RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation

The CCR4-NOT complex acts as a central player in the control of mRNA turnover and mediates accelerated mRNA degradation upon HDAC inhibition. Here, we explored acetylation-induced changes in the composition of the CCR4-NOT complex by purification of the endogenously tagged scaffold subunit NOT1 and identified RNF219 as an acetylation-regulated cofactor. We demonstrate that RNF219 is an active RING-type E3 ligase which stably associates with CCR4-NOT via NOT9 through a short linear motif (SLiM) embedded within the C-terminal low-complexity region of RNF219. By using a reconstituted six-subunit human CCR4-NOT complex, we demonstrate that RNF219 inhibits deadenylation through the direct interaction of the α-helical SLiM with the NOT9 module. Transcriptome-wide mRNA half-life measurements reveal that RNF219 attenuates global mRNA turnover in cells, with differential requirement of its RING domain. Our results establish RNF219 as an inhibitor of CCR4-NOT-mediated deadenylation, whose loss upon HDAC inhibition contributes to accelerated mRNA turnover.

Performance characteristics of bistable active ring resonators based on ferrite films

A bistability phenomenon in a ring resonator consisting of a delay line on surface spin waves and a microwave amplifier has been experimentally investigated. It is shown that an enhancement of the gain coefficient above a specified value provides an appearance of a hysteresis at the resonator transmission characteristic. A frequency range of the bistability broadens due to an increase of the gain coefficient as well as the external magnetic field. While the value of the gain is limited from above by a transition of the ring into a self-oscillating regime, an increase in the magnetic field from 1150 Oe up to 3150 Oe provides an expansion of the frequency range of the hysteresis from 77 kHz to 185 kHz.

GEOTHERMAL POTENTIAL ON SUMATRA FAULT SYSTEM TO SUSTAINABLE GEOTOURISM IN WEST SUMATRA

Sumatra Island is an island that is traversed an active ring of fire at Barisan Range which is related to the active Sumatra fault system and geothermal manifestations. It is associated with geothermal manifestations in Cubadak, Talu, Bonjol, and Rimbo Panti, Pasaman Regency, and West Pasaman Regency, West Sumatra Province, as an indication of a geothermal system connected to the Sumatra Fault System from the Sianok Segment and the Talamau Volcano Complex. Sustainable geotourism has become effective for sustainable development of geotourism, the geothermal energy direct utilization. The purpose of this study is to provide sustainable geotourism from geothermal potential in the fault system, taking into account aspects of village geotourism, ecotourism, ecoculture, and education. The methodology used in this study is to collect data on geothermal manifestations from regional geological maps and field observations in geothermal manifestation areas by considering sustainable geotourism. A simple model of sustainable geotourism is made. Study results indicate several locations of potential geothermal manifestations to be used as sustainable geotourism associated with the Sumatra Fault System and the Talamau Volcano Complex.

Single Active Ring Model With Velocity Self-Alignement

Cellular tissue behavior is a multiscale problem. At the cell level, out of equilibrium, biochemical reactions drive physical cell-cell interactions in a typical active matter process. Cell modeling computer simulations...