Understanding the Impact of Vertical Canopy Position on Leaf Spectra and Traits in an Evergreen Broadleaved Forest

Little attention has been paid to the impact of vertical canopy position on the leaf spectral properties of tall trees, and few studies have explored the ability of leaf spectra to characterize the variation of leaf traits across different canopy positions. Using a tower crane, we collected leaf samples from three canopy layers (lower, middle, and upper) and measured eight leaf traits (equivalent water thickness, specific leaf area, leaf carbon content, leaf nitrogen content, leaf phosphorus content, leaf chlorophyll content, flavonoid, and nitrogen balance index) in a subtropical evergreen broadleaved forest. We evaluated the variability of leaf traits and leaf spectral properties, as well as the ability of leaf spectra to track the variation of leaf traits among three canopy layers for six species within the entire reflectance spectrum. The results showed that the eight leaf traits that were moderately or highly correlated with each other showed significant differences along the vertical canopy profile. The three canopy layers of leaf spectra showed contrasting patterns for light-demanding (Castanopsis chinensis, Castanopsis fissa, Schima superba, and Machilus chinensis) and shade-tolerant species (Cryptocarya chinensis and Cryptocarya concinna) along the vertical canopy profile. The spectra at the lower and upper canopy layers were more sensitive than the middle layer for tracking the variation of leaf chlorophyll and flavonoid content. Our results revealed that it is important to choose an appropriate canopy layer for the field sampling of tall trees, and we suggest that flavonoid is an important leaf trait that can be used for mapping and monitoring plant growth with hyperspectral remote sensing.

Predicting Equivalent Water Thickness in Wheat Using UAV Mounted Multispectral Sensor through Deep Learning Techniques

The equivalent water thickness (EWT) is an important biophysical indicator of water status in crops. The effective monitoring of EWT in wheat under different nitrogen and water treatments is important for irrigation management in precision agriculture. This study aimed to investigate the performances of machine learning (ML) algorithms in retrieving wheat EWT. For this purpose, a rain shelter experiment (Exp. 1) with four irrigation quantities (0, 120, 240, 360 mm) and two nitrogen levels (75 and 255 kg N/ha), and field experiments (Exps. 2–3) with the same irrigation and rainfall water levels (360 mm) but different nitrogen levels (varying from 75 to 255 kg N/ha) were conducted in the North China Plain. The canopy reflectance was measured for all plots at 30 m using an unmanned aerial vehicle (UAV)-mounted multispectral camera. Destructive sampling was conducted immediately after the UAV flights to measure total fresh and dry weight. Deep Neural Network (DNN) is a special type of neural network, which has shown performance in regression analysis is compared with other machine learning (ML) models. A feature selection (FS) algorithm named the decision tree (DT) was used as the automatic relevance determination method to obtain the relative relevance of 5 out of 67 vegetation indices (Vis), which were used for estimating EWT. The selected VIs were used to estimate EWT using multiple linear regression (MLR), deep neural network multilayer perceptron (DNN-MLP), artificial neural networks multilayer perceptron (ANN-MLP), boosted tree regression (BRT), and support vector machines (SVMs). The results show that the DNN-MLP with R2 = 0.934, NSE = 0.933, RMSE = 0.028 g/cm2, and MAE of 0.017 g/cm2 outperformed other ML algorithms (ANN-MPL, BRT, and SVM- Polynomial) owing to its high capacity for estimating EWT as compared to other ML methods. Our findings support the conclusion that ML can potentially be applied in combination with VIs for retrieving EWT. Despite the complexity of the ML models, the EWT map should help farmers by improving the real-time irrigation efficiency of wheat by quantifying field water content and addressing variability.

A Review of Crop Water Stress Assessment Using Remote Sensing

Currently, the world is facing high competition and market risks in improving yield, crop illness, and crop water stress. This could potentially be addressed by technological advancements in the form of precision systems, improvements in production, and through ensuring the sustainability of development. In this context, remote-sensing systems are fully equipped to address the complex and technical assessment of crop production, security, and crop water stress in an easy and efficient way. They provide simple and timely solutions for a diverse set of ecological zones. This critical review highlights novel methods for evaluating crop water stress and its correlation with certain measurable parameters, investigated using remote-sensing systems. Through an examination of previous literature, technologies, and data, we review the application of remote-sensing systems in the analysis of crop water stress. Initially, the study presents the relationship of relative water content (RWC) with equivalent water thickness (EWT) and soil moisture crop water stress. Evapotranspiration and sun-induced chlorophyll fluorescence are then analyzed in relation to crop water stress using remote sensing. Finally, the study presents various remote-sensing technologies used to detect crop water stress, including optical sensing systems, thermometric sensing systems, land-surface temperature-sensing systems, multispectral (spaceborne and airborne) sensing systems, hyperspectral sensing systems, and the LiDAR sensing system. The study also presents the future prospects of remote-sensing systems in analyzing crop water stress and how they could be further improved.

Close-range Hyperspectral Spectroscopy Reveals Leaf Water Content Dynamics

Water plays a crucial role in maintaining plant functionality and drives many ecophysiological processes. The distribution of water resources is in a continuous change due to global warming affecting the productivity of ecosystems around the globe, but there is a lack of non-destructive methods capable of continuous monitoring of plant and leaf water content that would help us in understanding the consequences of the redistribution of water. We studied the utilization of novel small hyperspectral sensors in the 1350-2450 nm spectral range in non-destructive estimation of leaf water content in laboratory and field conditions. We found that the sensors captured up to 96% of the variation in equivalent water thickness (EWT, g/m2) and up to 90% of the variation in relative water content (RWC). These laboratory findings were supported by field measurements, where repeated leaf spectra measurements were in good agreement (R2=0.79) with a time-lagged change of tree xylem diameter. Further tests were done with an indoor plant (Dracaena marginate Lem.) by continuously measuring leaf spectra while drought conditions developed, which revealed detailed diurnal dynamics of leaf water content. We conclude that close-range hyperspectral spectroscopy can provide a novel tool for continuous measurement of leaf water content at the single leaf level and help us to better understand plant responses to varying environmental conditions.

Impact of Modeling Abstractions When Estimating Leaf Mass per Area and Equivalent Water Thickness Over Sparse Forests Using a Hybrid Method

Equivalent water thickness (EWT) and leaf mass per area (LMA) are important indicators of plant processes, such as photosynthetic and potential growth rates and health status, and are also important variables for fire risk assessment. Retrieving these traits through remote sensing is challenging and often requires calibration with in situ measurements to provide acceptable results. However, calibration data cannot be expected to be available at the operational level when estimating EWT and LMA over large regions. In this study, we assessed the ability of a hybrid retrieval method, consisting of training a random forest regressor (RFR) over the outputs of the discrete anisotropic radiative transfer (DART) model, to yield accurate EWT and LMA estimates depending on the scene modeling within DART and the spectral interval considered. We show that canopy abstractions mostly affect crown reflectance over the 0.75–1.3 μm range. It was observed that excluding these wavelengths when training the RFR resulted in the abstraction level having no effect on the subsequent LMA estimates (RMSE of 0.0019 g/cm2 for both the detailed and abstract models), and EWT estimates were not affected by the level of abstraction. Over AVIRIS-Next Generation images, we showed that the hybrid method trained with a simplified scene obtained accuracies (RMSE of 0.0029 and 0.0028 g/cm2 for LMA and EWT) consistent with what had been obtained from the test dataset of the calibration phase (RMSE of 0.0031 and 0.0032 g/cm2 for LMA and EWT), and the result yielded spatially coherent maps. The results demonstrate that, provided an appropriate spectral domain is used, the uncertainties inherent to the abstract modeling of tree crowns within an RTM do not significantly affect EWT and LMA accuracy estimates when tree crowns can be identified in the images.

The influence of vegetation water dynamics on the ASCAT backscatter-incidence angle relationship in the Amazon

Microwave observations are sensitive to plant water content and could therefore provide essential information on biomass and plant water status in ecological and agricultural applications. The combined data record of the C-band scatterometers on ERS 1/2, the Metop series and the planned Metop Second Generation satellites will span over 40 years, which would provide a long-term perspective on the role of vegetation in the climate system. Recent research has indicated that the unique viewing geometry of ASCAT could be exploited to observe vegetation water dynamics. The incidence angle dependence of backscatter can be described with a second order polynomial, the slope and curvature of which are related to vegetation. In a study limited to grasslands, seasonal cycles, spatial patterns and interannual variability in the slope and curvature were found to vary among grassland types and were attributed to differences in moisture availability, growing season length and phenological changes. To exploit ASCAT slope and curvature for global vegetation monitoring, their dynamics over a wider range of vegetation types needs to be quantified and explained in terms of vegetation water dynamics. Here, we compare ASCAT data with meteorological data and GRACE Equivalent Water Thickness (EWT) to explain the dynamics of ASCAT backscatter, slope and curvature in terms of moisture availability and demand. We consider differences in the seasonal cycle, diurnal differences, and the response to the 2010 and 2015 droughts across ecoregions in the Amazon basin and surroundings. Results show that spatial and temporal patterns in backscatter reflect moisture availability indicated by GRACE EWT. Slope and curvature dynamics vary considerably among the ecoregions. The evergreen forests, often used as a calibration target, exhibit very stable behaviour even under drought conditions. The limited seasonal variation follows changes in the radiation cycle, and may indicate phenological changes such as litterfall. In contrast, the diversity of land cover types within the Cerrado region results in considerable heterogeneity in terms of the seasonal cycle and the influence of drought on both slope and curvature. Seasonal flooding in forest and savanna areas also produced a distinctive signature in terms of the backscatter as a function of incidence angle. This improved understanding of the incidence angle behaviour of backscatter increases our ability to interpret and make optimal use of the ASCAT data record and VOD products for vegetation monitoring.

AN INVERSION APPROACH FOR BIOCHEMICAL PARAMETERS OF VEGETATION BASED ON THE PROSPECT-5 MODEL

Accurate inversion of vegetation biochemicals using the PROSPECT model mostly depends on a proper inversion approach, including a suitable optimizing algorithm, appropriate dependent variables, and different properties from spectra of reflectance (R) and transmittance (T). In this paper, we propose a special inversion method using PROSPECT-5 and then explore its effectiveness in inverting chlorophyll, carotenoids, equivalent water thickness, and dry matter per area data from the ANGERS database. The inversion strategy includes (i) an optimal algorithm with constrained bounds (fminsearchbnd) to replace the common function fminsearch, (ii) and four parameters are considered together and separately as dependent variables of models, (iii) Using properties from the spectra of R, T and combined R&T to invert the above four biochemical parameters. The results show that fminsearchbnd can improve the model's R2 based on a field-measured database. Moreover, using the entire set of parameters together as the model inputs is more effective than using single parameters separately. T spectra are favoured for all parameter inversions in the model database while being inapplicable in the ANGERS database. These findings provide an appropriate inversion strategy for the PROSPECT-5 model in vegetation biochemical parameters analysis and suggest further research to develop an accurate inversion process for vegetation based on various physical models.

Retrieving Photometric Properties and Soil Moisture Content of Tidal Flats Using Bidirectional Spectral Reflectance

Moisture content in tidal flats changes frequently and spatially on account of tidal fluctuations, which greatly influence the reflectance of the tidal flat surface. Precise prediction of the spatial-temporal variation of tidal flats’ moisture content is an important foundation of surface bio-geophysical information research by remote sensing. In this paper, we first measured the multi-angle reflectance of soil samples obtained from tidal flats in the northeastern Dongtai, Jiangsu Province, China, in the laboratory. Then, based on the particle swarm optimization (PSO) algorithm, we retrieved the photometric characteristics of the soil surface by employing the SOILSPECT bidirectional reflectance model. Finally, the soil moisture content was retrieved by introducing the equivalent water thickness of the soil. The results showed that: (i) A significant correlation existed between the retrieved equivalent water thickness and the measured soil moisture content. The SOILSPECT model is capable of estimating soil moisture with high precision by using multi-angle reflectance. (ii) Retrieved values of single scattering albedo (ω) were consistent with the variation of soil moisture content. The roughness parameter (h) and the asymmetry factor (Θ) were consistent with the structure and particle composition of the soil surface in dry soil samples. (iii) When the soil samples were soaked with water, the roughness parameter (h) and the type of scattering on the soil surface both showed irregular changes. These results support the importance of using the measured soil particle size as one of the parameters for the retrieval of soil moisture content, which is a method that should be used cautiously, especially in tidal flats.

Estimation of leaf water content from hyperspectral data of different plant species by using three new spectral absorption indices

The leaf equivalent water thickness (EWT, g cm−2) and fuel moisture content (FMC, %) are key variables in ecological and environmental monitoring. Although a variety of hyperspectral vegetation indices have been developed to estimate the leaf EWT and FMC, most of these indices are defined considered two or three specific bands for a specific plant species, which limits their applicability. In this study, we proposed three new spectral absorption indices (SAI970, SAI1200, and SAI1660) for various plant types by considering the symmetry of the spectral absorption at 970 nm, 1200 nm and 1660 nm and spectral heterogeneity of different leaves. The indices were calculated considering the absorption peak and shoulder bands of each leaf instead of the same specific bands for all leaves. A pooled dataset of three tree species (camphor (VX), capricorn (VJ), and red-leaf plum (VL)) was used to test the performance of the SAIs in terms of the leaf EWT and FMC estimation. The results indicated that, first, SAI1200 was more suitable for estimating the EWT than FMC, whereas SAI970 and SAI1660 were more suitable for estimating the FMC. Second, SAI1200 achieved the most accurate estimation of the EWT with a cross-validation coefficient of determination (Rcv2) of 0.845 and relative cross-validation root mean square error (rRMSEcv) of 8.90%. Third, SAI1660 outperformed the other indices in estimating the FMC at the leaf level, with an Rcv2 of 0.637 and rRMSEcv of 8.56%. Fourth, SAI970 achieved a moderate accuracy in estimating the EWT (Rcv2 of 0.25 and rRMSEcv of 19.68%) and FMC (Rcv2 of 0.275 and rRMSEcv of 12.10%) at the leaf level. These results can enrich the application of the SAIs and demonstrate the potential of using SAI1200 to determine the leaf EWT and SAI1660 to obtain the leaf FMC among various plant types.

Improving the Selection of Vegetation Index Characteristic Wavelengths by Using the PROSPECT Model for Leaf Water Content Estimation

Equivalent water thickness (EWT) is a major indicator for indirect monitoring of leaf water content in remote sensing. Many vegetation indices (VIs) have been proposed to estimate EWT based on passive or active reflectance spectra. However, the selection of the characteristics wavelengths of VIs is mainly based on statistical analysis for specific vegetation species. In this study, a characteristic wavelength selection algorithm based on the PROSPECT-5 model was proposed to obtain characteristic wavelengths of leaf biochemical parameters (leaf structure parameter (N), chlorophyll a + b content (Cab), carotenoid content (Car), EWT, and dry matter content (LMA)). The effect of combined characteristic wavelengths of EWT and different biochemical parameters on the accuracy of EWT estimation is discussed. Results demonstrate that the characteristic wavelengths of leaf structure parameter N exhibited the greatest influence on EWT estimation. Then, two optimal characteristics wavelengths (1089 and 1398 nm) are selected to build a new ratio VI (nRVI = R1089/R1398) for EWT estimation. Subsequently, the performance of the built nRVI and four optimal published VIs for EWT estimation are discussed by using two simulation datasets and three in situ datasets. Results demonstrated that the built nRVI exhibited better performance (R2 = 0.9284, 0.8938, 0.7766, and RMSE = 0.0013 cm, 0.0022 cm, 0.0030 cm for ANGERS, Leaf Optical Properties Experiment (LOPEX), and JR datasets, respectively.) than that the published VIs for EWT estimation. It is demonstrated that the built nRVI based on the characteristic wavelengths selected using the physical model exhibits desirable universality and stability in EWT estimation.