A Three-Dimensional Coupled Hydrodynamic-Ecological Modeling to Assess the Planktonic Biomass in a Subalpine Lake

In this study, a coupled three-dimensional hydrodynamic-ecological model was developed to comprehensively understand the interaction between the hydrodynamics and ecological status of a lake. The coupled model was utilized to explore the hydrodynamics, water quality, and ecological status in an ecologically rich subalpine lake (i.e., Tsuei-Feng Lake (TFL), located in north-central Taiwan). The measured data of water depth, water temperature, water quality, and planktonic biomass were gathered to validate the coupled model. The simulated results with a three-dimensional hydrodynamic and water quality-ecological model reasonably reproduced the variations in observed water depth, water temperature, water quality, and phytoplankton and zooplankton biomass. Sensitivity analysis was implemented to determine the most influential parameter affecting the planktonic biomass. The results of sensitivity analysis indicated that the predation rate on phytoplankton (PRP) significantly affects the phytoplankton biomass, while the basal metabolism rate of zooplankton (BMZ) importantly affects the zooplankton biomass. Furthermore, inflow discharge was the most important environmental factor dominating the phytoplankton and zooplankton biomass of TFL. This implies that the runoff in the catchment area caused by rainfall and the heavy rainfall induced by climate change may affect the planktonic biomass of the lake.

Unravelling climate change impacts from other anthropogenic influences in a subalpine lake: a multi-proxy sediment study from Oberer Soiernsee (Northern Alps, Germany)

Mountain lakes are increasingly impacted by a series of both local and global disturbances. The present study reveals the eutrophication history of a remote subalpine lake (Oberer Soiernsee, Northern Alps, Germany), triggered by deforestation, alpine pasturing, hut construction, tourism and atmospheric deposition, and identifies the intertwined consequences of on-going global warming on the lake’s ecosystem. The primary objective was to disentangle the various direct and indirect impacts of these multiple stressors via down-core analyses. Our multi-proxy approach included subfossil diatom assemblages, carbon and nitrogen stable isotope ratios and subfossil pigments from dated sediments. Shifts within the diatom assemblages were related to variations in trophic state, lake transparency, water temperature and thermal stratification. The organic carbon isotope (δ13Corg) records, the diatom valve density and the pigment concentrations documented the development of primary production and composition. Total nitrogen isotope values (δ15N) are more likely to reflect the history of atmospheric nitrogen pollution than lake-internal processes, also mirrored by the decoupling of δ15N and δ13Corg trends. The composition of sedimentary pigments allowed a differentiation between planktonic and benthic primary production. Concordant trends of all indicators suggested that the lake ecosystem passed a climatic threshold promoted by local and long-distance atmospheric nutrient loadings.

Effects of Seasonal Freezing-thawing on the Protistan Communities in a Mountain Lake

Background: Freezing-thawing cycles are common phenomena in temperate regions. Such events may have a significant influence on the composition of the protistan communities in a mountain lake. Protists are single-celled eukaryotic microorganisms that act as links in the aquatic microbial food web, affecting the transfer of substances and energy transformation. Yet little is known about the effects of freezing and thawing on the protistan community in a mountain lake. Results: The protistan communities in the lake were mainly composed of Ochrophyta, Ciliophora, Choanoflagellida, Cryptophyta, Chlorophyta, Stramenopiles_X, Cercozoa, Dinophyta, and Haptophyta. Seasonal freezing and thawing affected the community composition and diversity of protists. The change in the protistan community structure resulted from a significant change in organic carbon from the ice-covered to ice-free period. During the ice-covered and ice-free periods, temperature and nitrate were the main causes, respectively, for the changes in protistan community structure at different depths. Water depth also affected the structure of the protistan community, but it was not the most important factor. Conclusions: This study revealed that duration of lake surface icing might affect the function of subalpine lake ecosystems, including the rate of nutrient cycling and energy flow, owing to changes in the structure and biodiversity of the microbial community.

Modeling the hydrodynamics of a wetland under strong anthropic pressures (Torbiere del Sebino, Italy)

<p>The Natural Reserve “Torbiere del Sebino” is situated on the southern bank of Lake Iseo and is one the most meaningful wet zone for extension and ecological importance of northern Italy, belonging to the Natura2000 network.</p><p>Torbiere occupies an area of 3.60 km<sup>2</sup> within a 14 km<sup>2</sup> watershed where almost 12000 inhabitants live and where agricultural activities, mostly vineyards, cover almost 40% of the area; this leads to a significant anthropic pressure that over the last 50 years has compromised the system and changed the equilibria between species, enhancing eutrophication.</p><p>Despite the ecological relevance of the area, one of the most important in northern Italy, very little quantitative information is available regarding its current state and evolution in terms of water quality and hydrodynamics.  Given the critical environmental condition of the habitat, it is necessary to address the consequences of human impact on the trophic state of Torbiere.</p><p>Torbiere consists of a system of shallow lakes or ponds (average depth 1.5 m) whose main affluent is a creek (called Rì) entering from the South. A secondary occasional affluent enters the system from the East and consists of a combined sewer overflow (CSO). Finally, the main effluent is an artificial channel located in the North connecting Torbiere directly with the subalpine Lake Iseo. Although originally subdivided into a set of many interconnected ponds, the separation levees have been demolished over the last decades to enhance internal circulation, under the assumption that this would decrease the residence time and improve the water quality. However, no rational argument was used to support this decision that led to a system where similar characteristics (Secchi’s depth, turbidity, specific conductivity) are found all over the study area and where the expansion of invasive species was easier; now there is some evidence that a separate set of ponds would be better manageable to contrast the eutrophication process. To understand this process, a 3D hydrodynamic model has been set up using Delft-3D, an open source, finite difference package. </p><p>Given the great extension of the system, the inner circulation of the water is not driven by the momentum of the affluents, instead the wind plays a major role. This forcing term presents a daily pattern: it blows from the North in the mornings and shifts to the opposite direction in the late afternoon. The water mainly flows from the South to the North. However, preliminary results by Delft 3D showed that the circulation is made complex by the wind. The model shows that opposite directions of horizontal flow velocities are found at the surface and at the bottom of the water column, showing that only the upper layers follow the direction of the wind.</p><p>By comparing the actual and previous conditions of separation of the ponds, the model aims to give an answer to whether the choice of demolishing the banks was positive or negative for the water quality of Torbiere. Once the role of the banks will be clarified, the effects of their possible restoration will be addressed.</p>

Exceptionally rich sedaDNA record from a subalpine lake in the Central Alps reveals plant responses to climate change and human land use

<p>The increase in plant species richness in the Alps over the last century has been described as a direct response to climate warming. Alpine ecosystems are expected to have an upward displacement of vegetation, resulting in shifts of species ranges, high replacement rates, and species loss. To apply proper management measures, it is necessary to understand how drivers of change affect species and ecosystem tipping points. Palaeoecological studies allow us to understand how species responded to similar situations in the past. However, such studies are often challenged by proxy preservation and taxonomic resolution. Metabarcoding approaches based on sedimentary ancient DNA (sedaDNA) can overcome these caveats. </p><p>Here we use plant sedaDNA and the new PhyloAlps taxonomic reference database, which covers 4500 plant taxa from the Alps, to explore alpine floral diversity of Sulseewlii, a subalpine lake in the Central Alps (Switzerland). We present a 12,000 year record of vegetational composition and structural changes in a subalpine ecosystem. To disentangle the relationship between climate and vegetation, we used a novel local temperature reconstruction inferred from chironomids of the same lake. We also used coprophilous fungal spores and charcoal, together with pastoral and arable indicators, to infer human pressure. </p><p>With 377 identified taxa, including 140 at species level, Sulseewlii has yielded the richest dataset of plant sedaDNA to date and emphasizes the Alps as an important biodiversity hotspot in Europe. Out of the identified taxa, 91 are indicators that allowed us to reconstruct the vegetation stages and altitudinal shifts of the main vegetation groups. Total taxonomic richness increases from the onset of the Holocene and has a similar pattern to subalpine-montane DNA taxa, with three marked drops at 8200, 3200, and ~500 cal yr BP. Plant sedaDNA registered a marked transition from alpine to subalpine communities at the onset of the Holocene. The highest proportions of montane taxa occurred during the Holocene climatic optimum (9,000-6,000 cal yr BP). Finally, a shift back to subalpine-montane species with some lowland influence occurs as we approach the present.</p>

Trophic Niches, Trophic Positions, and Niche Overlaps between Non-Native and Native Fish Species in a Subalpine Lake

In the last century, Italian freshwater ecosystems have been invaded by several non-native fish species. In the subalpine Lake Mergozzo (northern Italy), several recently introduced non-native species dramatically expanded their populations. We used carbon and nitrogen stable isotopes to describe the isotopic niches and trophic positions of native and non-native fish species in Lake Mergozzo. We evaluated their trophic niches, trophic diversity, trophic redundancy and trophic evenness utilizing isotopic niche metrics, and estimated asymmetrical niche overlaps. The trophic traits of non-native fish species and Perca fluviatilis clearly define them as trophic generalists, in terms of among-individual variability of their isotopic niches. The historical increase in abundance of fish non-native species in this lake, their dominance by numbers and biomass within the assemblage, and their broad asymmetrical niche overlaps suggest that their higher degree of trophic generalism might have been one of the key factors that have promoted the invasion of the recipient community.

Moored observations of turbulent mixing events in deep Lake Garda, Italy

Deep water circulation and mixing processes are responsible for the transport of matter, nutrients and pollutants in deep lakes. Nevertheless, detailed continuous observations are rarely available. To overcome some of these deficiencies and with the aim of improving our understanding of deep mixing processes, a dedicated yearlong mooring comprising 100 high-resolution temperature sensors and a single current meter were located in the deeper half of the 344 m deepest point of the subalpine Lake Garda, Italy. The observations show peaks and calms of turbulent exchange, besides ubiquitous internal wave activity. In late winter, northerly winds activate episodic deep convective overturning, the dense water being subsequently advected along the lake-floor. Besides deep convection, such winds also set-up seiches and inertial waves that are associated with about 100 times larger turbulence dissipation rates than that by semidiurnal internal wave breaking observed in summer. In the lower 60 m above the lake-floor, however, the average turbulence dissipation rate is approximately constant in value year-around, being about 10 times larger than open-ocean values, except during deep convection episodes.

Model simulations of the ecological dynamics induced by climate and nutrient load changes for deep subalpine Lake Maggiore (Italy/Switzerland)

Climate warming affects lake ecosystems both through its direct effect on the phenology of species and through the alteration of the physical and chemical environments, which in turn affect community composition. In deep lakes, stratification enhancement and mixing reduction have already been observed, leading to hypolimnetic anoxia and to the rise of cyanophytes. The increase in stability depends on the rise of air temperature due to global emissions of greenhouse gases (GHG). Primary production could then either increase with rising epilimnetic temperature and buoyancy or decrease as fewer nutrients are upwelled from deep layers. The prevailing outcome, as well as the quantitative and temporal dynamics of all climate-induced modifications, depend on the specific lake characteristics. Individual analyses are then needed, one-dimensional coupled hydrodynamic-ecological numerical models being suitable tools for such predictions. Here, we simulated with GLM-AED2 (General Lake Model – Aquatic EcoDynamics) the 2020-2085 dynamics of the oligomictic and oligotrophic deep subalpine Lake Maggiore (Italy/Switzerland), according to the Swiss Climate Change Scenarios CH2011. Multiple realisations were performed for each scenario with random meteorological series obtained from the Vector-Autoregressive Weather Generator (VG), highlighting the uncertainties related to meteorology. Increase and decrease of nutrient loads were also tested. Results show that anoxia would occur in the hypolimnion regardless of nutrient input reduction, unless global GHG emissions were immediately reduced. Total phytoplankton biomass would be weakly affected by climate change, strongly depending on nutrient input, yet water warming would cause cyanophytes to compete with diatoms. Therefore, the fate of Lake Maggiore would be tied to both global and local environmental policies.

First Occurrence of Eustrongylides spp. (Nematoda: Dioctophymatidae) in a Subalpine Lake in Northwest Italy: New Data on Distribution and Host Range

The genus Eustrongylides includes nematodes that infect fish species and fish-eating birds inhabiting freshwater ecosystems. Nematodes belonging to the genus Eustrongylides are potentially pathogenic for humans; infection occurs after the consumption of raw or undercooked fish. In the two-year period 2019–2020, a total of 292 fish belonging to eight species were examined for the occurrence of Eustrongylides spp. from Lake San Michele, a small subalpine lake in northwest Italy. The prevalence of infestation was 18.3% in Lepomis gibbosus, 16.7% in Micropterus salmoides, and 10% in Perca fluviatilis. The other five fish species (Ameiurus melas, Ictalurus punctatus, Squalius cephalus, Carassius carassius, and Scardinius erythrophthalmus) were all negative for parasite presence. There were no significant differences in prevalence between the three fish species (Fisher’s exact test; p = 0.744). The mean intensity of infestation ranged from 1 (M. salmoides and P. fluviatilis) to 1.15 (L. gibbosus), and the mean abundance ranged from 0.1 (P. fluviatilis) to 0.28 (L. gibbosus). There were significant differences in the infestation site between the four muscle quadrants (anterior ventral, anterior dorsal, posterior ventral, and posterior dorsal) and the visceral cavity (Kruskal–Wallis test; p = 0.0008). The study findings advance our knowledge about the distribution and host range of this parasite in Italy.