Tissue array analysis in patients with renal cancer.

450 Background: Development and progression of kidney cancer is the result of an imbalance in signals promoting and inhibiting cellular proliferation and apoptosis. The aim of this study is to analize the expression of some molecular regulators and correlate them with clinical outcome in renal carcinoma, in order to establish common prognostic biomarkers. Methods: Tissue sample was obtained from nephrectomized patients in our center between 1998 and 2003. Biopsies were taken from 150 patients; a tissue array was done to analyze immunoexpression of proteins related to proliferation, cell cycle control and angiongenesis: p53, Ki 67, VEGF, VEGFR1, VEGFR2, HIF1, HIF2, cyclin D1, cyclin D3, cyclin B1, cyclin A, bcl2, p63, COX2, p16, p27, p21, EGFR, PDGF, PTEN, CAIX, BRAF, Akt and P13K. Subsequently the results were correlated with clinical variables (age, hypertension, smoking), pathological (tumor size, stage, Fuhrman grade) and time to progression and overall survival. Results: 92.3% of patients underwent nephrectomy, 11% associated with lymphadenectomy. Histology: 66.6% clear cell his, 14.1% papillary, chromophobe 6.4% and 15% miscellaneous. Stage I 49.4%; II 17.9%; 14.7% III; IV 14.1%; 3.8% unknown. Of the 150 patients analyzed, 23% had a second tumor. Median time to progression was 90.5 months (95% CI 81.7 to 114.3). The median survival has not yet been reached. Results of tissue array analysis wil be presented in the meeting. Conclusions: Patients in this serie have a good prognosis, since most tumors are stage I and II. We will present data on the expression of factors related to proliferation, cell cycle control and angiogenesis.