UJI ATRAKTAN PADA EKSTRAK PURUN TIKUS (Eleocharis dulcis) SEBAGAI PERANGKAP HAMA PENGGEREK BATANG PADI PUTIH

Purun tikus (Eleocharis dulcis) are wild plants found on swamp land which are quite abundant in South Kalimantan. This research was conducted to find out the most active extract from Chinese water chestnut as an attractant of white rice stem borer (Scirpophaga innotata). Purun tikus were made into extracts using the stepwise extraction method using n-hexane, ethyl acetate, and methanol, with yields of 0.74%, 0.81%, and 7.58%, respectively. The results of the attractant test on purun tikus extract showed that the extract of n-hexane purun tikus was the most active extract as an attractant in white rice stem borer (Scirpophaga innotata). The results of the analysis using GC-MS showed that the extract of n-hexane purun tikus contained several compounds, namely 2-methyltetrahydrofuran, isopulegol, citronellal, citronellyl acetate, βcitronellol, Z-citral, 1-bromo-3,7-dimethyl-2,6-octadiene, citral, linalyl acetate, trans-caryophyllen, trans-phytol, verticellol.

Biomass of a Psychrophilic Fungus as a Biocatalyst for Efficient Direct Esterification of Citronellol

A biomass-bound lipase from psychrophilic Chrysosporium pannorum A-1 is an efficient biocatalyst for direct esterification of β-citronellol and acetic acid in an organic solvent. The biomass is effectively produced by fungal submerged culture at 20 ℃, which results in lower energy consumption during the production of biocatalyst. Supplementation of the culture medium with calcium carbonate together with olive oil contributed to a significant increase in the active biomass of mycelium in one batch culture and increased the efficiency of the biocatalyst. Biomass-bound lipase showed high catalytic activity in a broad temperature range of 30–60 °C and stability up to 70 °C. A maximum molar conversion value of 98% was obtained at 30 °C in n-hexane using a 2:1 alcohol-to-acid molar ratio and 3% w/v of the biocatalyst within 24 h. The high equimolar concentration of the substrates (200 mM) did not have an adverse effect on mycelial biomass activity. Dry mycelium of C. pannorum is a promising biocatalyst for large-scale biosynthesis of citronellyl acetate, given its low-cost production, high activity at low temperatures, and reusability in a minimum of seven 24-h biocatalytic cycles.

Identification of Floral Scent Profiles in Bearded Irises

Bearded irises are ornamental plants with distinctive floral fragrance grown worldwide. To identify the floral scent profiles, twenty-seven accessions derived from three bearded iris, including Iris. germanica, I. pumila and I. pallida were used to investigate the composition and relative contents of floral scent components by headspace solid-phase microextraction (HS-SPME) and gas chromatography–mass spectrometry (GC-MS). A total of 219 floral scent components were detected in blooming flowers. The scent profile varied significantly among and within the three investigated species. Principal component analysis (PCA) indicated that terpenes, alcohols and esters contributed the most to the floral scent components and 1-caryophyllene, linalool, citronellol, methyl cinnamate, β-cedrene, thujopsene, methyl myristate, linalyl acetate, isosafrole, nerol, geraniol were identified as the major components. In a hierarchical cluster analysis, twenty-seven accessions could be clustered into six different groups, most of which had representative scent components such as linalool, citronellyl acetate, thujopsene, citronellol, methyl cinnamate and 1-caryophyllene. Our findings provide a theoretical reference for floral scent evaluation and breeding of bearded irises.

Composition variation of essential oil of Cymbopogon spp. growing in Garhwal region of Uttarakhand, India

The present study was aimed to find out the chemical constituents of essential oils of cymbopogon species,viz. Cymbopogon martinii var motia, Cymbopogon flexuosus Nees., Cymbopogon winterianus Jowitt., growing in Garhwal region of Uttarakhand, India. The essential oils were obtained by hydro-distillationand subjected to detailed Gas Chromatography-Mass Spectroscopy (GC-MS) analysis in order to determine the variation in their volatile constituents. Twenty seven compounds are α-pinene, β-myrcene, Limonene, Cis-b-Ocimene, pCymene ,Terpinolene,6-Methyl hept-5-en-2-one, Citronellal, Linalool, Linalyl acetate, β-Elemene, β-Caryophyllene, Citronellyl acetate, Neral, α– Terpineol, Borneol,Gerainal, γ-Cadinene,Geranyl acetate, Citronellol,Nerol,Geraniol, Caryophyllene oxide,Germacrene—D-4-ol,Elemol, Epi-α-cadinol, δ-Cadinol representing 92.24 to 95.86% of the oil compositions as identified. While comparing the common constituents of three different species of cymbopogon taxa , the remarkable variation in compositions of essential oil was observed and it was in concentration of nearl (0.40;34.9;1.6), gerainal (nil;47.5;0.96) geraniol (82.5;4.5;20.15) , citronellol (T;0.2;12.39) , citronellal (0.1;0.5;36.19) , and linalool (0.8;1.6;0.96) for C.martini, C.flexuosus, C. winterianus respectively

A New Source of Elemol Rich Essential Oil and Existence of Multicellular Oil Glands in Leaves of theDioscoreaSpecies

Dioscoreaspecies is a very important food and drug plant. The tubers of the plant are extensively used in food and drug purposes owing to the presence of steroidal constituent’s diosgenin in the tubers. In the present study, we report for the first time that the leaves ofDioscorea compositaandDioscorea floribundagrown under the field conditions exhibited the presence of multicellular oil glands on the epidermal layers of the plants using stereomicroscopy (SM) and scanning electron microscopy (SEM). Essential oil was also isolated from the otherwise not useful herbage of the plant, and gas chromatographic-mass spectroscopy analysis revealed confirmation of the essential oil constituents. Out of the 76 compounds detected inD. floribundaand 37 fromD. compositaessential oil, major terpenoids which are detected and reported forDioscorealeaf essential oil areα-terpinene, nerolidol, citronellyl acetate, farnesol, elemol,α-farnesene, valerenyl acetate, and so forth. Elemol was detected as the major constituent of both theDioscoreaspecies occupying 41% and 22% ofD. FloribundaandD. compositaessential oils, respectively. In this paper, we report for the first timeDioscoreaas a possible novel bioresource for the essential oil besides its well-known importance for yielding diosgenin.