spinner culture
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Cartilage ◽  
2018 ◽  
Vol 11 (1) ◽  
pp. 108-116 ◽  
Author(s):  
Mark W. Grzanna ◽  
Erica J. Secor ◽  
Lowella V. Fortuno ◽  
Angela Y. Au ◽  
Carmelita G. Frondoza

Objective Osteoarthritis is a painful, chronic joint disease affecting man and animals with no known curative therapies. Palliative nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used but they cause adverse side effects prompting the search for safer alternatives. To address this need, we evaluated the anti-inflammatory activity of avocado/soybean unsaponifiables (ASU), glucosamine (GLU), and chondroitin sulfate (CS) with or without the NSAID carprofen. Design Canine chondrocytes were propagated in microcarrier spinner culture and incubated with (1) control medium, (2) ASU (8.3 µg/mL) + GLU (11 µg/mL) + CS (20 µg/mL) combination for 24 hours; and/or carprofen (40 ng/mL). Cultures were next incubated with control medium alone or IL-1β (10 ng/mL) for another 24 hours. Production of PGE2, IL-6, IL-8, and MCP-1 (also known as CCL-2) were measured by ELISA. Results Chondrocytes proliferated in microcarrier spinner culture and produced type II collagen and aggrecan. Stimulation with IL-1β induced significant increases in PGE2, IL-6, IL-8, and MCP-1 production. The increases in production were suppressed by carprofen as well as [ASU+GLU+CS]. The combination of carprofen and [ASU+GLU+CS] reduced PGE2 production significantly more than either preparation alone. The inhibitory effect of carprofen on IL-6, IL-8, and MCP-1 production was significantly less than that of [ASU+GLU+CS], whereas the combination did not reduce the production of these molecules significantly more than [ASU+GLU+CS] alone. Conclusions The potentiating effect of [ASU+GLU+CS] on low-dose carprofen was identified in chondrocyte microcarrier spinner cultures. Our results suggest that the combination of low-dose NSAIDs like carprofen with [ASU+GLU+CS] could offer a safe, effective management for joint pain.


2014 ◽  
Vol 5 (5) ◽  
pp. 110 ◽  
Author(s):  
Alan Tin-Lun Lam ◽  
Allen Kuan-Liang Chen ◽  
Jian Li ◽  
William R Birch ◽  
Shaul Reuveny ◽  
...  

2011 ◽  
Vol 345 (3) ◽  
pp. 415-423 ◽  
Author(s):  
Ji Suk Choi ◽  
Beob Soo Kim ◽  
Jae Dong Kim ◽  
Young Chan Choi ◽  
Eun Kyu Lee ◽  
...  

2008 ◽  
Vol 17 (10-11) ◽  
pp. 1231-1242 ◽  
Author(s):  
Ortwin Naujok ◽  
Flavio Francini ◽  
Sally Picton ◽  
Anne Jörns ◽  
Clifford J. Bailey ◽  
...  

Mouse embryonic stem (ES) cells have the potential to differentiate into insulin-producing cells, but efficient protocols for in vitro differentiation have not been established. Here we have developed a new optimized four-stage differentiation protocol and compared this with an established reference protocol. The new protocol minimized differentiation towards neuronal progeny, resulting in a population of insulin-producing cells with β-cell characteristics but lacking neuronal features. The yield of glucagon and somatostatin cells was negligible. Crucial for this improved yield was the removal of a nestin selection step as well as removal of culture supplements that promote differentiation towards the neuronal lineage. Supplementation of the differentiation medium with insulin and fetal calf serum was beneficial for differentiation towards monohormonal insulin-positive cells. After implantation into diabetic mice these insulin-producing cells produced a time-dependent improvement of the diabetic metabolic state, in contrast to cells differentiated according to the reference protocol. Using a spinner culture instead of an adherent culture of ES cells prevented the differentiation towards insulin-producing cells. Thus, prevention of cell attachment in a spinner culture represents a means to keep ES cells in an undifferentiated state and to inhibit differentiation. In conclusion, this study describes a new optimized four-stage protocol for differentiating ES cells to insulin-producing cells with minimal neuronal cell formation.


2006 ◽  
Vol 14 ◽  
pp. S111 ◽  
Author(s):  
C.G. Frondoza ◽  
J.L. Demko ◽  
P.V. Phan ◽  
E.A. Kramer ◽  
R. McLaughlin

2004 ◽  
Vol 18 (2) ◽  
pp. 105-112 ◽  
Author(s):  
Alan H. Shikani ◽  
David J. Fink ◽  
Afshin Sohrabi ◽  
Phong Phan ◽  
Anna Polotsky ◽  
...  
Keyword(s):  

Author(s):  
Michael Overstreet ◽  
Afshin Sohrabi ◽  
Anna Polotsky ◽  
David Hungerford ◽  
Carmelita Frondoza

Author(s):  
MICHAEL OVERSTREET ◽  
AFSHIN SOHRABI ◽  
ANNA POLOTSKY ◽  
DAVID S. HUNGERFORD ◽  
CARMELITA G. FRONDOZA

Author(s):  
Beno�t Y. Bouchet ◽  
Mirylsa Col�n ◽  
Anna Polotsky ◽  
Alan H. Shikani ◽  
David S. Hungerford ◽  
...  

1994 ◽  
Vol 267 (2) ◽  
pp. C473-C481 ◽  
Author(s):  
A. Z. Wang ◽  
J. C. Wang ◽  
G. K. Ojakian ◽  
W. J. Nelson

Madin-Darby canine kidney epithelial cells form three-dimensional cysts in spinner culture with a defined cell surface polarity. Transfer of cysts from spinner culture to a collagen gel matrix results in rapid loss of apical membrane proteins from the outside surface of the cyst, degradation of extracellular matrix (ECM) from the cyst lumen, and de novo formation of the apical membrane at the luminal surface. Degradation of endogenous ECM was inhibited with 1,10-phenanthroline, an inhibitor of metalloproteinases, resulting in cysts in which cells are surrounded by either cell-cell or cell-substratum contacts. The consequence of the lack of a free cell surface on the formation of a new apical membrane domain in these cysts was analyzed. Changes in cell surface polarity were followed with antibodies to marker proteins of the apical or basolateral membranes. In the absence of a free cell surface, the apical membrane formed de novo by accumulation and fusion of presorted vesicles containing apical membrane proteins; the coalescence of these vesicles results in the formation of a central lumen. These results provide novel insights into the generation of membrane domains and formation of a lumen in complex, three-dimensional epithelial structures in development.


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