Assessment of single nucleotide polymorphism RS2596542 of MICA gene among nasopharyngeal cancer patients

MICA is an antigen that is commonly expressed on cell surface of tumoRS. MICA had a low expression level in normal cells but increased in pathological/cancer cells. The goal of the study was to determine the association of SNP RS2596542 with the risk of nasopharyngeal cancer (NPC). 144 biopsy samples diagnosed with NPC and 135 blood samples from healthy people were collected. Genotypes and alleles of RS2596542 MICA were determined by Realtime PCR genotyping. The genotype rate of MICA at RS2596542 in patients with NPC was 34.72% CC; 44.44% CT and 20.84% TT. The genotype of TT and T allele in the disease group was significantly higher than the control group (p=0.01*; OR=2.47; 95%CI: 1.22-4.97) and (p=0.01*; OR=1.51; 95%CI: 1.07-2.13). The T-allele at SNP RS2596542 of the MICA gene increases the risk of NPC and suggests that this could be used as a biomarker for NPC screening in Viet Nam. Keywords: MICA, RS2596542, nasopharyngeal cancer, NPC, Single Nucleotide Polymorphism, SNPs, Realtime PCR

Recurrent miscarriage in North Indian population: a study of association of polymorphisms in genes coding for the natural killer: cell receptor natural killer group 2, member D and its ligand MHC class I chain-related protein A

Background: The objective of this present study was to investigate the possible association of natural killer group (NKG) receptors gene polymorphisms and MHC class I chain-related protein A (MICA) gene polymorphism with recurrent spontaneous abortion (RSA).Methods: Three single-nucleotide polymorphism (SNPs) in NKG2D gene (rs2255336, rs2617160 and rs2617170) and one SNP in MICA gene (MICA129) rs1051792 were assessed in 100 controls and 100 patients employing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and agarose gel electrophoresis.Results: NKG2D (rs2617160) and MICA 129 (rs1051792) variants are associated with RSA risk in North Indian women.Conclusions: The NKG2D and MICA129 gene polymorphisms may influence the success of pregnancy in North Indian women population.