Fermenting Feminism as Methodology and Metaphor

This article proposes the possibilities of fermentation, or microbial transformation, as a material practice and speculative metaphor through which to approach today’s transnational feminisms. The author approaches this from the perspective of their multiyear curatorial experiment Fermenting Feminism, looking to multidisciplinary practices across the arts that bring together fermentation and feminism in dynamic ways. The article outlines ten ways in which fermentation is a ripe framework for approaching transinclusive, antiracist, countercolonial feminisms. As the author takes up these points, drawing from scholarly and artistic references alongside lived experience, they theorize the ways fermentation taps into the fizzy currents within critical and creative feminist practices. With its explosive, multisensory, and multispecies resonances fermentation becomes a provocation for contemporary transnational feminisms. Is feminism, with its etymological roots in the feminine, something worth preserving? In what ways might it be preserved, and in what ways might it be transformed? The author proposes that fermentation is a generative metaphor, a material practice, and a microbiological process through which feminisms might be reenergized—through symbiotic cultures of feminisms, fermentation prompts fizzy change with the simultaneity of preservation and transformation, futurity and decay.

Design for Software Risk Management Using Soft Computing and Simulated Biological Approach

The process of software evaluation is one of the key tasks that are addressed by the quality assurance teams so that the risks in the software suite can be identified and can be removed with prior notifications. Different types of metrics can be used in defect prediction models, and widely used metrics are source code and process metrics. A simulated environment for the entire process shall be generated for multiple scenarios and parameters so that the results and conclusion can be depicted in an effective way. The focus of research is to develop a narrative architecture and design for software risk management using soft computing and nature-inspired approach. The proposed approach titled simulated biological reaction (SBR) is expected to have the effectual results on multiple parameters with the flavor of soft computing-based optimization. The proposed approach shall be integrating the simulation of microbiological process in different substances and elements to produce a new substance.

Mathematical modeling of processes of interaction of functional and which contaminate microorganisms in biotechnology system

Mathematical modeling of processes occurring in the biotechnological system, including those describing the interaction of functional microorganisms with contaminating microflora, is currently a promising scientific direction. The article discusses the issues of system analysis of the processes occurring in the biotechnological system during fermentation. By "microbiological system" is meant a microbiological process consisting of two subsystems or populations of microorganisms. During a given technological process, these populations interact with each other by using a common resource. Systems analysis and mathematical modeling of microbiological processes is a complex task. It is necessary to take into account a lot of factors, such as the simultaneous occurrence of several non-stationary processes; many state parameters, including analysis of the links between them; change in real time of technological parameters, in particular, the main resource, consideration of various properties of microbial populations affecting the behavior of microbiological systems (mortality, fertility, the existence of extraneous microflora, etc.), and other factors that have a direct impact on the quality of the finished product. In turn, a quantitative analysis of these interactions will lead to the development of scientifically based methods and approaches that minimize the effect of contamination. The analysis was carried out and a system model was constructed reflecting the entire spectrum of interactions between useful and extraneous populations of microorganisms in the production of bakery products. The presented system model allows to continue the study of the questions posed in the work, by further studying its components.

Biogenic factors of geochemical uranium anomalies formation in subsurface waters near to Novosibirsk chemical concentrates plant slurry storage

This article presents data on the possible impact of aerobic and anaerobic microbiological processes in the upper aquifers to uranium migration t sulfate and nitrate polluted waters near to Novosibirsk chemical concentrate plant. Uranium immobilization is possible in local areas, with high content of organic substances and the most important microbiological process is the redox potential reduction due to aerobic respiration. After that in anaerobicс conditions redox-dependent uranium reduction can be expected. Moreover, in the presence of sulfate ions, further anaerobic processes of microbial sulfate reduction and iron reduction lead to the formation of iron sulphide, which plays the significant role of an antioxidant buffer in the case of oxygen migration.

Microbiological parameters of cheese paste with the content of flaxseed oil at different storage temperatures

The purpose of the work was to investigate the effect of storage temperature on the change of microbiological parameters of experimental samples of cheese paste with different contents of flaxseed oil. It was established that storage of cheese paste with different contents of flaxseed oil at a temperature of + 2 ± 0.5 °С for 14 days did not cause a probable increase in lactic acid microorganisms, compared with their initial amount and a control sample – sour milk cheese. The titer of coliform, the content of mold fungi and yeast during 14 days of storage of experimental samples of sucrose paste gradually increased, but did not exceed the maximum permissible values according to National standard of Ukraine. Provision of normative microbiological indicators of quality and safety of cheese paste with different contents of flaxseed oil, during its fourteen-day storage at a temperature of + 2 ą 0,5 °С, is possible under strict hygiene requirements during production. It was found that the temperature of 6 ± 1 °С, to a lesser extent, slows down the microbiological process during storage of experimental samples of cheese paste and dairy cheese compared to a temperature of 2 ± 0.5 °С. During the 14 days storage, the lactic acid process was intensified, resulting in an increase in lactococcal content, approximately 30 times, and amounted to 8.87 lg CFU/g of the product. It was established that after seven days of storage in all experimental samples of whey germ, coliforms was detected in 0.1 g of the product i.e. their titre decreased by one. However, for such a value of the titres of coliforms, the experimental samples of the cheese paste met the requirements of the State Standard of Ukraine. The next seven-day storage of samples of sucrose paste at a temperature of 6 ± 1 °C caused an increase in the number of coliforms and their titre exceeded the normative value of 0.01 g. Fungi and yeast in samples of cheese paste during storage at a temperature of 6 ± 1 °С multiplied, their amount during seven days of storage increased in 3.9–5.4 times. However, due to their insignificant initial content in the freshly prepared product, for seven days storage of the amount of fungi and yeast did not exceed the maximum permissible concentration according to National standard of Ukraine. Consequently, experimental samples of cheese paste can be stored at a temperature of 2 ± 0.5 °C for 14 days without significant changes in microbiological parameters, provided there is a minimum amount of them in a freshly prepared product. At the same time, at a temperature of 6 ± 1 °С storage of samples of cheese paste with flaxseed oil is possible without exceeding the normative values of microbiological indices only for 7 days

Predominance of CTX-M Type Extended Spectrum β-lactamase (ESBL) Producers Among Clinical Isolates of Enterobacteriaceae in a Tertiary Care Hospital, Kathmandu, Nepal

Background:ESBL problem is increasing worldwide and only limited studies on genes of ESBL are performed in Nepal.Objectives:We aimed to focus on the molecular detection of plasmid-mediatedblaTEM,blaSHVandblaCTX-Mgenes among the ESBL producing Enterobacteriaceae from different clinical samples.Methods:A total of 550 clinical samples were processed and organisms of Enterobacteriaceae were identified using standard microbiological process. ESBL producers were screened and confirmed using modified Kirby Bauer disc diffusion method by CLSI guidelines. Plasmids extracted from the confirmed ESBL positives were the template for PCR.blaSHV,blaTEMandblaCTX-Mgenes were amplified using specific primers of respective genes by uniplex PCR. The presence of these genes was confirmed by gel electrophoresis.Results:Among 550 different clinical samples 343 (62.36%) were culture positive. Of which, 157 (45.57%) belonged to Enterobacteriaceae.Escherichia coli(45.9%) was predominant. Of these 33.2% (52/157) isolates ESBL positive. ESBL-E. coli(52.8%) were prevalent. All ESBL positive organisms were sensitive to imipenem. Of confirmed ESBL positives, 34.6% harboringblaTEMgene, 30.8% harboringblaSHVgene andblaCTX-Mgenes were present in all ESBL producers. Twenty-eightout of 52 (53.9%) isolates harbored multipleblagenes, the most common combination beingblaCTX-M+blaTEM(21.2%).Conclusion:We report 100% plasmid mediated CTX-M genotype among ESBL producers which might indicate rapid dissemination ofblaCTX-Mgenes from the community to the patients. Besides, there is a need for regular monitoring of antibiotic resistance in the country and de-escalate the use of antibiotics so as to preserve the antibiotics for future generation.

Biodegradation of Hexavalent Chromium from Paint Industry Effluent by Indigenous Bacteria

Hexavalent chromium (Cr-VI) is toxic, mutagenic and carcinogenic chemical, whereas its reduced trivalent form (Cr-III) is much less toxic. Cr-VI is widely used in paint industry, tannery industry, and so on. In the present study an attempt was made to isolate naturally occurring bacteria from paint industry effluent possessing high potentiality to reduce Cr-VI. Seven efficient chromium reducing bacterial strains were isolated as Bacillus korlensis, Bacillus subtilis, Bacillus cereus, Micrococcus luteus, Micrococcus varians, Enterobacter intermedius and Tatumella terrea. These bacteria reduced chromium in culture media at maximum 5 mM concentration within a period of 24–72 h as determined by 1, 5-diphenylcarbazide (DPC) colorimetric method. However, significant Cr-VI reduction or biodegradation was observed at 1.25 mM substrate concentration within 24 h at 37°C. The research was very promising for development of a microbiological process to be used in the removal of toxic hexavalent chromium from the environment.