THE DEVELOPMENT OF INCLUSIONS IN TISSUE CULTURES OF MONKEY KIDNEY EPITHELIAL CELLS INFECTED WITH POLIOMYELITIS VIRUS

A cytoplasmic inclusion body has been found in the epithelial cells of monkey kidney grown in tissue culture and infected with poliomyelitis virus. This inclusion is at first closely applied to the nucleus, and later develops into a clearly demarcated structure. The nucleus is pushed to the periphery of the cell and becomes pyknotic. Finally, the cytoplasm around the inclusion becomes vacuolated, and the cell breaks up at about the time virus first appears in the fluid part of the infected tissue cultures. Multiple small intranuclear eosinophilic inclusion bodies have also been found in some cells that contain cytoplasmic inclusions.