HISTOPATHOLOGICAL AND IMMUNOFLUORESCENT STUDIES IN CERTAIN SKIN DISORDERS

Background: This study was an attempt to evaluate the role of direct immunofluorescent technique to demonstrate the immunoglobulins in certain skin disorders, which are likely to have immunological mechanism in their pathogenesis. Methods: This study was carried out in department of Pathology, Sardar Patel Medical College & Associated group of Hospitals, Bikaner. This study was hospital based study on skin biopsy specimen recieved in the department of Pathology during the study period. Results: On immunofluorescent studies of these skin biopsies, 12 out of 16 cases of pemphigus were positive for fluorescence was intracellular area in epidermis. In lichen planus 5 out of 7 cases were positive for fluorescence and commonest site was dermo-epideremal junction. In dermatis-herpatiformis 2 out of 3cases were positive for fluorescence and commonest site was dermo-epideremal junction. IgG was commonest type of immunoglobulin’s demonstrate in 12 out of 16 cases of pemphigus followed by IgM (5 cases), IgA(1cases). In lichen planus IgM was commonest type of immunoglobulin’s demonstrate in 5 out of 7 cases of pemphigus followed by IgG (3 cases), IgA(1cases). In dermatitis herpatiformis IgA was commonest type of immunoglobulin’s demonstrate in 2 out of 3 cases of pemphigus followed by IgM (1 cases). Conclusion: We conclude that the demonstration of immunoglobulins in skin biopsies by direct fluorescent technique is a quite useful adjunct in diagnostic confirmation of pemphigus, lichen planus and dermatitis herpetiformis. Keywords: Direct immunofluorescence, Pemphigus, Histopathology.

Autoimmune study in toxoplasmosis women

The effecting of toxoplasmosis in the production of autoantibodies was studied through threemarkers, anti-nuclear antibody (ANA), anti-neutrophil cytoplasmic antibody (ANCA) andrheumatoid factor (RF). The immunofluorescent technique (IFAT) was used to estimate theANA and ANCA, It was found that 9.8% and 5.5% of toxoplasmosis patients had ANA andANCA respectively, but with no significant differences (P>0.05) when comparing with the control group of each one. RF was tested by LAT and revealed that 10% of both infectedwomen and healthy controls had antibodies against RF with no significant differences(P>0.05) between them.