Pengembangan Set Multipleks Penanda DNA Mikrosatelit untuk Analisis Variasi Genetik Padi dan Kedelai

<p>Detection of multiplex microsatellite markers in a<br />single capillary array on a laser detection system is traditionally<br />conducted with specific primers that are labelled with<br />fluorescent dyes. An alternative method using fluorescent<br />labels that are appended to 5’ end of universal primer M13<br />instead of to the specific primers offers flexibility in<br />designning multiplex panels and a less expensive method.<br />Allele size range of microsatellite loci that can be grouped in<br />multiplex panels can be accurately estimated by pooling and<br />analyzing DNA samples from several genotypes simultaneously.<br />This paper describes the procedure in development<br />of microsatellite multiplex panels using M13 fluorescentlylabelled<br />and estimation of allele size range based on pooled<br />DNA strategies. Two multiplex panels of PCR amplification<br />products for rice consisting of 15 loci and three panels for<br />soybean consisting of 10 loci have been designed. The<br />panels have been applied to 50 accessions of rice and soybean<br />with fairly good results. Further characterization of<br />allele size range, however, is required prior to the application<br />of these panels to diverse genotypes. The procedure<br />described here should be applicable in the development of<br />multiplex panels of other species.</p>

Molecular characterization and morphological clustering of exotic early maturing rice (Oryza sativa L.) lines

Characterization and variability analysis is important for the improvement of crop plant. This study aimed to evaluate the morphological and molecular variation of exotic early maturing rice (Oryza sativa L.) lines. A total of 32 exotic rice lines collected from different locations were genotyped and clustered using selected SSR markers. Based on morphological dendrogram, the lines were grouped into three clusters viz.I, II and III. Cluster I, cluster II and cluster III had 12, 11, 9 lines respectively. The results showed that the varieties were closely related belonging to the same cluster. DNA Markers namely Simple Sequence Repeats (SSR) is a useful tool for assessing genetic variations and resolving cultivar identities. Positive correlations were found between gene diversity, number of allele, the allele size range and the maximum number of repeats. Among the primers used RM147 identified more number of alleles and average PIC was 0.88. The UPGMA dendrogram based on Nei’s (1972) genetic distance grouped the 32 rice lines into three major clusters. This result indicates that the line which formed grouped together, they are less diverse. A significant level of polymorphism based on morphological and molecular levels was observed. Being grouped into three clusters C1-4-11-7P-2P-1P and IR 79201-49-1-1-1 could be utilized as potential parents for the improvement of yield in early maturing rice lines. DOI: http://dx.doi.org/10.3329/jbau.v11i2.19900 J. Bangladesh Agril. Univ. 11(2): 233-240, 2013

Genetic Diversity and Gene Flow in Yellowstone Basin Pronghorn (Antilocapra Americana)

Yellowstone National Park's northern range is home to a geographically isolated population of pronghorn (Antilocapra americana) greatly reduced from historic levels of abundance. The genetic consequences of demographic declines in this population over the past 150 years are unknown, and the rates of genetic exchange among this and other populations in the Yellowstone basin are also unknown. We used 18 nuclear DNA microsatellite loci and noninvasively collected fecal samples from 73 individuals to quantify basic population genetic parameters and gene flow within and among the Yellowstone pronghorn population, a new population in the Paradise Valley, and a large population near Livingston, MT. Evidence for a genetic bottleneck in Yellowstone was strong based on several tests of heterozygote excess (Wilcoxon tests P≤ 0.033) and the relative ratio of allele richness to allele size range (M =0.283). However, mean population heterozygosity was high (HO = 0.665), and no indications of inbreeding were detected. Gene flow from elsewhere in the Yellowstone basin to Yellowstone National Park is extremely low. However, emigration from Yellowstone to the Paradise Valley population is high (26.7%), reinforcing conclusions based on behavioral data that this new population was founded by Yellowstone individuals. Despite years of population decline and genetic isolation, Yellowstone pronghorn appear to retain substantial genetic diversity.