elongation product
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2015 ◽  
Vol 104 ◽  
pp. 23-30 ◽  
Author(s):  
Xiao-Dong Tan ◽  
Yun-Bo Xu ◽  
Xiao-Long Yang ◽  
Zhi-Ping Hu ◽  
Fei Peng ◽  
...  

2010 ◽  
Vol 299 (5) ◽  
pp. R1306-R1316 ◽  
Author(s):  
Christopher J. Pynn ◽  
M. Victoria Picardi ◽  
Tim Nicholson ◽  
Dorothee Wistuba ◽  
Christian F. Poets ◽  
...  

Lung surfactant mainly comprises phosphatidylcholines (PC), together with phosphatidylglycerols and surfactant proteins SP-A to SP-D. Dipalmitoyl-PC (PC16:0/16:0), palmitoylmyristoyl-PC (PC16:0/14:0), and palmitoylpalmitoleoyl-PC (PC16:0/16:1) together comprise 75–80% of surfactant PC. During alveolarization, which occurs postnatally in the rat, PC16:0/14:0 reversibly increases at the expense of PC16:0/16:0. As lipoproteins modify surfactant metabolism, we postulated an extrapulmonary origin of PC16:0/14:0 enrichment in surfactant. We, therefore, fed rats (d19–26) with trilaurin (C12:03), trimyristin (C14:03), tripalmitin (C16:03), triolein (C18:13) or trilinolein (C18:23) vs. carbohydrate diet to assess their effects on surfactant PC composition and surface tension function using a captive bubble surfactometer. Metabolism was assessed with deuterated C12:0 (ω-d3-C12:0) and ω-d3-C14:0. C14:03 increased PC16:0/14:0 in surfactant from 12 ± 1 to 45 ± 3% and decreased PC16:0/16:0 from 47 ± 1 to 29 ± 2%, with no impairment of surface tension function. Combined phospholipase A2 assay and mass spectrometry revealed that 50% of the PC16:0/14:0 peak comprised its isomer 1-myristoyl-2-palmitoyl-PC (PC14:0/16:0). While C12:03 was excluded from incorporation into PC, it increased PC16:0/14:0 as well. C16:03, C18:13, and C18:23 had no significant effect on PC16:0/16:0 or PC16:0/14:0. d3-C14:0 was enriched in lung PC, either via direct supply or via d3-C12:0 elongation. Enrichment of d3-C14:0 in surfactant PC contrasted its rapid turnover in plasma and liver PC, where its elongation product d3-C16:0 surmounted d3-C14:0. In summary, high surfactant PC16:0/14:0 during lung development correlates with C14:0 and C12:0 supply via specific C14:0 enrichment into lung PC. Surfactant that is high in PC16:0/14:0 but low in PC16:0/16:0 is compatible with normal respiration and surfactant function in vitro.


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