dual polarisation interferometry
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2020 ◽  
Vol 73 (3) ◽  
pp. 195 ◽  
Author(s):  
Tzong-Hsien Lee ◽  
Kristopher Hall ◽  
Marie-Isabel Aguilar

The binding of melittin to a range of phospholipid bilayers was studied using dual polarisation interferometry and atomic force microscopy. The phospholipid model membranes included zwitterionic dimyristylphosphatidylcholine (DMPC), together with mixtures of DMPC/dimyristylphosphatidylglycerol (DMPG) and DMPC/DMPG/cholesterol. Melittin caused significant disruption on all bilayers, but differences in morphological changes during binding were different on each membrane. Overall, the results demonstrate that the process of membrane disruption follows distinct structural changes for different lipid mixtures irrespective of the strength of binding to the membrane surface.


BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jennifer Payne ◽  
Tzong-Hsien Lee ◽  
Marilyn Anderson ◽  
Marie-Isabel Aguilar

2016 ◽  
Vol 473 (23) ◽  
pp. 4473-4491 ◽  
Author(s):  
Ka Wai Fung ◽  
David W. Wright ◽  
Jayesh Gor ◽  
Marcus J. Swann ◽  
Stephen J. Perkins

During the activation of complement C4 to C4b, the exposure of its thioester domain (TED) is crucial for the attachment of C4b to activator surfaces. In the C4b crystal structure, TED forms an Arg104–Glu1032 salt bridge to tether its neighbouring macroglobulin (MG1) domain. Here, we examined the C4b domain structure to test whether this salt bridge affects its conformation. Dual polarisation interferometry of C4b immobilised at a sensor surface showed that the maximum thickness of C4b increased by 0.46 nm with an increase in NaCl concentration from 50 to 175 mM NaCl. Analytical ultracentrifugation showed that the sedimentation coefficient s20,w of monomeric C4b of 8.41 S in 50 mM NaCl buffer decreased to 7.98 S in 137 mM NaCl buffer, indicating that C4b became more extended. Small angle X-ray scattering reported similar RG values of 4.89–4.90 nm for C4b in 137–250 mM NaCl. Atomistic scattering modelling of the C4b conformation showed that TED and the MG1 domain were separated by 4.7 nm in 137–250 mM NaCl and this is greater than that of 4.0 nm in the C4b crystal structure. Our data reveal that in low NaCl concentrations, both at surfaces and in solution, C4b forms compact TED–MG1 structures. In solution, physiologically relevant NaCl concentrations lead to the separation of the TED and MG1 domain, making C4b less capable of binding to its complement regulators. These conformational changes are similar to those seen previously for complement C3b, confirming the importance of this salt bridge for regulating both C4b and C3b.


Soft Matter ◽  
2011 ◽  
Vol 7 (16) ◽  
pp. 7223 ◽  
Author(s):  
Benjamin J. Cowsill ◽  
Paul D. Coffey ◽  
Mohammed Yaseen ◽  
Thomas A. Waigh ◽  
Neville J. Freeman ◽  
...  

2006 ◽  
Vol 11-12 ◽  
pp. 363-366 ◽  
Author(s):  
Xiao Nong Chen ◽  
Robert Pelton

Polystyrene (PS), polyethylene (PE), polypropylene (PP), glass and stainless steel were exposed to aqueous solutions of a series amphiphilic polymers at room temperature, including N-isopropylacrylamide (NIPAM)-based polymers, polyvinylpyrrolidone (PVP), polypropylene oxide (PPO)-polyethylene oxide (PEO) block copolymers and PEO. Dynamic contact angle measurements of the material surfaces before and after the treatment indicate that only NIPAM-based polymers can adsorb on both hydrophobic and hydrophilic surfaces. The surface morphologies of the materials before and after polymer adsorption were investigated by profilometry. Protein adsorption on the surfaces pre-adsorbed NIPAM-based polymers was investigated by dual polarisation interferometry (DPI) and profilometry using lysozyme as the model protein. The results obtained indicate that NIPAM-based polymers can significantly improve the biofouling resistance of synthetic surfaces.


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