A LIMNOLOGICAL STUDY OF PRIMARY PRODUCTIVITY IN SEASONAL VARIATIONS OF A TYPICAL TROPICAL POND OF BUNDELKHAND REGION, INDIA

The primary productivity of the Mahil pond has been estimated from March 2017 to February 2018 at four different stations. Various physicochemical parameters and phytoplankton were studied. The seasonal variation of primary productivity revealed that maximum and minimum values of Gross primary productivity and community respiration were associated with rainy and summer seasons respectively. The minimum values of Net primary productivity were recorded during rainy season and maximum during winterfor different study stations.

Detection of the oil-producing microalga Botryococcus braunii in natural freshwater environments by targeting the hydrocarbon biosynthesis gene SSL-3

The green microalga Botryococcus braunii produces hydrocarbon oils at 25–75% of its dry weight and is a promising source of biofuel feedstock. Few studies have examined this species’ ecology in natural habitats, and few wild genetic resources have been collected due to difficulties caused by its low abundance in nature. This study aimed to develop a real-time PCR assay for specific detection and quantification of this alga in natural environments and to quantify spatiotemporal variations of wild B. braunii populations in a tropical pond. We designed PCR primers toward the hydrocarbon biosynthesis gene SSL-3 and examined amplification specificity and PCR efficiency with 70 wild strains newly isolated from various environments. The results demonstrated that this PCR assay specifically amplified B. braunii DNA, especially that of B-race strains, and can be widely used to detect wild B. braunii strains in temperate and tropical habitats. Field-testing in a tropical pond suggested a diurnal change in the abundance of B. braunii in surface water and found B. braunii not only in surface water, but also at 1–1.5 m deep and in bottom sediments. This method can contribute to efficient genetic resource exploitations and may also help elucidate the unknown ecology of B. braunii.