Modern chromatographic method for estimating Loratadine and affections on healthcare

Purpose The purpose of this study is to establish Loratadine [LRD] quantification in purified and capsule formulations using a precise and specific Reversal Phase with a very high-performance liquid Chromatographic [RP-HPLC] technique. The approach was evaluated in agreement with the principles of the International Conference on Harmonization [ICH]. Arcus EP-C18 Ion Pac column, 5 m, 4.6 mm, 250 mm, mobile phase Methanol: Acetonitrile (60:40) v/v. Dibasic potassium phosphate buffer, pH 7.2, flow rate 1.0 ml/min. Design/methodology/approach The HPLC system used a 340 nm UV detector for testing. A 10-min run time was used for the analysis. At concentrations ranging from 2 to 10 g/ml, the technique was linear (R2 = 0.9998), exact (intra-day and inter-day relative standard deviation [RSD] values 1.0%), accurate (range recovery = 96%–102%), exclusive and strong. Findings The detecting and quantitation limits were 0.92 g/ml and 2.15 g/ml, respectively. Originality/value The findings demonstrated that the proposed method could accurately determine LRD in bulk and pill dose formats quickly and accurately.

Simultaneous Determination of Diloxanide Furoate and Metronidazole in Presence of Diloxanide Furoate Degradation Products

Three methods are presented for the simultaneous determination of diloxanide furoate (DLX) and metronidazole (MTR), used for their antiprotozoal and antiamoebic effect, in the presence of DLX alkaline degradates and in pharmaceutical formulations, without previous separation. The first method is chemometric-assisted spectrophotometry, in which principal component regression and partial least squares were applied. These two approaches were successfully applied to quantify each drug in the mixture using the information included in the absorption spectra in the range of 225–320 nm. The second method is TLC-densitometry, in which the binary mixture and degradates were separated on silica gel plates using a chloroform–acetone–glacial acetic acid (9.5 + 0.5 + 0.07, v/v/v) mobile phase and the bands were scanned at 254 nm. The last method is HPLC, in which DLX, MTR, and degradates were separated using the mobile phase acetonitrile–0.05 M dibasic potassium phosphate (25 + 75, v/v), adjusted to pH 4 with orthophosphoric acid, at a flow rate of 1 mL/min, on a C18 analytical column. Detection was at 254 nm. The proposed methods were successfully applied for the analysis of DLX and MTR in pharmaceutical formulations, and the results were statistically compared with a reported spectrophotometric method.

Spectrophotometric Determination of Roxarsone in Animal Feeds

Roxarsone is administered in the feed as a growth stimulant for chickens, turkeys, and swine. The drug is extracted with 2% dibasic potassium phosphate solution, the extract is centrifuged, the centrifugate is adjusted to the isoelectric point of proteins with hydrochloric acid, and the precipitated proteins are removed by centrifugation. Interferences are removed by treating the solution with activated charcoal at pH 12, which leaves a solution of roxarsone of intense color that can be measured spectrophotometrically at 410 nm. This method was subjected to a collaborative study. The average recovery from 13 collaborators was 99.25% with an average coefficient of variation of 6.91%. It is concluded that satisfactory results were reported and is therefore recommended that this method be adopted as official first action.

THE DECIDUAL CELL RESPONSES IN RABBITS

ABSTRACT Decidual responses were produced in the uteri of oestrogen-primed, progesterone treated, rabbits following traumatization of the uterus with a thread. Oestrone, administered along with progesterone, did not appear to augment the responses obtained with progesterone except, perhaps, at one dose level. Oestrone, injected into ligated segments of the uterus, was unable to stimulate decidual responses. Decidual responses were also obtained by injection of solutions of sodium carbonate and dibasic potassium phosphate into ligated segments of the uterus. Histamine dihydrochloride, HCI, heparin, serotonin and sodium carbonate were ineffective.