pastoris strain gs115
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Genomic data mining reveals the transaminase repertoire of Komagataella phaffii (Pichia pastoris) strain GS115 and supports a systematic nomenclature

Journal of Genetics ◽

10.1007/s12041-020-01201-1 ◽

2020 ◽

Vol 99 (1) ◽

Author(s):

Maria-José Henríquez ◽

Rosalía Paula Cardós-Elena ◽

Darren Nicholas Nesbeth

Keyword(s):

Data Mining ◽

Pichia Pastoris ◽

Genomic Data ◽

Pastoris Strain ◽

Komagataella Phaffii ◽

Pastoris Strain Gs115 ◽

Systematic Nomenclature ◽

Genomic Data Mining

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Genotyping of recombinant Pichia pastoris strains

Cellular & Molecular Biology Letters ◽

10.2478/s11658-006-0029-z ◽

2006 ◽

Vol 11 (3) ◽

Cited By ~ 10

Author(s):

José Viader-Salvadó ◽

Eddy Cab-Barrera ◽

Luis Galán-Wong ◽

Martha Guerrero-Olazarán

Keyword(s):

Pichia Pastoris ◽

Recombinant Protein Production ◽

Protein Production ◽

Fragment Length Polymorphism ◽

Transformation Process ◽

Genetic Differences ◽

Host Strain ◽

Clonal Variation ◽

Recombinant Pichia Pastoris ◽

Pastoris Strain Gs115

AbstractA simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production.