Bleach correction ImageJ plugin for compensating the photobleaching of time-lapse sequences

During the capturing of the time-lapse sequence of fluorescently labeled samples, fluorescence intensity exhibits decays. This phenomenon is known as 'photobleaching' and is a widely known problem in imaging in life sciences. The photobleaching can be attenuated by tuning the imaging set-up, but when such adjustments only partially work, the image sequence can be corrected for the loss of intensity in order to precisely segment the target structure or to quantify true intensity dynamics. We implemented an ImageJ plugin that allows the user to compensate for the photobleaching to estimate the non-bleaching condition with choice of three different algorithms: simple ratio, exponential fitting, and histogram matching methods. The histogram matching method is a novel algorithm for photobleaching correction. This article presents details and characteristics of each algorithm based on application to actual image sequences.

Scouring and Bleaching on One Bath for Tussah Silk

In this paper the one bath process of scouring and bleaching was applied to degum and bleach tussah silk. In this process degumming and bleaching of tussah silk was achieved only through one step. The effects of salt, decolorizer, treat time and temperature on whiteness, degumming rate and break strength were investigated. The optimal degumming and bleaching condition was that the concentration of Na2SiO3salt was 10 o.w.f. %, concentration of hydrogen peroxide was 10 o.w.f %, the scouring and bleaching time was 40 min and temperature was 95 °C. Under the optimal scouring and bleaching condition the whiteness of tussah silk was 82, degumming rate was 11.5% and the breaking strength was 370 N.

Dyeing and Finishing of the Wool Regeneration Protein and Polyacrylonitrile Copolymerization Fiber

Partial dyeing and finishing properties of the copolymerization fiber of wool regeneration protein and polyacrylonitrile are analyzed in this article. The optimum bleaching condition is 30%H2O230g/L, Na2SiO36%(o.w.f) for 60min at 60°C.This new fiber can be dyed by acid dye and cationic dye in one-bath one-step dyeing process. Interaction between anionic surfactant NNO and cationic dye has been investigated using conductometric examinations. The results are that the mass ratio of cationic dye and NNO is 1:7-9, the ratio of anionic dye and cationic dye is 2:8 which is the same as the constituents of fiber. The method of making this special dyestuff is drying the cationic dye (liquor) and NNO (liquor) compound, and then mixed with acid dye, thus trichromatic dyestuffs have been made on the whole through experiment.

TCF Short-Sequence Bleaching of Pinus Kesiya Pulp Made by Low-Temperature Kraft Pulping

In this paper, the TCF short-sequence bleaching (OQPaP) of pinus kesiya pulp from low-temperature kraft pulping with kappa number of 21.32 and viscosity of 1194mL•g-1 (the maximum pulping temperature was only 150°C) was optimized. Under the optimum bleaching condition, the bleached pulp properties of low-temperature kraft pulp were as follows: yield 93.1%, brightness 85.31% ISO, kappa number 1.02, viscosity 751mL•g-1 and PC number 0.28. And physical properties of handsheet (after beating to 45oSR) were as follows: breaking length 5.41km, bursting index 3.48kPa•m2•g-1, tearing index 8.4mN•m2•g-1, and folding endurance 146 times.

Study on Magnesium Hydroxide-Based Hydrogen Peroxide Bleaching Process of Nigra poplar CTMP

The Mg(OH)2-based peroxide bleaching process of Nigra poplar CTMP pulp was confirmed. The optimal bleaching condition were as follows: 4% H2O2,1% Mg(OH)2, 15% pulp consistency, the reaction temperature was 80°C and the suitable reaction time was 2 hours. The Mg(OH)2-based bleaching process shows significant benefits over sodium hydroxide process. In contrast, magnesium hydroxide bleaching generates higher pulp yield and lower effluent COD at the same chemical dosage. The study shows peroxide bleaching of CTMP pulp with magnesium hydroxide as alkali source is to be an option for the high-yield pulp bleaching.