AB0788 MARKER OF CARTILAGE TISSUE LESION IMMUNOPATHOGENESIS OF RHEUMATOID ARTHRITIS

Background:Collagen type 2 is the basic protein of cartilaginous tissue composing over 80% of its mass. Finding excessive antibodies to collagen type 2 and immune complexes is a diagnostic and prognostic criterion of immune lesion of articular cartilage.Objectives:To study production of antibodies to collagen type 2 in patients with rheumatoid arthritis using immobilized magnetically controlled forms.Methods:The antigen was represented by commercial formulation of collagen type 2 produced by Serva (Sweden). Antibodies to collagen type 2 were determined in the patients’ blood serum by way of a technique of immunoenzyme assay (ELISA test) using immobilized magnetic sorbents. Magnetic sorbents were polyacrylamide granules from 10 to 100 micron in size containing magnetic material and collagen type 2. We studied 30 apparently healthy donor individuals and 92 patients with a confirmed diagnosis of rheumatoid arthritis.Results:A study of sera from rheumatoid arthritis patients revealed antibodies to collagen type 2 in 63 patients (68.48%). The correlation coefficient between anti-collagen type 2 antibodies, and IgA / IgM amounted to 0.28 and 0.36, correspondingly. At stage 1 and 2 of disease activity the level of antibodies was higher than in donors (р<0.001). The highest level of antibodies to collagen type 2 was seen in patients with stage 3 of disease activity (0.55±0.02). The amount of antibodies to collagen type 2 in patients with visceral manifestations of rheumatoid arthritis was no different from that in patients without any additional organ involvement, which is hardly surprising since collagen type 2 is mostly localized in articular cartilage and is practically absent from the connective tissue of other organs and systems.Conclusion:Thus the presence of antibodies to collagen type 2 correlates with the disease activity and is an important marker of articular cartilage lesion in patients with rheumatoid arthritis.Disclosure of Interests:None declared

Montmorillonite of the Ohlanlynske deposit and magnetic composites based on it — sorbents for water purification from 137Cs and 90Sr

A comparative study on the extraction of 137Cs and 90Sr from aqueous media using magnetic composites obtained on the basis of bentonite from the Ohlanlynske deposit and magnetite was carried out. It is shown that magnetic composites based on a natural mineral — bentonite rock, isolated montmorillonite in the Na-form, impurities — clinoptilolite and cristobalite, as well as natural minerals without magnetite, efficiently sorb 137Cs and 90Sr in a wide pH range (3.7–9). Despite the fact that magnetite in the composition of composites practically does not affect the efficiency of purification of aqueous media, but acts as only a magnetoactive component, composites are quite promising sorbents for purifying of large volumes of radioactively contaminated waters due to the possibility of removing water purification sludge using an external magnetic field. It has been established that the highest sorption properties are possessed by magnetic sorbents obtained on the basis of clinoptilolite — for 137Cs, and the Na-form of montmorillonite — for 90Sr. The values of their limiting adsorption by the indicated composites, calculated using the Langmuir equation, are 43.3 and 40.6 mg/g, respectively. It is shown that among the macrocomponents (Na+ , K+ , Ca2+), Ca2+ ions have the greatest influence on the efficiency of purification of natural waters and liquid radioactive waste containing 137Cs and 90Sr radionuclides.

Adsorption Properties of Magnetic Sorbent Mn0.25Fe2.75O4@SiO2 for Mercury Removal

Mercury pollution through water causes several dangerous diseases. Various efforts have been made to reduce mercury pollution. One of them is by using sorbent. Many ways to improve absorption efficiency, one of which is using magnetic sorbents. This study focused on the effect of grain size and the concentration of Mn0.25Fe2.75O4@SiO2 core-shell on mercury absorption efficiency. The synthesis of Mn0.25Fe2.75O4@SiO2 with 6 and 8 mL of TEOS was carried out through coprecipitation and sol-gel methods. The characterization using XRD, VSM, and FTIR was conducted to determine grain size, properties, and material functional groups proving that SiO2 was successfully covered on the Fe3O4 surface. The percentage of absorption was found by using the AAS instrument. Diffraction data confirmed the presence of Fe3O4 and the amorphous SiO2 phase. According to the Rietveld analysis of all samples demonstrated the particle size of Mn0.25Fe2.75O4 around 11-12 nm. The Mn0.25Fe2.75O4 core had superparamagnetic properties for magnetic separation, and the SiO2 shell could protect the core of being oxidized or dissolved under acid condition. FTIR results showed the sample had a functional group of the main components of Fe-O and SiO2 at a wavenumber of 420-507 cm-1 and 801 cm-1 (stretching) and 1078 cm-1 (bending), respectively. The results of the mercury absorption test indicated that the smaller the grain size and the higher the concentration of TEOS, the percentage of mercury uptake would increase. In addition, the absorption percentage increased with the duration of absorption time given.

Composite Magnetic Sorbents Based on Iron Oxides in Different Polymer Matrices: Comparison and Application for Removal of Strontium

Introduction of magnetic nanoparticles into composite sorbents based on polymer matrices has received great attention due to the possibility of using cheap iron oxides and removing spent sorbents by means of magnetic separation. In the present paper, we discuss the problem of creating magnetic sorbents using two types of matrices as host materials: synthetic cation exchange resin and natural aminopolysaccharide chitosan. The possibilities of applying matrices for the in situ formation of oxide phases of a specified composition with the required content of an inorganic component in a composite material were estimated. The composition of the oxide phase formed in the composite material was studied, and particle sizes were evaluated by the method of X-ray diffraction analysis. Magnetic characteristics were investigated. Sorption characteristics with respect to strontium for the composites containing iron oxides were determined.