Determination of Ocular Irritancy Potential of Ophthalmic products using HET-CAM Method

Objective: The research is about the stability of ophthalmic products as they start to produce irritancy effects during their shelf life. The aim of the present study is to assess the stability of certain ophthalmic products by the HET-CAM (Hen’s Egg Test-Chorioallatonic membrane) Method. Method: Incubated Eggs were utilized for performing the HET-CAM assay according to the protocol prescribed by ICCVAM (The Inter agency Coordinating Committee on the Validation of Alternative Methods). Results: Calculation of various irritancy factors like hemorrhage time, vascular lysis time and coagulation time and to calculate HET-CAM score. Statistical interpretation of the data obtained by one-way ANOVA. Conclusion: Results have shown that the few ophthalmic products are slight and moderate irritants. None of the products are strong irritants. However further studies to be conducted to confirm the potential and safety of the products.

A systematic examination of the mucous membrane of the mouth (oral mucous membrane) – method of early diagnosis of precancerous diseases

The article presents information on the liability of the dentist for reducing the risk of development of pathological processes, including malignant neoplasms of the oral cavity. A thorough inspection of the oral mucous membrane, in accordance with WHO recommendations provides a systematic survey of all departments of the oral cavity and surrounding tissues. This tactic can significantly reduce the risk of development of pathological lesions of the oral cavity, to prevent malignant transformation or provide early diagnosis.

Microbiological and Physicochemical Research of Mountain Spring Waters in Vasiliovska Mountain, Municipality of Teteven, Bulgaria

Defined are the physicochemical properties of three mountain spring waters in the area of Vasiliovska mountain, Municipality of Teteven, Bulgaria. Vasiliovska mountain is in the central part of Stara Planina (Balkan) mountain. The methodology is including research of physicochemical parameters and microbiological indicators. It is needed the whole parameters to corresponds to Ordinance № 9 / 2001, Official State Gazette, issue 30, and decree № 178 / 23.07.2004 about the quality of water, intended for drinking purposes in Bulgaria, European Union [1,2,3]. The spring waters from the given three water sources are characterized by microbiological indicators, and the pathogenic micro-organisms in the samples from the springs water sources mentioned above are determined by the membrane method [4,5]. It is shown that according to 18 controlled parameters included in the study, the following springs in Vasiliovska mountain - “Babintsi” with water temperature 12.3°C, “Gechovoto” with water temperature 13.5°C, “Ignatov izvor” with water temperature 14.1°C, correspond to all controlled parameters according to Ordinance № 9 / 2001, Official State Gazette, issue 30, and decree № 178 / 23.07.2004 about the quality of water, intended for drinking purposes.