The Research and Application of System for Paper Surface Morphology Stereoscopic Observation and Characterization

The work aims to explore a microscopic observation system of paper surface and achieve high-precision stereoscopic observation with detail characterization of paper surface morphology. Based on the DT-400E precision program-controlled three-dimensional translation stage and KEYENCE LJV-7200 two-dimensional laser scanner, the hardware parts of our own system are developed to scan and transmit point cloud data of paper surface morphology to the computer. The corresponding system software will automatically process the point cloud data acquired from the laser scanner and generate the corresponding vivid 3D model and height histogram. This system scans and characterizes four different types of paper samples, allowing the human eye to visually distinguish the differences in surface morphology as well as automatically calculate the numerical differences in paper surface morphology parameters. The results of the applicability test show that the system is highly efficient in acquiring, observing, and evaluating the topography of the paper surface. The system can not only predict the paper surface quality of printed paper, but can also be extended to the evaluation of 3D printed surfaces.

Direct Observation of A Large-scale CME Flux Rope Event Arising from an Unwinding Coronal Jet

<p>Increasing observations show that coronal jets may result in bubble-shaped coronal mass ejections (CMEs), but the genesis of jet-driven CMEs and their nature are not fully understood. Here, we report a direct stereoscopic observation on the magnetic coupling from a coronal blowout jet to a stellar-sized CME.  Observations in the EUV passbands of SDO/AIA show that this whole event starts with a small-scale active-region filament whose eruption occurs at a coronal geyser site due to flux emergence and cancellation. By interacting with an overlying null-point configuration, this erupting filament first breaks one of its legs and triggers an unwinding blowout jet. The release of magnetic twist in its jet spire is estimated at around 1.5−2.0 turns. This prominent twist transport in jet spire rapidly creates a newborn large-scale flux rope from the jet base to a remote site. As a result, the newborn large-scale flux rope erupts into the outer coronae causing an Earth-directed bubble-shaped CME. In particular, two sets of distinct flare post-flare loops form in its source region in sequence, indicating this eruptive event couples with twice flare reconnection. This observation highlights a real pathway for jet-CME magnetic coupling and provides a new hint for the buildup of large-scale CME flux ropes.<span> </span></p>

Dynamics of Schistosoma Mansoni Development in The Intermediate Host, Biomphalaria Glabrata, A Freshwater Snail

Background Schistosomiasis is one of the most significant and prevalent waterborne parasitic diseases. Even though many studies have been reported about schistosomiasis, the dynamics of schistosome in intermediate host snails is little known. In the present study, the dynamics of Schistosoma larvae in infected snails was histologically investigated. Methods To examine the localization of Schistosoma mansoni (S. mansoni) parasites in the snails, Biomphalaria glabrata snails infected with miracidia were harvested and examined by stereoscopic observation. Then, frozen sections were prepared and stained with H&E. Furthermore, immunohistochemical detection of parasites was performed using anti-S. mansoni antibody, and their localization in the snails was analyzed. Results Snails infected with S. mansoni miracidia were harvested at 10 and 56 days post-infection (DPI) and analyzed. In the stereoscopic observations, white spots were observed at 56 DPI, while no spots were observed at 10 DPI. However, histological investigations visualized the larvae specifically in the head-foot area of the snail at 10 DPI. Further, it was observed that the larvae relocated to the hepatopancreas and ovotestis areas at 56 DPI. Conclusions The present study revealed the dynamics of Schistosoma larvae in intermediate snails, shown as the differential localization of S. mansoni larvae at early and late infection stages.

97 Loss of aggregation capacity of bovine invitro-produced embryos and blastocyst-derived trophoblasts from Day 6 of development

Embryo aggregation consists of placing more than one zona-free (ZF) embryo in contact during development to obtain a unique structure. It has been reported in different species that aggregated cloned embryos show certain benefits compared with nonaggregated embryos. One way to obtain these benefits in IVF embryos would be to generate a transient chimera by the introduction of trophoblastic cells. Bovine trophoblastic cells can be obtained by embryo bisection of blastocysts, cutting asymmetrically to use trophoblasts (Tr) for aggregation and leaving aside the portion that contains the inner cell mass (ICM). Taking all this into account, the objectives of this work are to study the aggregation of Tr at different days of development and to determine the appropriate time of aggregation. To this aim, cumulus-oocyte complexes (COCs) collected from slaughterhouse ovaries were matured in tissue culture medium 199 containing 10% fetal bovine serum, 10µgmL−1 FSH, 0.3mM sodium pyruvate, 100mM cysteamine and 2% antibiotic-antimycotic for 24h, at 6.5% CO2 in humidified air and 38.5°C. We performed IVF with 16×106 spermatozoa per mL for 5h. Afterwards, presumptive zygotes were cultured in synthetic oviductal fluid (SOF) for 7 days in a humidified atmosphere at 38.5°C, 5% O2, 5% CO2, and 90% N2. In Experiment 1, embryo bisection of Day 7 blastocysts was performed manually under stereoscopic observation with a microblade to obtain Tr. These were aggregated, with the bisected part containing the ICM (n=22) or with ZF embryos of Days 4 (n=23), 5(n=25), or 6 (n=22) and blastocysts (n=25), and placed in microwells in a 100-μL SOF drop covered by mineral oil (Gambini et al. 2012 Biol. Reprod. 87, 15; https://doi.org/10.1095/biolreprod.112.098855). In Experiment 2, ZF synchronous whole embryos were aggregated in microwells at different developmental days: Day 3 (n=18), 4 (n=18), 5 (n=47), 6 (n=48), and 7 (n=45). In both experiments, aggregation was assessed at Day 8. In Experiment 1, no aggregation was observed between the Tr and the embryos or the bisected ICM. Experiment 2 showed embryo aggregation on Days 3 (55%), 4 (27%), and 5 (61%), whereas on Days 6 and 7 no aggregation was observed. According to these results, we can conclude that, in our culture conditions, Tr obtained by blastocyst bisection have no capacity for aggregation. Day 6 and 7 whole ZF embryos also do not aggregate. As a general conclusion, there is a period from Days 0-5 of the invitro development of bovine embryos in which aggregation is possible. Aggregation of blastocyst-derived Tr to cloned or high-value IVF embryos, aiming for quality improvement, is not an effective strategy.

VALIDATION OF "AW3D" GLOBAL DSM GENERATED FROM ALOS PRISM

Panchromatic Remote-sensing Instrument for Stereo Mapping (PRISM), one of onboard sensors carried by Advanced Land Observing Satellite (ALOS), was designed to generate worldwide topographic data with its optical stereoscopic observation. It has an exclusive ability to perform a triplet stereo observation which views forward, nadir, and backward along the satellite track in 2.5 m ground resolution, and collected its derived images all over the world during the mission life of the satellite from 2006 through 2011. A new project, which generates global elevation datasets with the image archives, was started in 2014. The data is processed in unprecedented 5 m grid spacing utilizing the original triplet stereo images in 2.5 m resolution. As the number of processed data is growing steadily so that the global land areas are almost covered, a trend of global data qualities became apparent. This paper reports on up-to-date results of the validations for the accuracy of data products as well as the status of data coverage in global areas. The accuracies and error characteristics of datasets are analyzed by the comparison with existing global datasets such as Ice, Cloud, and land Elevation Satellite (ICESat) data, as well as ground control points (GCPs) and the reference Digital Elevation Model (DEM) derived from the airborne Light Detection and Ranging (LiDAR).

VALIDATION OF "AW3D" GLOBAL DSM GENERATED FROM ALOS PRISM

Panchromatic Remote-sensing Instrument for Stereo Mapping (PRISM), one of onboard sensors carried by Advanced Land Observing Satellite (ALOS), was designed to generate worldwide topographic data with its optical stereoscopic observation. It has an exclusive ability to perform a triplet stereo observation which views forward, nadir, and backward along the satellite track in 2.5 m ground resolution, and collected its derived images all over the world during the mission life of the satellite from 2006 through 2011. A new project, which generates global elevation datasets with the image archives, was started in 2014. The data is processed in unprecedented 5 m grid spacing utilizing the original triplet stereo images in 2.5 m resolution. As the number of processed data is growing steadily so that the global land areas are almost covered, a trend of global data qualities became apparent. This paper reports on up-to-date results of the validations for the accuracy of data products as well as the status of data coverage in global areas. The accuracies and error characteristics of datasets are analyzed by the comparison with existing global datasets such as Ice, Cloud, and land Elevation Satellite (ICESat) data, as well as ground control points (GCPs) and the reference Digital Elevation Model (DEM) derived from the airborne Light Detection and Ranging (LiDAR).