Rats were trained to traverse a straight alley for either 1 or 10 food pellets as reward. Each runway trial was preceded by an intertrial reinforcement of 1 or 10 pellets. A negative contrast effect obtained and persisted throughout the 72 trials conducted. Although there was a strong suggestion of a positive contrast effect throughout training, the effect did not prove reliable. The contrast effect results were interpreted as challenging the view that with extended training ITRs become “irrelevant” to runway reward.