Enzymatic preparation of structured TAG containing conjugated linoleic acid (CLA) at solvent-free

Rice bran oil extracted by the water-enzymatic method was placed at a temperature of −5 °C for 8 h. Light-phase rice bran oil with an iodine value of 112.13 ± 0.21 g I2/100 g oil was separated by cryogenic freezing centrifugation. Lipozyme RM IM transesterified light-phase rice bran oil and conjugated linoleic acid (CLA) under supercritical system CO2 (SC-CO2). The optimal reaction conditions for transesterification of CLA and rice bran oil in SC-CO2 were determined as follows: the Lipozyme RM IM dosage was 9%, and the RBO/CLA molar ratio was 1:3, the reaction temperature was 55 °C, stirring speed was 300 rpm, and transesterification time was 20 h, the CLA conversion rate can reach 42.1%.