in pot. The regenerated plants were healthy, uniform and identical to the donor plants and the survival percentage was 80%. Key words: Micropropagation, Adhatoda vasica, shoot tip, nodal explant. DOI:10.3329/jbs.v16i0.3739 J. bio-sci. 16: 35-39, 2008