Cellulase Production by Penicillium Oxalicum Ti-11 with Traditional Chinese Medicine Residue as Substrate

PurposeThe study aims to search for efficient cellulase producer and explore the possibility of traditional Chinese medicine residue as a substrate for cellulase production, so as to realize the waste utilization of traditional Chinese medicine residue.MethodsThe cellulase-producing strain was identified through morphological and molecular biological methods. Scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) were used to characterize the structure of traditional Chinese medicine residues before and after fermentation. The enzyme activity was determined by DNS method, and the enzyme production conditions were optimized by single factor and response surface methodology.ResultThe strain grew well in forsythia leaf residue, and the highest FPA could reach 2.06 IU/mL. In addition, the structural characteristics of traditional Chinese medicine residue that before and after enzymatic hydrolysis were characterized by SEM and FTIR. The results showed that the structure of the residue was destroyed after enzymatic hydrolysis, the damage of forsythia leaf residue was the most serious, and enzymatic hydrolysis promoted the dissolution of cellulose, lignin and hemicellulose. The enzyme production conditions of the strain were optimized by Plackett-Burman design and response surface analysis. The FPA could reach 2.79 IU/mL under the optimal conditions of FLR concentration 24.84 g/L, (NH4)2SO4 concentration 2 g/L, temperature 34.44℃, pH 6.20, rotational speed 200rpm, inoculum 6%, which was 35.44% higher than that before optimization.ConclusionsThe results showed that traditional Chinese medicine residue could be used as the induced substrate for fungal cellulase production. This study provides an idea for the low-cost production of fungal cellulase and the waste utilization of traditional Chinese medicine residue.

LAYANAN VIDEO SHARING SOSIAL SEBAGAI PANDUAN PEMANFAATAN LIMBAH NON ORGANIK DAN ORGANIK DI DINAS KEBERSIHAN KOTA KUPANG BERBASIS WEB RESPONSIVE

Waste is a waste product produced by both industrial and domestic production (household), its presence at a certain time and place is undesirable because it has no economic value. Chemically verified, this waste includes organic chemicals (waste that can be decomposed: leaf residue, food particles) and non-norganic (waste that cannot be decomposed: metal, plastic). At certain concentrations and levels, the presence of waste may have a negative impact on the environment, in particular on human health. Therefore, it is necessary to handle the waste. Measures must be taken to reduce the amount of waste generated by use. Video sharing services based on this site is a support solution for the Department of Sanitation of the city of Kupang in dealing with the problems of waste that occur in the community, in particular directly raising awareness of the community to each other to motivate others in the use of waste generated.

Allelopathic Potentiality of Euphorbia hypericifolia L. on Germination and Seedling Development of Sympatric Crops and Weeds

Euphorbia hypericifolia, a non-native herbaceous weedy plant was found to often form disproportionately dense, virtually monospecific stands in the Mount Bambouto Caldera, Cameroon. This observation is within the purview of allelopathy and is attributed to the release of botanicals by non native plants that harm naïve native flora, creating significant problems for agriculture and the environment. We investigated if the invasiveness of E. hypericifolia could be explained by its allelopathic potential at varying concentrations on five indicator plants. The results showed that the leaf, root and stem aqueous extracts of the plant exhibited significant inhibitory effects on the germination, radicle and plumule lengths of Biden pilosa, Amaranthus spinosus, Lactuca sativa, Zea mays and Lycopersicon esculentum at 100 % by 75.0, 69.4, 95.6, 28.0 and 97.2 %, respectively. The leaf extract was the most potent, while the stem extract was the least. The extract of E. hypericifolia was stimulatory (Lower concentrations) and inhibitory to the germination and growth of the test plants and its effect was concentration-dependent with the roots of target plants being more sensitive to the extracts than the plumule. L. esculentum was the most susceptible species to E. hypericifolia extracts while Z. mays was more tolerant than any of the tested plants. Results of total chlorophyll pigment accretion showed declining levels of chlorophyll detected in the leaves of tomato transplants cultivated in the augmented soils with rising dosage of the leaf residue of E. hypericifolia. B. pilosa planted on E. hypericifolia infested soil (EIS) showed very poor emergence recording only 7.5 % after 6 weeks as compared to 83.0 % in non E. hypericifolia infested soil (NIS). Qualitative phytochemical screening indicates E. hypericifolia is rich in secondary metabolites including alkaloids, glycosides, flavonoids, phenolics, tanins, Steriods and saponins. Acetone was the best extractant amongst the solvents used. This study has proven the allelopathic potential of E. hypericifolia and revealed some allelochemicals that may be exploited for the development of bioherbicides and plant growth promotion from natural products.

Feed intake and digestibility in Yankasa sheep fed diets containing various levels of Leucaena leucocephala leaf residues

Four Yankasa sheep, weighing between 19 and 24kg live weight and with age range between 15-24 months were used in a metabolism study to determine the intake and the digestibility of Leucaena leucocephala leaf residue (LLLR) while diets B, C and D had 15, 30 and 45% LLLR inclusion respectively. Dry matter and dietary crude protein intake by trhe environmental animals decreased with increasing level of LLLR inclusion in the diets, although these values were not significant (P>0.05). Also, digestion rates for dry matter and nutrients decreased as protein level of the diets decreased but variation were not significant (P<0.05). Inclusion of 15% LLLR in the dust produced the best weight gain, though this was significantly (P>0.05) different from other treatments. None of the animals developed any health problem and none was underfed as they all recorded an increase in weight. The diet with 15% LLLR produced performance similar to the control both in nutrient intake and weight gain

Insecticidal activity of the extracts of Piper retrofractum fruit and Tephrosia vogelii leaf and their mixtures against Crocidolomia pavonana

This laboratory work was carried out to evaluate the insecticidal activity of the extracts of Piper retrofractum (Piperaceae) fruit and Tephrosia vogelii (Fabaceae) leaf and their mixtures against the cabbage head caterpillar, Crocidolomia pavonana. Ground plant materials of the two plant species were extracted separately with n-hexane and methanol. The results of leaf-residue feeding bioassays showed that P. retrofractum (Pr) and T. vogelii (Tv) hexane extracts had strong insecticidal activity against C. pavonana larvae (LC95 < 0.5%) and were more active than their respective methanol extracts. Pr and Tv hexane extract had a moderate and a rather weak contact effect on C. pavonana larvae, respectively. In feeding tests, Pr + Tv (1:1) hexane and methanol extract mixtures indicated synergistic joint effect both at LC50 and LC95 level, whereas in the contact test, the mixture of Pr + Tv (1:1) hexane extract was synergistic at the LC50 level but antagonistic at the LC95 level. In choice tests, antifeedant effects of Pr and Tv hexane extracts at LC25 to LC70 levels on C. pavonana larvae followed a concentration-dependent fashion. Thus, separate or mixed P. retrofractum and T. vogelii extracts are potential alternatives for the control of C. pavonana.

Nutritional Characteristics of Biochar from Pineapple Leaf Residue and Sago Waste

Biochar produced from biomass with high nutrient content is essential for improving the quality of agricultural soils. An abundance of biomass is converted into biochar with high nutrient content, but studies on the conversion of pineapple and sago waste into biochar are still limited. This research aimed to produce biochar from pineapple leaf (PLB), sago bark (SBB), and sago pith (SPB) through the carbonization process with low temperature. The samples were carbonized using a laboratory electric oven at a low temperature of 350°C. The raw biomass and biochar produced were then subjected to elemental analysis and characterization. The mineral contents of carbonized biochar such as K, N, S, Mg, and Ca increased from those of the feedstock concentrations. For PLP, K element increased 24-fold from 2.44 ± 0.73% to 48.32 ± 9.92%, while N element increased from 6.13 ± 2.39% to 8.33 ± 5.34%. However, for both SBB and SPB, N and K nutrients increased by 2-fold. The study reveals that pineapple leaf biochar has the potentials to be used as an alternative soil amendment to elevate soil nutrient and quality.

Aktivitas Sinergistik Campuran Ekstrak Buah Piper aduncum dan Tiga Jenis Insektisida terhadap Ulat Plutella xylostella

Synergistic Activity of Mixtures of Piper aduncum Fruit Extract and Three Commercial Insecticides against the Diamond Back Moth, Plutella xylostellaPlutella xylostella is an important pest of Brassicaceae vegetable crops. Frequent use of insecticides containing single active ingredients can cause resistance in P. xylostella. This work was done to evaluate the synergism between Piper aduncum fruit extract and three commercial insecticides, i.e. abamectin, chlorfenapyr, and spinetoram, against P. xylostella from Pacet District, Cianjur Regency. Results of leaf-residue feeding bioassays with 48-h feeding treatment showed that LC95 of abamectin, chlorfenapyr, and spinetoram at 96 h after treatment (HAT) was about 5.8, 7.0, and 1.9-fold higher than their respective field rates. Thus, P. xylostella larvae from Pacet-Cianjur were not susceptible to abamectin and chlorfenapyr, but were still fairly susceptible to spinetoram. LC95 of P. aduncum extract at 96 HAT was 0.68% (w/v). Based on combination index at the LC95 level - 96 HAT, mixtures of P. aduncum extract with all three test insecticides were synergistic. Thus, P. aduncum extract is potential to be used as an alternative ingredient to increase the effectiveness of the three test insecticides against P. xylostella.