AMP-activated protein kinase — the fat controller of the energy railroadThis paper is one of a selection of papers published in this Special issue, entitled Second Messengers and Phosphoproteins—12th International Conference.

AMP-activated protein kinase plays an important role in the regulation of lipid metabolism in response to metabolic stress and energy demand. It is also under endocrine control. AMPK acts at multiple steps and has a central role controlling fatty acid, triglyceride, and cholesterol synthesis, as well as the oxidation of fatty acids through direct phosphorylation effects and the control of gene transcription. As such, it can be considered to be the fat controller of the energy railroad. It is thought that AMPK may be a major mediator of the health benefits of exercise in mitigating the development of obesity and age-onset diseases.

Comparison of glucose, LCT, and LCT plus MCT as calorie sources for parenterally nourished rats

Protein and fat metabolism were studied in fed and protein-depleted rats. The rats were given one of three isocaloric, isonitrogenous nutrient mixes parenterally. The nutritional regimens differed in the source of nonprotein calories: i) glucose, ii) an emulsion containing long-chain fatty acid triglyceride esters (LCT), and iii) an emulsion containing both LCTs and medium-chain fatty acid triglycerides (MCT). Nitrogen balance, protein synthesis and breakdown, fat deposition in the liver, and the periuterine fat pads were measured using [15N]glycine as the tracer for the protein metabolism and deuterium for the lipid studies. Results are as follows. i) Nitrogen retention and protein synthesis were greater in the fed rats treated with glucose than with LCT. ii) Nitrogen fluxes were lower with LCT than with glucose. iii) Extensive lipogenesis in the liver was only found with the glucose-treated rats. iv) None of the caloric regimens promoted lipogenesis in the periuterine fat pads. v) With the two lipid-containing regimens there was a relative depletion of the depot fat in the periuterine fat pads relative to their glucose-treated counterparts. v) Although the MCT-containing emulsion did not cause hepatomegaly, its apparent caloric effectiveness was lower than that of either glucose or LCT. vi) Chain elongation is not a major pathway for MCT metabolism in parenterally nourished rats.