Clinical evaluation of microRNA-145 expression in renal cell carcinoma: a promising molecular marker for discriminating and staging the clear cell histological subtype

The vast majority of malignancies detected in renal parenchyma are diagnosed as renal cell carcinoma (RCC), whose subtype discrimination and determination of prognosis may contribute to the selection of the adequate therapy. Recently, a new class of small non-coding RNAs, known as microRNAs, has proven to be among the most promising biomarkers for providing this information. Herein, we sought to add up to this knowledge by evaluating the expression levels of microRNA-145 (miR-145) in RCC. For that purpose, total RNA from 58 cancerous and 44 adjacent non-cancerous renal tissues was firstly extracted and then polyadenylated and reverse transcribed to cDNA. MiR-145 levels were finally analyzed by developing and applying a highly sensitive real-time PCR protocol, while their clinical significance was determined via comprehensive statistical analysis. Our data showed that miR-145 was significantly downregulated in cancerous samples and could discriminate between clear cell and non-clear cell subtypes. Moreover, miR-145 expression was found to be correlated with primary tumor staging of cancerous samples, something also noticed in the clear cell RCC subset, in which miR-145 levels were negatively correlated with tumor size as well. Overall, these results indicate that miR-145 might constitute a promising molecular marker for RCC classification and staging.

GAMBARAN ULTRASONOGRAFI HEPAR DI BAGIAN RADIOLOGI FK UNSRAT BLU RSUP PROF.DR. R. D. KANDOU MANADO PERIODE MARET – JUNI 2014

Ultrasonography of the liver is an accurate imaging modality for focal or diffuse liver disease, determine the primary tumor staging, detecting secondary deposits, investigation of calculus and jaundice, and as an aid in liver biopsy or interventional procedures. The purpose of this study was to describe the results of liver ultrasonography in the Department of Radiology of Prof. Dr RD Kandou Hospital Manado from March 1 to June 30, 2014. This study is a retrospective descriptive study by using secondary data from medical records contained in the department of radiology BLU Prof. Dr. R. D. Kandou Manado from March to June 2014. Overall hepatic ultrasonography results found 77 picture, with a picture of the liver more in women (19.5 %). Conclusions: Abnormal liver ultrasound picture of the highest in the age group 36-45 years, 46-55 years, 56-65 years (23.4 %). Most liver ultrasound appearance is an appearance of fatty liver ultrasonography (37.7 %). We recommend that patients come with complaints such as chronic abdominal pain, and repeatedly confirmed the cause through the abdominal ultrasound examination, to help diagnose, exclude other abdominal disorders and prevent displacement cause abdominal pain.Keywords: liver ultrasonography, liver diseaseAbstrak: Ultrasonografi hepar merupakan modalitas pencitraan yang akurat untuk penyakit hati fokal atau difus, menentukan staging tumor primer, mendeteksi deposit sekunder, pemeriksaan penunjang untuk kalkulus dan jaundice, dan sebagai bantuan pada biopsi hati atau prosedur intervensional. Tujuan Penelitian ini untuk mengetahui gambaran hasil Ultrasonografi hepar di Bagian Radiologi FK UNSRAT/SMF Radiologi BLU RSUP Prof. Dr. R. D. Kandou Manado Periode 1 Maret – 30 Juni 2014. Penelitian ini merupakan penelitian deskriptif retrospektif dengan memanfaatkan data sekunder berupa catatan medik yang terdapat di Bagian Radiologi BLU RSUP Prof. Dr. R. D. Kandou Manado Periode Maret – Juni 2014. Keseluruhan hasil Ultrasonografi hepar ditemukan 77 gambaran, dengan gambaran hepar lebih banyak pada perempuan (19,5%). Simpulan: Gambaran USG hepar abnormal terbanyak pada kelompok umur 36 – 45 tahun, 46 – 55 tahun, 56 – 65 tahun (23,4%). Gambaran USG hepar terbanyak adalah gambaran USG Fatty Liver (37,7%). Sebaiknya pasien yang datang dengan keluhan seperti nyeri abdomen yang kronik, dan berulang dipastikan penyebabnya melalui pemeriksaan USG abdomen, Untuk membantu mendiagnosis, menyingkirkan kemungkinan kelainan abdomen lainnya dan mencegah memberatnya penyebab nyeri abdomen.Kata kunci: Ultrasonografi Hepar, Penyakit Hepar