The effect of the drug “Amiksyn” on the immune status of carp

The productivity of pond fish, in particular carp, largely depends on the intensity of use of aquatic bioresources. The aim of the study was to investigate the effect of “Amiksyn” on the immunological parameters of carp fish, namely: the number of T- and B-lymphocytes, immunoglobulins, the level of circulating immune complexes, lysozyme, phagocytic activity and bactericidal activity of serum. 10 fish were selected for hematological and biochemical studies. The research was conducted in the aquarium of the Department of Aquatic Bioresources of Stepan Gzhytskyi NUVMB Lviv. Fish were kept in the pool for 21 days to adapt to the new conditions, and at the beginning of the series of experiments were placed in aquariums with a volume of 200 liters. Aeration and mechanical filtration of water were provided in the tanks. The temperature during the experiments fluctuated slightly and was 18 ± 1.5 °C. The main hydrochemical parameters corresponded to fish farming standards. In order to adjust the immune response, and thus prevent possible manifestations of fish damage by pathogens, we studied the possibility of using the immunostimulant “Amiksyn”, which was used for 5 – 10 – 15 – 20 days and then dosing 5 – 10 – 15 mg/kg body weight Pisces. The use of “Amiksyn” at a dose of 10 mg/kg of fish weight four times during the fifteen-day period with an interval of 5 days provided a decrease in the number of T-lymphocytes by 9.1 % and B-lymphocytes by 23.0 %; caused the activation of non-specific resistance indicators, namely lysozyme activity increased by 28.7 %, phagocytic activity of leukocytes increased by 14.0 % and bactericidal activity of serum increased by 19.0 %. Therefore, to adjust the immune system and stimulate the metabolic processes of fish in order to prevent the negative effects of various pathogens, the most optimal dose was 10 mg/kg of fish weight within 15 days of use.

Jambal roti characteristics: a traditional fermented fish from Rembang, Central Java

Jambal roti is a fermented fish product, commonly found in some regions in Java Island, Indonesia, such as Pekalongan, Cilacap, Pangandaran, Rembang, and along the northern coast. The raw sea catfish (Arius thalassinus) were traditionally processed by salting (salt amounting to 30% of the fish weight) then sun-drying. This study examined the microbiological and chemical characteristics of jambal roti produced in the Rembang Regency. Samples were taken from artisanal jambal roti producers during the making process. The proximate and chemical (final product of jambal roti) test results showed that the aw (0.723) and salt contents (18.166%) of the samples met the SNI 2721:2009, but the water (48.534%), ash (18.022%), and dissolved protein contents (5.494%) were above their allowable levels. The microbial analysis revealed that despite the absence of Escherichia coli and Salmonella spp. in the product, the total aerobic bacteria (APC) (6.6×102 CFU/g) and yeast and mold contamination (1.4×103 CFU/g) found in the samples were above the standards.

TEKNIS PRODUKSI PEMBENIHAN DAN PENDEDERAN IKAN LEMON ALGAE EATER Gyrinocheilus aymoneiri DI ADE’S FISH FARM, KABUPATEN BOGOR, JAWA BARAT

ABSTRACTLemon fish Algae Eater is one of the aquarium fish which is well-known as Algae Eater. The Lemon Algae Eater is placed in a pond measuring 2 m x 3 m x 0.8 m, with a water height of 50 cm. For optimum health, feed the Algae Eater with Feng Li pellets by having a restricted feeding method or this can also be limited to 5% from the brood fish weight. The semi-natural hatchery of Lemon fish Algae Eater is needed. An aquarium (100cm x 60 cm x 50 cm) with a water height of 30 cm is needed for the hatchery of Lemon fish Algae Eater eggs. The average fecundity of Lemon fish Algae Eater eggs produced in 1 spawning can reache up to 84 742 eggs with the average of Fertilization Rate is 79%, the Hatching Rate is 83%, and the Survival Rate is 74%. The Lemon Algae Eater eggs will hatch in 10-12 hours. Fish nursery of lemon fish algae eater is used pond measures 3 m x 2 m x 1.5 m. The freshly hatched larvae are harvested at the age of 30 days and 45 days with a size of 1.2-1.5 cm/fish and 2.5-3 cm/fish. ABSTRAKLemon algae eater merupakan salah satu ikan aquarium yang dikenal sebagai pemakan alga. Pemeliharaan ikan lemon algae eater dilakukan pada bak beton yang berukuran 2 m x 3 m x 0.8 m, dengan tinggi air 50 cm. Pakan yang diberikan berupa pelet feng li dengan metode pemberian pakan restricted atau metode dibatasi dengan 5% dari bobot induk. Pemijahaan induk ikan lemon algae eater dilakukan secara semi alami. Wadah penetasan telur ikan lemon algae eater menggunakan akuarium ukuran 100 cm x 60 cm x 50 cm dengan tinggi air 30 cm. Rata-rata fekunditas telur ikan lemon algae eater yang dihasilkan dalam 1 kali pemijahan mencapai 84 742 butir telur dengan memiliki nilai rata-rata Fertilization Rate sebesar 79%, Hatching Rate sebesar 83%, dan Survival Rate sebesar 74%. Telor ikan lemon algae eater akan menetas dalam waktu 10-12 jam. Kegiatan pendederan ikan lemon algae eater menggunakan wadah bak beton yang berukuran 3 m x 2 m x 1.5 m. Benih yang dipanen berumur 30 hari dan 45 hari dengan ukuran 1.2 -1.5 cm/ekor dan 2.5 - 3 cm/ekor.

Weight-dependent susceptibility of tilapia to tilapia lake virus infection

The emergence of tilapia lake virus (TiLV) has had a severely negative impact on global tilapia aquaculture. TiLV infection has been reported at different life stages of tilapia, with more emphasis on fry and fingerlings; however, the virulence and pathology of TiLV at different tilapia size remains unexplored. In this study, tilapias from a single spawning were raised to 5 g, 25 g, and 65 g, and subsequently challenged by the intraperitoneal injection and cohabitation of a virulent strain of TiLV. The cumulative mortality, viral load, and histopathology of the fish were determined until 22 days post-infection (dpi). The cumulative mortality of the 5 g, 25 g, and 65 g fish was 85% (±1.67), 55% (±2.89), and 51.67% (±7.49), respectively. At 14 dpi, the mean TiLV load in the liver of the 5 g fish was significantly higher than in the 25 g and 65 g fish. All the weight groups showed severe pathological changes in the liver, spleen, and intestine after TiLV infection, but no particular difference was otherwise noted during the study with the exception of higher pathological scores in the liver of the small fish at 14 dpi. Overall, this study indicated that small fish are more susceptible to TiLV infection than large fish. Although multiple factors, including environmental factors, farm management practices, strains of virus could contribute to different susceptibility of fish to viral infection, the present study provides the evidence to support that fish weight affects the mortality and clinical outcome during TiLV infection. More intensive measures such as strict biosecurity and disease surveillance during the susceptible weight should therefore be emphasized to reduce the impact of this virus.

KERAGAAN BIOREPRODUKSI TIGA GENERASI IKAN TAMBAKAN (Helostoma temminckii Cuvier, 1829)

Ikan tambakan merupakan ikan lokal yang potensial menjadi ikan budidaya. Salah satu proses penting dalam budidaya adalah aspek reproduksi. Penelitian untuk mengetahui karakteristik bioreproduksi tiga generasi ikan tambakan hasil kegiatan domes‘tikasi di Balai Riset Perikanan Budidaya Air Tawar dan Penyuluhan Perikanan, Bogor. Pengujian dilakukan untuk mengetahui posisi gonad, fekunditas, derajat pembuahan, dan derajat penetasan ikan tambakan generasi yang berbeda. Hasil yang diperoleh menunjukkan posisi gonad vertikal mengarah ke tulang belakang, di bagian ujung melengkung ke arah depan dan gonad berada di belakang urogenital. Fekunditas telur/gram bobot ikan yang dihasilkan tidak berbeda antar generasi dengan jumlah berkisar antara 76 ± 13 sampai 83 ± 14 butir. Persentase telur terbuahi dan telur menetas antar generasi juga tidak berbeda, masing-masing berkisar antara antara 70,6 ± 16,05 sampai 92,9 ± 10,88%; dan 51,3 ± 16,64 sampai 74,3 ± 10,32%. Jumlah larva yang dihasilkan juga tidak berbeda nyata antar generasi, berurutan dari yang kecil ke besar G-0 (38,7 ± 10,72 butir/g bobot ikan), G-1 (45,3 ± 5,20 butir/g bobot ikan), dan G-2 (55,7 ± 7,75 butir/g bobot ikan). Program domestikasi ikan tambakan tidak mempengaruhi karakter reproduksi ikan antar generasi. Keragaman karakter reproduksi antar generasi masih tinggi. Pembentukan generasi selanjutnya perlu dipertahankan, sehingga dapat digunakan sebagai ikan budidaya baru.Kissing gourami is an Indonesian local fish species that has the potential to be developed as a farmed fish. However, the current domestication stage of the fish still requires further information regarding its bio reproduction. The research on determining the bioreproductive characteristics of three generations of kissing gourami has been carried out at the Research Institute of Freshwater Aquaculture and Fisheries Extensions, Bogor. This research aimed to determine the gonad position, gonad maturity index, and fecundity between the kissing gourami generations. The results showed that the gonads were positioned vertically towards the backbone, curved ends towards the front and located behind the urogenital. The fecundity of produced eggs/gram body weight of fish did not differ between generations and ranged between 76 ± 13–83 ± 14 eggs. The percentages of fertilized eggs and hatched eggs between generations did not differ and ranged between 70.6 ± 16.05–92.9 ± 10.88% and 51.3 ± 16.64–74.3 ± 10.32%, respectively. The number of produced larvae was also not significantly different between generations where G-0 has the lowest number (38.7 ± 10.72 eggs/g fish weight), followed by G-1 (45.3 ± 5.20 eggs/g fish weight) and G-2 has the highest number of produced larvae (55.7 ± 7.75 eggs/g fish weight). The aquaculture domestication program does not affect the reproductive character of fish between generations. The diversity of reproductive characters between generations is still high. The formation of the next generation needs to be maintained, so that it can be used as a new cultured fish.

ASPEK BIOLOGI IKAN SIRO (Amblygaster sirm) BERDASARKAN DATA DI PELABUHAN PERIKANAN PANTAI TASIKAGUNG, REMBANG

Spotted sardinella is one of small pelagic fish with high economic protein and it has been caught by mini purse seine. In fact, Spotted sardinella has a large number of catches which can endanger their sustainability in Java Sea. The purpose of the study was to find out the length of first caught, length and weight relationship, growth parameters, mortality rate, and exploitation rate of spotted sardinella. The method used was survey method. The data were collected once in every 1 month during August until November 2021 at Tasikagung Fishery Port. The primary data collected were fish total length (mmTL) and fish weight (g). The result of the study revealed that size structure of fish ranged from 120-205 mmTL and length first capture 170 mmTl. The length-weight relationship analysis was W = 0,00000571 L 3,54 with positive allometric growth pattern. The growth parameters by Von Bertanlanffy was Lt = 206,5(1-e-0,95(t+0,00266)) and it was categorized as fast growth. The fishing mortality and natural mortality were 1,71 year-1 and 1,01 year-1. The exploitation rate was 0,63 year-1, which means sardinella has been exploited.

The effect of water level in vertical aquaculture systems on production performance, biochemistry, hematology, and histology of Anguilla bicolor bicolor

The water level in the cultivation of eel (Anguilla bicolor bicolor) is an important study in order to provide the optimal water level for cultivation. Optimizing the water level will affect the substitution of respiration energy with energy to grow. In addition, the water level information is related to the efficiency of water use for eel production in the future. Information on water level for eel production is still very limited, so this research is necessary to do. A total of 120 eel elver (initial weight 13.66 ± 0.09 g) were collected from eel companies in Bogor City, Indonesia. Fish were reared in vertical aquaculture systems with a stocking density of 10 fish per container for 60 days. The artificial feed containing 55% protein given as much as 3–5% of the biomass. Absorption and water replacement were done 20% per day. The result of this research showed that fish weight increased with an average of 33.45 ± 0.33 g. Different water levels had an impact to KKb, SGOT, ALP, and He. There was erosion of the skin epidermis and necrosis of the gill filaments due to the adaptation process. Water quality was within the optimum range for all treatments and 1.5 cm water level is recommended for maintenance (SGOT, ALP and He values were closest to normal values).

Variation in the Proximate Composition, Amino Acids Content and Fatty Acids of Thai Pangus (Pangasianodonhypophthalmus) Fish Depending on Size

The nutritional characteristics such as proximate composition, amino acids, and fatty acids properties of fish depend on the size of fish. Determination of the nutritional parameters of Thai pangus muscle depending on five different sizes, viz., 0.5 kg, 1.0 kg, 1.5 kg, 2.0 kg, and 2.5 kg showed that the moisture content was decreased whereas the protein content was increased with the increment of fish weight. The protein content was the highest 16.60% in 2.5 kg size fish and the lowest content 14.75% was in 0.5 kg size fish. The lipid content was found to increase significantly (P≤0.05) with the increment of fish weight. There were eight essential and nine non-essential amino acids available and the contents were found to increase with the increment of fish weight. The content of essential amino acids was the highest (14.43 g/100 g fish muscle) in 2.5 kg size fish while the lowest (7.79 g/100 g fish muscle) in 0.5 kg size fish. Total 13 fatty acids were detected in fish oil among which oleic acid content was the highest (44.55% to 47.10%) followed by palmitic acid (25.78% to 36.39%), however, the ω-3 polyunsaturated fatty acids contentwere very poor. The saturated fatty acids content were found to increase whereas the polyunsaturated fatty acids content were found to decrease with increasing the size of fish. So, the findings of this present study reveal that the muscles of Thai Pangus fish are considered as highly nutritive and healthy.