Nutritional Evaluation of Crambe Meal as a Partial Replacement of Soybean Meal in Nile Tilapia Diets

This study evaluated the apparent digestibility coefficient (ADC) of crambe meal (CM) and its potential to partially replace soybean meal (SM) protein in Nile tilapia diets. The ADC for dry matter, crude protein, ether extract, energy, amino acids, calcium and phosphorus of CM were assessed in fish (n=80; 65.30 ± 5.32 g). Subsequently, an 80-day feeding trial was conducted with Nile tilapia (n=140; 6.04 ± 0.25 g) randomly distributed in 20 experimental cages (70 L; seven fish cage-1) allocated in five circular tanks (1000 L) in recirculation water system, to evaluate the effects of replacement of SM by CM (0, 6, 12, 18 and 24% in isonitrogenous and isoenergetic diets) on growth, blood parameters, fillet yield and proximal composition. The CM shows good digestibility of protein (0.824) and amino acids (0.844) by Nile tilapia and its inclusion in the diet does not affect carcass and fillet yield or proximal composition. Fish fed diets with 24.0% of the SM replaced by CM showed the worst weight gain and feed conversion rate. The protein efficiency ratio decreased in fish fed diets with 12.0, 18.0 and 24.0% of the SM replaced by CM. Hemoglobin, mean corpuscular hemoglobin concentration, total plasma protein, glucose and alanine aminotransferase enzyme activity trend to increase at highest levels of CM in the diet. In conclusion, CM has potential to replace SM in Nile tilapia diets, due to high digestibility of protein and amino acids. However, anti-nutritional factors present in untreated CM interfere on the growth and nutrient utilization of Nile tilapia.

BLOOD AND ACID-BASE CHANGES IN CAILVES EICPERIMENTALLY INFECTED WITH SALMONELLA TYPHIMPRIUM

The experimental design of this study was included (12) Friesian calves aged between 3-5 weeks. Calves were divided into two groups. Group I consisted of (8) calves were infected experimentally with 1.5 x 10“ of Salmonella typhimurium and often that treatment with chloramphenicol and electrolytes fluid were takes place. Group II consisted of (4) calves were infected with organisms but without treatment. The clinical findings of the disease were characterized by two forms which includes the septicemic and enteric forms. Haematological changes revealed a variable number of the total and differential leukocytic count, increased PCV, fibnnogen and decreased of the total plasma protein. . The changes in the acid-based balance indicate the development of metabolic acidosis.

Hematological parameters and antibody titers to new castle diseases and avian influenza on extensive and semi-intensive system

The study evaluated the hematological parameters and antibody titers against Newcastle Disease (ND) and Avian Influenza (AI) on native chickens under extensive and semi-intensive rearing in Central Java, Indonesia. The target of the study was native chickens in Banyumas and Kebumen areas. The chickens were reared extensive and semi-intensively. The survey method was carried out by drawing 324 chicken blood samples for hematological tests and measuring antibody titer to ND and AI. The data obtained were analyzed using a general linear model (GLM) with the Systat ver.13 program. The results showed that native chicken in extensive and semi-intensive systems had different physiological statuses (P<0.05). The number of erythrocytes, hemoglobin levels, hematocrit values, total plasma protein levels, and heterophil-lymphocyte ratios was higher on semi-intensive than extensive system. However, the number of leucocytes was relatively high the same (P>0.05). In extensive system rearing, the number of native chickens with effective antibody titers against ND and AI was higher than the semi-intensive. The study concluded that native chickens reared in semi-intensive systems had a healthier physiological status than chickens in extensive systems. However, the effective antibody titers against ND and AI in the extensive and semi-intensive systems were still very low.

The replacement of fish meal with fermented Moringa leaves meal and its effect on the immune response of red tilapia (Oreochromis sp.)

Moringa (Moringa oleifera) has frequently been studied as an alternative and promising protein source in fish feed. Moringa leaves is among the potential and affordable plant-based protein source ingredient, as it protein, vitamin, beta-carotene, and various kind of minerals. Flavonoid, saponin, vitamin and alkaloids reportedly exhibit immune-stimulating activity which increase immune response. This study aims to evaluate the replacement of fish meal with fermented Moringa leaves meal in feed on the immune response of red tilapia (Oreochromis sp.). The research was conducted experimentally using a completely randomized design by four treatments in triplicate. The treatments include replacement of the fish meal with fermented moringa leaves meal at 0, 10, 20, and 30 %. Red tilapia with total length of 9 to 10 cm was fed twice daily for two months at a feeding rate of 3 % of total biomass. The results showed that replacement fish meal with fermented Moringa leaves meal as much as 20 % increases the percentage values of hematocrit and leukocrit, stimulates phagocytic activity and phagocytic index, suppresses monocyte and increases lymphocytes, and increases total plasma protein.

PSIX-13 Activation of cell signaling pathways by galectins in cow blood

Galectins are part of a conserved family of β-galactoside-binding proteins that contribute to critical biological events during mammalian gestation and increasingly recognized for a possible role in the immune response of the cow. The objective of this study was to evaluate the effect of Galectins on signal transduction and cell activation in cow blood. Whole blood was collected aseptically from the jugular vein of healthy Holstein Friesian dairy cows (N=3). Blood samples (2.5ml) in duplicate were treated with 150µl of the four different type of recombinant galectins (1, 3, 4, and 9) respectively and untreated samples were served as control. The concentration of total plasma protein was determined using the Pierce BCA kit. Protein expression profiling was performed using1,358 antibodies on the Full Moon BioSystems’ Signaling Explorer antibody array covering 20 cell signaling pathways, as recommended by the manufacturer using an Agilent microarray scanner. Data normalization was performed using GeneSpring GX software to generate fold changes in gene expression and then filtered to obtain a list of significantly upregulated and downregulated genes. Features were extracted from protein array images of samples treated with Galectin 1, 3, 4, 9, and untreated sample as a control group. Treatment with all four Galectins increased the concentration of total plasma protein. Average increases due to treatment with Gal1, Gal3, Gal4, and Gal9 were 27%, 10%, 20%, and 14% respectively. ANOVA test showed significance difference among the groups (p &lt; 0.05). Dunnett option was used to compare each of the treated samples to the control group as a baseline. The results also showed that there was significant difference between the control group and any of the treated group (p &lt; 0.05). Distinct signaling pathways are activated in response to Galectin exposure. Further studies are needed to define their regulation and functional impact on cow health.

Non-specific immune response of Pacific white shrimp Litopenaeus vannamei by supplementation of sodium alginate of Sargassum collected from Lampung Indonesia

This study aims to determine the effectiveness of supplementation of sodium (Na) alginate Sargassum sp. from Lampung waters to enhance the non-specific immune response and the resistence of Pacific white shrimp (Litopenaeus vannamei) against white spot disease. This study used a completely randomized design with 3 treatments, namely feeding without alginate supplementation (A) or control, dietary Na alginate Sargassum supplementation at a dose of 2.0 (B), and 4.0 g kg−1 feed (C), each with four replications, for 14 days. Hemolymph sampling was performed on days 0, 7 and 14 to observe the parameter of shrimp hematology included total haemocyte count (THC), phagocytocyte activity (PA), phagocytic index (PI), and total plasma protein (TPP). The hepatopancreas histology profile and water quality were observed at the end of treatment. Data were analyzed by Anova at 95% of confidence interval and continued with Duncan test. Result showedthat the supplementation of Na alginat from Sargassum sp. significantly enhance several shrimp immune response namely THC, PA, and TPP. Meanwhile, the histology of the hepatopancreas of both shrimp treatment and control showed not significantly effect on tissue damage. The water quality was still in normal condition during the treatment. The results of this study indicate that the application of Sargassum alginate supplementation at a dose of 2gr/kg of feed is the best treatment to enhance the immune response of Pacific white shrimp.

Effect of Physiological Status And Parity On Metabolic And Trace Elements Profile of Crossbred Rambouillet Sheep of Himalayan Region

The study was designed to evaluate the effect of physiological status and parity on metabolic profile in crossbred Rambouillet ewes of Himalyan region. The study was conducted on 20 ewes divided into two groups, primiparous (PP) and multiparous (MP) with 10 ewes in each group. Blood samples were collected on 4- and 1-week pre-lambing and 1- and 4-weeks post-lambing to measure metabolic parameters and minerals. The glucose(p<0.01), Total plasma protein (TPP) (p<0.05), albumin (p<0.05), BUN (p<0.05), cholesterol (p<0.05), triglyceride (p<0.01), HDL-C (P<0.05), Calcium (p<0.01), Phosphours (p<0.05), Magnesium (p<0.01), Cu (p<0.05), and Zn (p<0.01) levels revealed significant change along the time with the concentration decreasing 3 weeks pre lambing to immediate post lambing thereafter levels increased steadily. Significant increase (P<0.01) was observed in NEFA, AST, GGT, Iron(P<0.05), and bilirubin (P<0.05) concentrations along the sampling time. No group difference was observed in any of the parameters however, parity and time interaction was observed in Glucose, NEFA, GGT, calcium, and phosphorus. While NEFA levels were significantly high in pre-lambing in PP ewes compared to MP ewes, the post-lambing levels were significantly high in MP ewes. Pre-lambing levels of GGT were at par between the two groups; however, post-lambing levels were significantly high in MP ewes. Glucose, Ca and Pi were low during pre-lambing in PP ewes and post-lambing in MP ewes. The result showed that ewes shows a significant change in metabolic profile and trace minerals during late gestation and immediate post-partum, however these changes were more pronounced during late gestation in primiparous and post lambing in multiparous.

Dynamic change of blood profile in rat models with acute skin injury artificially infected with methicillin-resistant Staphylococcus aureus

Background and Aim: A wound is a common problem for humans and animals. The wound becomes more severe if it is infected by bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA). The wound healing mechanism involves various factors, either in the local tissue or the bloodstream. However, the presentation of infected wound healing regarding its impacts on the dynamic change of blood profile is not clearly understood. This study aimed to explore the impacts of wound creation on the blood profile in rat models with and without being artificially infected by MRSA. Materials and Methods: Thirty male Sprague-Dawley rats (6 months old; weight, 300 g) were used as the model. They were divided into three groups: Without wound creation (C), wounded without infection (CW), and wounded and artificially infected by MRSA (CWI). Groups CW and CWI were shaved and induced with 4 mm two-round full-thickness biopsy on the back. Furthermore, group CWI was artificially infected by 105 colony-forming units of MRSA. The blood samples were collected through the tail vein from days 1 to 5. The blood parameters included blood profile, total plasma protein, C-reactive protein, CD4+, CD8+, CD4+/CD8+, and COX-2. The data were analyzed using the Statistical Package for the Social Sciences, version 16 (SPSS, IBM, Armonk, NY, USA). Results: The result showed that the presentation of a wound with and without MRSA infection significantly changed the total erythrocytes, leukocytes, neutrophils, lymphocytes, total plasma protein, C-reactive protein, and the subset of circulatory CD4+, CD8+, and COX-2 (p≤0.05). In addition, the wound infected with MRSA impacts the mean corpuscular volume (p≤0.05). Conclusion: Moreover, the presentation of the wound with and without MRSA infection induces dynamic changes on various blood profile parameters.

Effect of Vibrotherapy on Body Fatness, Blood Parameters and Fibrinogen Concentration in Elderly Men

Elderly people need activities that will positively contribute to a satisfactory process of getting older. Vibration training uses mechanical stimulus of a vibrational character that, similarly to other forms of physical activity, affects metabolic processes and conditions of health. The aim of this work was to assess the influence of thirty vibration treatments on body fatness, hematologic and rheologic indexes of blood, and proteinogram and fibrinogen concentration in elderly men’s blood. The study included twenty-one males, aged 60–70 years (mean age 65.3 ± 2.7), who were randomly assigned into a vibrotherapy group (VG) and took part in interventions on mattresses generating oscillatory-cycloid vibrations, and a control group (CG), without interventions. In all patients the following assessments were performed twice: an assessment of body fatness using the bioimpedance method, a complete blood count with a hematology analyzer, and erythrocyte aggregation by a laser-optical rotational cell analyzer; whereas, total plasma protein and fibrinogen values were established, respectively, by biuret and spectrophotometric methods. In order to compare the impact of vibrotherapy on changes in the analyzed variables, analysis of variance (ANOVA) or the Wilcoxon test were used. After applying thirty vibration treatments in the VG, a significant decrease in body fatness parameters was confirmed: BM (∆BM: −2.7 ± 2.0; p = 0.002), BMI (∆BMI: −0.9 ± 0.7; p = 0.002), BF (∆BF: −2.5 ± 2.5; p = 0.013), and %BF (∆%BF: −2.0 ± 2.7; p = 0.041), as well as in RBC (∆RBC: −0.1 ± 0.1; p = 0.035). However, changes in erythrocyte aggregation and proteinogram were not confirmed. It was found that after thirty treatments with VG, a significant decrease of fibrinogen level took place (∆ = −0.3 ± 0.3, p = 0.005). Application of thirty vibrotherapy treatments positively affected body fatness parameters and fibrinogen concentrations in the examined. However, further research should include a greater number of participants.

Estimation of the selectivity of sorbents for IgG binding based on tripeptide ligands

Biospecific sorbents for the removal of IgG and subclasses from biological fluids based on oligopeptides, containing aromatic amino acid residues, were created. The selectivity properties of specific sorbents for IgM, IgE, and plasma proteins were evaluated. It was found that the created sorbents exhibit the low activity to the total plasma protein, albumin, IgM, IgE and are highly specific for IgG.