A model for the effects of germanium on silica biomineralization in choanoflagellates

Silica biomineralization is a widespread phenomenon of major biotechnological interest. Modifying biosilica with substances like germanium (Ge) can confer useful new properties, although exposure to high levels of Ge disrupts normal biosilicification. No clear mechanism explains why this disruption occurs. Here, we study the effect of Ge on loricate choanoflagellates, a group of protists that construct a species-specific extracellular lorica from multiple siliceous costal strips. High Ge exposures were toxic, whereas lower Ge exposures produced cells with incomplete or absent loricae. These effects can be ameliorated by restoring the germanium : silicon ratio, as observed in other biosilicifying organisms. We developed simulations of how Ge interacts with polymerizing silica. In our models, Ge is readily incorporated at the ends of silica forming from silicic acid condensation, but this prevents further silica polymerization. Our ‘Ge-capping’ model is supported by observations from loricate choanoflagellates. Ge exposure terminates costal strip synthesis and lorica formation, resulting in disruption to cytokinesis and fatal build-up of silicic acid. Applying the Ge-capping model to other siliceous organisms explains the general toxicity of Ge and identifies potential protective responses in metalloid uptake and sensing. This can improve the design of new silica biomaterials, and further our understanding of silicon metabolism.

The Separation of 32Silicon from Contaminating 3H and 60Co by Incorporation into Diatoms

32Si (β-. 0,1 MeV, half life about 280 years) has been used, as for as we are aware, for the first time in bio­ logical and biochemical experiments . 32Si was incorporated by the pathway of the silicon metabolism into shells of two diatom species (Cyclotella cryptica and Nitzschia spec.) and reisolated by dissolving the shells. Contaminating isotopes 3H and 60Co with 10000 times more activity were largely removed by this procedure.